期刊
JOURNAL OF GENERAL VIROLOGY
卷 95, 期 -, 页码 806-815出版社
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.061838-0
关键词
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资金
- National Basic Research Program of China [2011CB5047, 2012CB518904]
- National Natural Science Foundation of China [31170158, 31300151]
- '100 Talents Project' of Chinese Academy of Sciences, China [Y002171YC1]
A full-length genome infectious clone is a powerful tool for functional assays in virology. In this study, using a chemical synthesized complete genome Of Japanese encephalitis virus (JEV) strain SA14 (GenBank accession no. U14163), we constructed a full-length genomic cDNA clone of JEV. The recovered virus from the cDNA clone replicated poorly in baby hamster kidney (BHK-21) cells and in suckling mice brain. Following serial passage in BHK-21 cells, adaptive mutations within the NS2B and NS4A proteins were recovered in the passaged viruses leading to viruses with a large-plaque phenotype. Mutagenesis analysis, using a genome-length RNA and a replicon of JEV, demonstrated that the adaptive mutations restored replication to different degrees, and the restoration efficiencies were in the order: NS2B-T102M
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