期刊
JOURNAL OF GENETICS AND GENOMICS
卷 38, 期 2, 页码 89-94出版社
SCIENCE PRESS
DOI: 10.1016/j.jcg.2011.01.004
关键词
Allopolyploid; Doubled haploid; Unreduced gametes
资金
- National Basic Research Program (973 Program) of China [2009CB118300]
- National Natural Science Foundation of China [31071420]
- Doctoral Fund [2.00951E+13]
- CAS
- Education Commission and Science and Technology Commission of Sichuan Province
- South Dakota Agricultural Experiment Station
Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific F(1)s. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticum aestivum L.) and molecular markers were used to demonstrate the SynDH method.
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