Article
Fisheries
Feng Hu, Yingying Li, Qing Wang, Bin Zhu, Siyu Wu, Yingying Wang, Weiwei Zeng, Jiyuan Yin, Chun Liu, Sven M. Bergmann, Cunbin Shi
Summary: The study demonstrates that the DNA vaccine system based on SWCNTs can effectively combat KHV, with high concentration SWCNTs-p149 inducing stronger and longer-lasting immune responses compared to p149 alone. The vaccine also provided a high level of protection against KHV in juvenile koi fish. This research offers a new approach for controlling KHV in koi fish populations.
Article
Virology
Min Qian, Simin Xiao, Yapeng Yang, Fei Yu, Jinxuan Wen, Liqun Lu, Hao Wang
Summary: This study investigated the biological function of the open reading frame 55 (ORF55) gene of Cyprinid herpesvirus 2 (CyHV-2) in viral replication. The purified CyHV-2 ORF55 protein was obtained using prokaryotic expression, and interacting peptides were identified through phage display. Host interacting proteins were predicted and validated.
Article
Fisheries
Sven Michael Bergmann, Juliane Dabels, Sandro Klafack, Yeonwha Jin, Pei-Yu Lee, Arndt Christian Hofmann, Yingying Wang, Qing Wang, Yingying Li, Weiwei Zeng, Angela Lusiastuti, Shucheng Zheng, Yijuan Jin, Jolanta Kielpinska, Sean Monaghan
Summary: The study found that experimentally infected rainbow trout could transmit KHV to naive carp at different temperatures, and rainbow trout developed humoral response against KHV at both temperatures. The antibodies produced by rainbow trout in response to KHV differed from those observed in carp.
JOURNAL OF FISH DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Zhuoyu Wang, Nan Zheng, Jie Liang, Qiuju Wang, Xiujie Zu, Hao Wang, Haiyan Yuan, Ruixue Zhang, Shanshan Guo, Yanhui Liu, Jingxiang Zhou
Summary: The study found that emodin can effectively suppress the replication of CyHV-3, increase the survival rate of koi, and protect against the adverse effects induced by CyHV-3 by enhancing antioxidant function and reducing oxidative stress and inflammation cytokines expression.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Fisheries
Allahbagash Badhusha, Abdul Nafeez Ahmed, Selvam Suryakodi, Mohamed Jaffer Abdul Wazith, Sivaraj Mithra, Kumarasamy Kanimozhi, Seepoo Abdul Majeed, Gani Taju, Azeez Sait Sahul Hameed
Summary: This study reports the occurrence of Cyprinid herpesvirus 3 (CyHV-3) in koi carp for the first time in India. PCR assay and sequence analysis confirmed the presence of CyHV-3 in infected fish samples. CyHV-3 was found to replicate in cell lines of koi carp and catla, and tissue tropism analysis revealed its presence in vital organs with prominent band in gill and gut tissue.
JOURNAL OF FISH DISEASES
(2022)
Article
Virology
Andrea Krieter, Huai Xu, Haji Akbar, Taejoong Kim, Keith William Jarosinski
Summary: The conserved herpesvirus protein kinase (CHPK) has been identified as essential for the horizontal transmission of Marek's disease virus (MDV). This study confirms the importance of CHPK activity in the horizontal spread of Gallid alphaherpesvirus 3 (GaHV3) and suggests that avian CHPKs play a conserved role in natural infection.
Article
Microbiology
Mengfang Yang, Yuzhou Jiao, Yuanyuan Yan, Lisha Li, Xiaoshuai Hu, Zhe Jiao, Mengxia Li, Yixi Chen, Yuejun Shi, Zhou Shen, Guiqing Peng
Summary: Feline herpesvirus-1 (FHV-1) is a common causative agent of feline viral rhinotracheitis. Current commercial FHV-1 vaccines have safety concerns due to the retention of virulent genes. A novel recombinant FHV-1 (WH2020?TK/gI/gE) was constructed through CRISPR/Cas9-mediated homologous recombination, which showed reduced pathogenicity and provided better protection against FHV-1 challenge compared to the commercial vaccine.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Mikolaj Adamek, Marek Matras, Alexander Rebl, Magdalena Stachnik, Alberto Falco, Julia Bauer, Anne-Carina Miebach, Felix Teitge, Verena Jung-Schroers, Muhammad Abdullah, Torben Krebs, Lars Schroeder, Walter Fuchs, Michal Reichert, Dieter Steinhagen
Summary: Vaccination is the best way to protect fish from viral diseases. Live attenuated virus vaccines have shown to be most effective. This study demonstrates that vaccination with a live attenuated virus can enhance fish immune responses and protect against severe disease caused by a specific pathogen.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Isaiah E. Tolo, Przemyslaw G. Bajer, Sunil K. Mor, Nicholas B. D. Phelps
Summary: Cyprinid herpesvirus-3 (CyHV-3) is a significant pathogen that affects common carp populations worldwide. A study conducted in Minnesota revealed low prevalence of CyHV-3 in native fish species but infection rates ranging from 10% to 50% in carp populations across five lakes. Further investigations in Lake Elysian showed no positive detections of CyHV-3 in fish tissues, although the virus was detected in carp tissues, suggesting a potential latency site in the brain. The study also found that young carp, particularly males, were most impacted by CyHV-3-associated mortality and acute infections.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Friederike Eilts, Lisa K. Jordan, Yasmina M. J. Harsy, Sven M. Bergmann, Anna M. Becker, Michael W. Wolff
Summary: In this study, a purification method for infectious KHV was evaluated using steric exclusion chromatography to remove host cell protein and DNA. The method achieved high recoveries and impurity removal, with a yield of up to 55% using 12% PEG and 3-5 μm cellulose membranes. Additionally, >0.6 M NaCl was shown to inactivate infectious KHV.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Fengjun Liu, Shaoping Weng, Jianguo He, Chuanfu Dong
Summary: This study revealed the involvement of CypA protein in CyHV-3 replication, as it interacts with the viral envelope protein VP32. Inhibition of CypA reduces viral proliferation, while overexpression enhances it.
Article
Fisheries
Yihua Zheng, Yi Zhou, Lupin Zhao, Jiaxun Li, Liqun Lu, Yousheng Jiang
Summary: CyHV-3 is the main pathogen causing koi herpesvirus disease. A lateral flow immuno-chromatographic assay (LFIA) using specific monoclonal antibodies has been developed for on-site detection of CyHV-3. The LFIA strip showed a low detection limit and high specificity, making it an effective device for early detection of CyHV-3 in the future.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Z. H. Fan, Y. Xu, W. Luo, X. C. He, T. T. Zheng, J. J. Zhang, X. Y. Xu, Q. W. Qin, X. Z. Lee
Summary: In this study, ccCD63 was cloned from common carp and found to be expressed in various tissues with higher levels in spleen and brain. After KHV infection, the expression levels of ccCD63 varied in different tissues, with knockdown of ccCD63 promoting KHV infection. ccCD63 may be involved in antiviral response through the RIG-I signaling pathway.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Isaiah E. Tolo, Przemyslaw G. Bajer, Tiffany M. Wolf, Sunil K. Mor, Nicholas B. D. Phelps
Summary: This article investigates the transmission mechanisms and factors influencing the spread of Cyprinid herpesvirus 3 (CyHV-3) in fish populations. The study shows that direct contact is the primary transmission route for CyHV-3, and transmission mainly occurs during the incubation period of the disease. Furthermore, potential virulence-transmission tradeoffs and disease avoidance behaviors relevant to CyHV-3 transmission were identified.
Article
Fisheries
Yaojia Zhou, Ping Ouyang, Yu Tao, Lizi Yin, Kaiyu Wang, Yi Geng, Weiming Lai, Defang Chen, Hongrui Guo, Jing Fang, Zhengli Chen, Li Tang, Xiaoli Huang
Summary: This study revealed the regulatory role of CyHV-3 vTNFRs in host immune response and cell apoptosis, indicating that vTNFRs participate in virus invasion by up-regulating the expression levels of certain immune-related factors and inducing cell apoptosis.
AQUACULTURE REPORTS
(2021)
Article
Microbiology
Sandro Klafack, Qing Wang, Weiwei Zeng, Yingying Wang, Yingying Li, Shucheng Zheng, Jolanta Kempter, Pei-Yu Lee, Marek Matras, Sven M. Bergmann
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Virology
Lars Schroeder, Sandro Klafack, Sven M. Bergmann, Pei-Yu Alison Lee, Kati Franzke, Dirk Hoeper, Thomas C. Mettenleiter, Walter Fuchs
Article
Virology
Sandro Klafack, Anna-Sophie Fiston-Lavier, Sven M. Bergmann, Saliha Hammoumi, Lars Schroeder, Walter Fuchs, Angela Lusiastuti, Pei-Yu Lee, Sarahi Vega Heredia, Anne-Sophie Gosselin-Grenet, Jean-Christophe Avarre, Charles Amoyal, Hajar Amraoui, Audrey Bigourdan, Ines Boujandir, Oeane Bueno, Betty Cotteux, Noemie de San Nicolas, Romain Delattre, Mame Boucar Diouf, Remy Dussaut, Kevin Gawron, Jean-Baptiste Imbert, Halima Mouilhi, Emilie Nifaut, Anais Pages, Lucien Platon, Sandrine Sanchez, Lena Simon, Helene Sobry, Mathilde Souche, Boris Taillefer, Edith Ndiesseu Guemgne, Olivier Sacchi, Amad Diouf, Amirouche Labib Ouzerdine, Faustine Durand, Julie Brooke, Julie Cremaschi, Mathieu Massaviol, Mickael Hamouma, Mohammad Salma, Morgan Soulie, Quentin Delorme, Thimothee Virgoulay, Valentin Klein, Yannick Antoine, Aurore Berne, Camille Gaal
Article
Genetics & Heredity
Philippine Garret, Frederic Ebstein, Geoffroy Delplancq, Blandine Dozieres-Puyravel, Aicha Boughalem, Stephane Auvin, Yannis Duffourd, Sandro Klafack, Barbara A. Zieba, Sana Mahmoudi, Karun K. Singh, Laurence Duplomb, Christel Thauvin-Robinet, Jean-Marc Costa, Elke Krueger, Detlef Trost, Alain Verloes, Laurence Faivre, Antonio Vitobello
Article
Biotechnology & Applied Microbiology
S. M. Bergmann, Y. Jin, K. Franzke, B. Grunow, Q. Wang, S. Klafack
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Gonca cetin, Sandro Klafack, Maja Studencka-Turski, Elke Kruger, Frederic Ebstein
Summary: The ubiquitin-proteasome system (UPS) is a crucial protein degradation system in cells, particularly impacting immune cells and their rapid functional remodeling during immune activation. Recent research has shed light on the molecular mechanisms by which UPS contributes to immune responses and its role in immune disorders like cancer and auto-inflammatory diseases.
Article
Fisheries
Sven Michael Bergmann, Juliane Dabels, Sandro Klafack, Yeonwha Jin, Pei-Yu Lee, Arndt Christian Hofmann, Yingying Wang, Qing Wang, Yingying Li, Weiwei Zeng, Angela Lusiastuti, Shucheng Zheng, Yijuan Jin, Jolanta Kielpinska, Sean Monaghan
Summary: The study found that experimentally infected rainbow trout could transmit KHV to naive carp at different temperatures, and rainbow trout developed humoral response against KHV at both temperatures. The antibodies produced by rainbow trout in response to KHV differed from those observed in carp.
JOURNAL OF FISH DISEASES
(2021)
Article
Immunology
Sandro Klafack, Lars Schroeder, Yeonhwa Jin, Matthias Lenk, Pei-Yu Lee, Walter Fuchs, Jean-Christophe Avarre, Sven M. Bergmann
Summary: In this study, a live attenuated vaccine virus was generated and shown to protect carp or koi against wild type challenge. Vaccination induced mild clinical signs and produced protecting antibodies.
Article
Fisheries
S. M. Bergmann, S. Klafack, Y. Jin, V Jung-Schroers, A. Kappe, E. Nardy, S. Bornstein, N. Scuda, J. Kilwinski, M. Runge, T. L. Pruefer, H-P Hamann, W- Bock, T. Siempelkamp, A. Engelhardt, A. Salditti, M. Alex, S. Semmelmann, H. Wonnemann, D. Steinhagen, D. Stone, K. Way, M. Adamek
BULLETIN OF THE EUROPEAN ASSOCIATION OF FISH PATHOLOGISTS
(2020)