Article
Multidisciplinary Sciences
Xiaoqian Zhang, Chang Li, Bingzhou Zhang, Zhonghua Li, Wei Zeng, Rui Luo, Jiyue Cao, Guofu Cheng, Shengxian Fan, Qigai He
Summary: The study compared the differential expression of miRNA and mRNA in swine testicular cells infected with variant virulent and attenuated vaccine-like strains of PEDV. Significant differences were found in host immune responses and viral pathogenicity regulation, providing potential novel strategies for the prevention and control of PED.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Dorian Forte, Martina Barone, Cristina Morsiani, Giorgia Simonetti, Francesco Fabbri, Samantha Bruno, Erika Bandini, Daria Sollazzo, Salvatore Collura, Maria Chiara Deregibus, Giuseppe Auteri, Emanuela Ottaviani, Nicola Vianelli, Giovanni Camussi, Claudio Franceschi, Miriam Capri, Francesca Palandri, Michele Cavo, Lucia Catani
Summary: In this study, it was found that the absolute numbers of circulating CD34(+) cells were significantly increased in triple-negative (TN) myelofibrosis patients. These cells displayed in vitro defective functions and were unresponsive to the inflammatory microenvironment. Furthermore, the circulating extracellular vesicles (EVs) from TN patients were found to promote the survival of TN CD34(+) cells, suggesting a distinct EV-driven signal promoting malignant hemopoiesis in TN patients. The distinct gene expression profiling analysis and microRNA signature in TN patients may provide insights for novel therapeutic approaches for this rare form of myelofibrosis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Immunology
Atila Duque Rossi, Luiza Mendonca Higa, Alice Laschuk Herlinger, Marcelo Ribeiro-Alves, Mariane Talon de Menezes, Ana Lucia Moraes Giannini, Cynthia Chester Cardoso, Andrea T. T. Da Poian, Amilcar Tanuri, Renato Santana Aguiar
Summary: An analysis of the expression levels of 754 human microRNAs in DENV-infected THP-1 cells revealed differential expression of 11 human microRNAs, with gene ontology and enrichment analysis highlighting potential biological processes affected by these molecules.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anne Aries, Celine Zanetti, Philippe Henon, Bernard Drenou, Rachid Lahlil
Summary: Injecting ex vivo monitored human CD34(+) stem cells into myocardial scar tissue has shown real benefits for patients recovering from myocardial infarctions. However, debates on the potential efficacy of these stem cells in cardiac regenerative therapies remain. This study aimed to elucidate the levels of CD34(+) stem cell implication in cardiac regeneration and identified main regulators and genes involved in their differentiation and secretion processes. The findings showed that stimulated CD34(+) stem cells have the capacity to express cardiac markers and could potentially be primed towards cardiovascular cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Leonie F. H. Fransen, Martin O. Leonard
Summary: Myeloid cells are crucial in initial responses to environmental hazards, but cells derived from induced pluripotent stem cells (iPSC) may lack a fully mature phenotype to adequately respond to environmental exposures.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Jianmin Li, Chanyuan Pan, Chao Tang, Wenwen Tan, Weiwei Zhang, Jing Guan
Summary: The miR-184/TP63 axis was found to modulate TGF-beta 1-induced fibrotic alterations in epithelial cell lines and bleomycin-induced pulmonary fibrosis in mice, confirming its involvement in the progression of IPF.
LABORATORY INVESTIGATION
(2021)
Editorial Material
Biochemistry & Molecular Biology
Amy H. Buck
Summary: In their recent Nature paper, Garcia-Martin et al. demonstrate that the sequences within a microRNA play a role in determining the amount of microRNA transferred to another cell via extracellular vesicles. This finding supports the growing evidence of cellular communication through RNA, but the mechanisms underlying how cells listen remain poorly understood.
Review
Medicine, Research & Experimental
Jinyu Li, Xiaohui Xu, Jiane Liu, Sudan Zhang, Xiaohua Tan, Zhiqiang Li, Jian Zhang, Zheng Wang
Summary: Autism spectrum disorder (ASD) is a congenital mental disorder that is influenced by genetic and environmental factors. Recent research shows that microRNAs (miRNAs) play a significant role in the onset and progression of ASD, and have the potential to serve as biomarkers and therapeutic targets for ASD diagnosis and treatment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Biochemistry & Molecular Biology
Antonio Pellegrino, Sophie-Charlotte Fabig, Dilara Kersebaum, Philipp Huellemann, Ralf Baron, Toralf Roch, Nina Babel, Harald Seitz
Summary: This study identified four microRNAs that may be linked to degenerative processes, blood flow regulation, and signal transduction, potentially leading to chronic painful PNP. These microRNAs may serve as potential novel biomarkers and have therapeutic implications.
Article
Biochemistry & Molecular Biology
Jiamin Wang, Lixia Liu, Di Yang, Li Zhang, Ayimuguli Abudureyimu, Zilin Qiao, Zhongren Ma
Summary: This study revealed the role of miRNA in the tumorigenic phenotype of MDCK cell line. The miRNA expression profiles of MDCK cells with different tumorigenicity were characterized and investigated. The results showed that certain miRNAs may influence the tumorigenicity of MDCK cells by regulating target genes. These findings provide important insights into the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.
Article
Cell Biology
Ziyue Mi, Li Gong, Yujie Kong, Peizhe Zhao, Yonghua Yin, Haixia Xu, Li Tian, Zhong Liu
Summary: This study examines the expression profiles of exosomal microRNAs in apheresis platelet concentrates during storage and predicts the potential functions of target genes. The results show that differentially expressed microRNAs are involved in positive regulation of cell proliferation and nervous system development. They are mainly enriched in signaling pathways related to the regulation of pluripotency of stem cells, prolactin, and FoxO. Understanding the role of exosomal microRNAs in platelet transfusion can help improve the safety and efficacy of this clinical treatment.
Article
Neurosciences
Ali Jason Saleh, Hans Gerd Nothwang
Summary: A comparative study of miRNA expression during development of the auditory hindbrain in mammals and chickens revealed both shared and distinct features. In the nucleus magnocellularis, differential expression patterns along the cochleotopic axis were observed, with miRNAs reflecting different rhombomeric origins. Bioinformatics analysis predicted mRNA targets associated with neuronal developmental processes. Overall, this study adds miRNA patterns to the list of cochleotopic and developmental gradients in the central auditory system.
JOURNAL OF COMPARATIVE NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sarah Janati-Idrissi, Mariana Roza de Abreu, Cervin Guyomar, Fernanda de Mello, Thaovi Nguyen, Nazim Mechkouri, Stephanie Gay, Jerome Montfort, Anne Alicia Gonzalez, Marzieh Abbasi, Jerome Bugeon, Violette Thermes, Herve Seitz, Julien Bobe
Summary: This study provides a proof of concept for the successful identification of miRNA phenotypic targets from RNA-seq data. The researchers identified tead3b as a target of miR-202-5p, which is involved in ovarian function and egg production. This study highlights the importance of understanding miRNA functions and their targets in order to better understand biological processes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Toxicology
Leonie F. H. Fransen, Martin O. Leonard
Summary: The study explored the differential responses of immune cells to Paraquat (PQ), a redox cycling herbicide known for acute toxicity in humans. RNA-Seq profiling was used to establish primary cultures of human CD34+ stem cell derived macrophages (MCcd34) and dendritic cells (DCcd34), revealing increased cytotoxicity within DCcd34 cultures in response to PQ. The study also found that PQ induced unfolded protein response (UPR), p53, Irf and DC maturation genes in DCcd34 but not in MCcd34. Further examination showed enhanced inflammatory responses within MCcd34 but not DCcd34 when co-treated with PQ and the common allergen house dust mite (HD)。
TOXICOLOGY IN VITRO
(2021)
Article
Chemistry, Analytical
Hua Chai, Yuguo Tang, Peng Miao
Summary: Ultra-sensitive electrochemical analysis of miRNA is achieved through the construction of a three-dimensional DNA tetrahedral nanostructure, and successfully applied for the detection of miRNA in cell and serum samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Karla Svobodova, Halka Lhotska, Lucie Hodanova, Lenka Pavlistova, Denisa Vesela, Monika Belickova, Jitka Vesela, Jana Brezinova, Iveta Sarova, Silvia Izakova, Libuse Lizcova, Magda Siskova, Anna Jonasova, Jaroslav Cermak, Kyra Michalova, Zuzana Zemanova
GENES CHROMOSOMES & CANCER
(2020)
Article
Cell Biology
Andrea Hrustincova, Zdenek Krejcik, David Kundrat, Katarina Szikszai, Monika Belickova, Pavla Pecherkova, Jiri Klema, Jitka Vesela, Monika Hruba, Jaroslav Cermak, Tereza Hrdinova, Matyas Krijt, Jan Valka, Anna Jonasova, Michaela Dostalova Merkerova
Article
Biochemistry & Molecular Biology
Elsa Bernard, Yasuhito Nannya, Robert P. Hasserjian, Sean M. Devlin, Heinz Tuechler, Juan S. Medina-Martinez, Tetsuichi Yoshizato, Yusuke Shiozawa, Ryunosuke Saiki, Luca Malcovati, Max F. Levine, Juan E. Arango, Yangyu Zhou, Francesc Sole, Catherine A. Cargo, Detlef Haase, Maria Creignou, Ulrich Germing, Yanming Zhang, Gunes Gundem, Araxe Sarian, Arjan A. van de Loosdrecht, Martin Jadersten, Magnus Tobiasson, Olivier Kosmider, Matilde Y. Follo, Felicitas Thol, Ronald F. Pinheiro, Valeria Santini, Ioannis Kotsianidis, Jacqueline Boultwood, Fabio P. S. Santos, Julie Schanz, Senji Kasahara, Takayuki Ishikawa, Hisashi Tsurumi, Akifumi Takaori-Kondo, Toru Kiguchi, Chantana Polprasert, John M. Bennett, Virginia M. Klimek, Michael R. Savona, Monika Belickova, Christina Ganster, Laura Palomo, Guillermo Sanz, Lionel Ades, Matteo Giovanni Della Porta, Alexandra G. Smith, Yesenia Werner, Minal Patel, Agnes Viale, Katelynd Vanness, Donna S. Neuberg, Kristen E. Stevenson, Kamal Menghrajani, Kelly L. Bolton, Pierre Fenaux, Andrea Pellagatti, Uwe Platzbecker, Michael Heuser, Peter Valent, Shigeru Chiba, Yasushi Miyazaki, Carlo Finelli, Maria Teresa Voso, Lee-Yung Shih, Michaela Fontenay, Joop H. Jansen, Jose Cervera, Yoshiko Atsuta, Norbert Gattermann, Benjamin L. Ebert, Rafael Bejar, Peter L. Greenberg, Mario Cazzola, Eva Hellstrom-Lindberg, Seishi Ogawa, Elli Papaemmanuil
Article
Oncology
Katarina Szikszai, Zdenek Krejcik, Jiri Klema, Nikoleta Loudova, Andrea Hrustincova, Monika Belickova, Monika Hruba, Jitka Vesela, Viktor Stranecky, David Kundrat, Pavla Pecherkova, Jaroslav Cermak, Anna Jonasova, Michaela Dostalova Merkerova
Article
Chemistry, Medicinal
Hana Votavova, Zuzana Urbanova, David Kundrat, Michaela Dostalova Merkerova, Martin Vostry, Monika Hruba, Jaroslav Cermak, Monika Belickova
Summary: DFX improves hematopoiesis in anemic myelodysplastic syndrome patients by modulating immune response, suppressing negative regulators of blood cell differentiation, and upregulating heme metabolism.
Correction
Biochemistry & Molecular Biology
Elsa Bernard, Yasuhito Nannya, Robert P. Hasserjian, Sean M. Devlin, Heinz Tuechler, Juan S. Medina-Martinez, Tetsuichi Yoshizato, Yusuke Shiozawa, Ryunosuke Saiki, Luca Malcovati, Max F. Levine, Juan E. Arango, Yangyu Zhou, Francesc Sole, Catherine A. Cargo, Detlef Haase, Maria Creignou, Ulrich Germing, Yanming Zhang, Gunes Gundem, Araxe Sarian, Arjan A. van de Loosdrecht, Martin Jadersten, Magnus Tobiasson, Olivier Kosmider, Matilde Y. Follo, Felicitas Thol, Ronald F. Pinheiro, Valeria Santini, Ioannis Kotsianidis, Jacqueline Boultwood, Fabio P. S. Santos, Julie Schanz, Senji Kasahara, Takayuki Ishikawa, Hisashi Tsurumi, Akifumi Takaori-Kondo, Toru Kiguchi, Chantana Polprasert, John M. Bennett, Virginia M. Klimek, Michael R. Savona, Monika Belickova, Christina Ganster, Laura Palomo, Guillermo Sanz, Lionel Ades, Matteo Giovanni Della Porta, Alexandra G. Smith, Yesenia Werner, Minal Patel, Agnes Viale, Katelynd Vanness, Donna S. Neuberg, Kristen E. Stevenson, Kamal Menghrajani, Kelly L. Bolton, Pierre Fenaux, Andrea Pellagatti, Uwe Platzbecker, Michael Heuser, Peter Valent, Shigeru Chiba, Yasushi Miyazaki, Carlo Finelli, Maria Teresa Voso, Lee-Yung Shih, Michaela Fontenay, Joop H. Jansen, Jose Cervera, Yoshiko Atsuta, Norbert Gattermann, Benjamin L. Ebert, Rafael Bejar, Peter L. Greenberg, Mario Cazzola, Eva Hellstrom-Lindberg, Seishi Ogawa, Elli Papaemmanuil
Correction
Biochemistry & Molecular Biology
Elsa Bernard, Yasuhito Nannya, Robert P. Hasserjian, Sean M. Devlin, Heinz Tuechler, Juan S. Medina-Martinez, Tetsuichi Yoshizato, Yusuke Shiozawa, Ryunosuke Saiki, Luca Malcovati, Max F. Levine, Juan E. Arango, Yangyu Zhou, Francesc Sole, Catherine A. Cargo, Detlef Haase, Maria Creignou, Ulrich Germing, Yanming Zhang, Gunes Gundem, Araxe Sarian, Arjan A. van de Loosdrecht, Martin Jadersten, Magnus Tobiasson, Olivier Kosmider, Matilde Y. Follo, Felicitas Thol, Ronald F. Pinheiro, Valeria Santini, Ioannis Kotsianidis, Jacqueline Boultwood, Fabio P. S. Santos, Julie Schanz, Senji Kasahara, Takayuki Ishikawa, Hisashi Tsurumi, Akifumi Takaori-Kondo, Toru Kiguchi, Chantana Polprasert, John M. Bennett, Virginia M. Klimek, Michael R. Savona, Monika Belickova, Christina Ganster, Laura Palomo, Guillermo Sanz, Lionel Ades, Matteo Giovanni Della Porta, Alexandra G. Smith, Yesenia Werner, Minal Patel, Agnes Viale, Katelynd Vanness, Donna S. Neuberg, Kristen E. Stevenson, Kamal Menghrajani, Kelly L. Bolton, Pierre Fenaux, Andrea Pellagatti, Uwe Platzbecker, Michael Heuser, Peter Valent, Shigeru Chiba, Yasushi Miyazaki, Carlo Finelli, Maria Teresa Voso, Lee-Yung Shih, Michaela Fontenay, Joop H. Jansen, Jose Cervera, Yoshiko Atsuta, Norbert Gattermann, Benjamin L. Ebert, Rafael Bejar, Peter L. Greenberg, Mario Cazzola, Eva Hellstrom-Lindberg, Seishi Ogawa, Elli Papaemmanuil
Article
Oncology
Pavla Koralkova, Monika Belickova, David Kundrat, Michaela Dostalova Merkerova, Zdenek Krejcik, Katarina Szikszai, Monika Kaisrlikova, Jitka Vesela, Pavla Vyhlidalova, Jan Stetka, Alzbeta Hlavackova, Jiri Suttnar, Patrik Flodr, Jan Stritesky, Anna Jonasova, Jaroslav Cermak, Vladimir Divoky
Summary: The study sheds light on the molecular network complexity underlying response/resistance to hypomethylating agents in MDS and AML-MRC, suggesting that the metabolic status and transcriptional characteristics of CD34(+) cells may be pivotal factors in predicting patient responses.
Review
Oncology
Hana Votavova, Monika Belickova
Summary: The translated article discusses the differences between hypoplastic myelodysplastic syndrome (hMDS) and aplastic anemia (AA), highlighting the importance of molecular testing.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Oncology
Michaela Dostalova Merkerova, Jiri Klema, David Kundrat, Katarina Szikszai, Zdenek Krejcik, Andrea Hrustincova, Iva Trsova, Anh Vu Le, Jaroslav Cermak, Anna Jonasova, Monika Belickova
Summary: This study reveals that lncRNAs have the strongest predictive potential for AZA treatment. Through RNA sequencing analysis, a set of 14 lncRNAs and a few other genes and RNAs were identified as biomarkers for predicting the effectiveness of AZA treatment. The study also suggests the importance of epigenetic regulation and recombinational repair in AZA response.
CANCER GENOMICS & PROTEOMICS
(2022)
Article
Oncology
Monika Kaisrlikova, Jitka Vesela, David Kundrat, Hana Votavova, Michaela Dostalova Merkerova, Zdenek Krejcik, Vladimir Divoky, Marek Jedlicka, Jan Fric, Jiri Klema, Dana Mikulenkova, Marketa Stastna Markova, Marie Lauermannova, Jolana Mertova, Jacqueline Soukupova Maaloufova, Anna Jonasova, Jaroslav Cermak, Monika Belickova
Summary: This study found that the RUNX1 mutation is the main predictor of rapid progression in LR-MDS patients. Comparison of gene expression profiles between patients with and without RUNX1 mutations suggests that RUNX1 mutations may trigger malignant transformation. Dysregulation of DDR and cellular senescence was also observed. Additionally, the expression profiles of RUNX1-mutated LR-MDS resemble those of higher-risk MDS.