4.7 Article

Optimization of biodemulsifier production from Alcaligenes sp S-XJ-1 and its application in breaking crude oil emulsion

期刊

JOURNAL OF HAZARDOUS MATERIALS
卷 183, 期 1-3, 页码 466-473

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2010.07.047

关键词

Biodemulsifier; Crude oil emulsion; Oilfield produced water; Cultivation conditions; Optimization

资金

  1. National Natural Science Foundation of China [50908166]
  2. Shanghai Tongji Gao Tingyao Environmental Science and Technology Development Foundation

向作者/读者索取更多资源

A biodemulsifier-producing strain of Alcaligenes sp. S-XJ-1, isolated from petroleum-contaminated soil of the Karamay Oilfield, exhibited excellent demulsifying ability. The application of this biodemulsifier significantly improved the quality of separated water compared with the chemical demulsifier, polyether, which clearly indicates that it has potential applications in the crude oil extraction industry. To optimize its biosynthesis, the impacts of carbon sources, nitrogen sources and pH were studied in detail. Paraffin, a hydrophobic carbon source, favored the synthesis of this cell wall associated biodemulsifier. The nitrogen source ammonium citrate stimulated the production and demulsifying performance of the biodemulsifier. An alkaline environment (pH 9.5) of the initial culture medium favored the strain's growth and improved its demulsifying ability. The results showed paraffin, ammonium citrate and pH had significant effects on the production of the biodemulsifier. These three variables were further investigated using a response surface methodology based on a central composite design to optimize the biodemulsifier yield. The optimal yield conditions were found at a paraffin concentration of 4.01%, an ammonium citrate concentration of 8.08 g/L and a pH of 9.35. Under optimal conditions, the biodemulsifier yield from Alcaligenes sp. S-XJ-1 was increased to 3.42 g/L. (C) 2010 Elsevier B.V. All rights reserved.

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