Article
Immunology
Cao-Wei Chen, Lei Yuan, Wen-yuan Zhou, Fedrick C. Mgomi, Yuan-song Zhang, Yang Wang, Xiang-feng Zheng, Qin Hu, Lu Gao, Sheng-qi Rao, Zhen-quan Yang, Xin-an Jiao
Summary: A new virulent phage (P.A-5) infecting Enterobacter hormaechei was isolated, characterized, and found to completely inhibit the growth of the pathogen in pork. This suggests the potential use of P.A-5 as a biocontrol agent for E. hormaechei.
MICROBIAL PATHOGENESIS
(2021)
Article
Food Science & Technology
Ya-Ke Li, Hu Chen, Mei Shu, Chan Zhong, Yan Bi, Huan-Huan Yang, Guo-Ping Wu
Summary: The alkali-resistant phage JN01 showed lytic activities against multiple E. coli strains, including multidrug resistant strains. It exhibited stability under stressed conditions and its genome did not contain known virulence-associated or antibiotic-related genes. Treatment with JN01 reduced viable cell counts of E. coli O157:H7 in milk and raw beef samples, suggesting its potential as a biological control agent for E. coli O157:H7 in food.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Georgi Merhi, Sara Amayri, Ibrahim Bitar, George F. Araj, Sima Tokajian
Summary: This study aimed to characterize multidrug resistant Enterobacter spp. and K. aerogenes isolates recovered from hospitalized patients in Lebanon. The isolates exhibited various resistance mechanisms and plasmid profiles. Multilocus sequence typing revealed the diversity within the isolates. Screening and continuous monitoring can effectively limit the dissemination of drug-resistant isolates in hospitalized patients.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Hyerim Kwak, Jinshil Kim, Sangryeol Ryu, Jaewoo Bai
Summary: Phage KMSP1, isolated from raw milk, was characterized as a lytic phage belonging to the Myoviridae family. It showed efficient antimicrobial activity against a broad spectrum of S. aureus strains, including MRSA. The presence of putative tail lysin with antimicrobial activity and the stability of KMSP1 under various conditions suggested its potential as a biocontrol agent.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wen-Yuan Zhou, Si-Fan Sun, Yuan-Song Zhang, Qin Hu, Xiang-Feng Zheng, Zhen-Quan Yang, Xin-An Jiao
Summary: The study found that a bacteriophage SapYZU01 can effectively control the growth of Salmonella Enteritidis in mixed-ingredient salads, reducing bacterial levels significantly and showing promising effects on lettuce and chicken meat salads. The results indicate the potential application of SapYZU01 as a natural biocontrol agent in mixed-ingredient salads, although the MOI and treatment method need to be considered.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Environmental Sciences
Tarek H. M. Elsharouny, Moustafa E. Shalaby, Nagwa M. Elkhateeb, Ali A. A. Hafez
Summary: Ten bacteriophages specific for Bacillus megaterium were isolated from rhizosphere soil of wheat plants, with each group sharing similar characteristics.
FRESENIUS ENVIRONMENTAL BULLETIN
(2022)
Article
Fisheries
Wei Lin, Dengfeng Li, Mingming Gao, Weinan Qin, Lihua Xu, Lingting Pan, Wencai Liu, Hang Fan, Zhiqiang Mi, Yigang Tong
Summary: Enterobacter hormaechei, an emerging pathogen, was found to be lethal to fish. A lytic phage IME523 infecting multidrug-resistant E. hormaechei was isolated from hospital sewage, showing potential therapeutic value.
DISEASES OF AQUATIC ORGANISMS
(2021)
Article
Microbiology
Yuting Shang, Qifan Sun, Hanfang Chen, Qingping Wu, Moutong Chen, Shuanghong Yang, Mingzhu Du, Fei Zha, Qinghua Ye, Jumei Zhang
Summary: The phage vB_SalP_TR2 showed high lytic potential against Salmonella serovar Albany and other tested serovars, it also exhibited tolerance to high temperature and pH. Additionally, treatment with phage vB_SalP_TR2 reduced the number of Salmonella in food matrices, highlighting its potential as an antibacterial agent for controlling Salmonella in food samples.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Qian Chen, Zhewen Dong, Tingting Ding, Qilin Yang, Chang Liu, Fang Yin, Huanlong Qin
Summary: In this study, a novel lytic Enterococcus gallinarum phage, Phi_Eg_SY1, with favorable thermostability and pH stability was isolated. It was found that Phi_Eg_SY1 can efficiently adsorb and lyse the host bacteria in vitro. Genomic and phylogenetic analyses revealed that Phi_Eg_SY1 does not contain virulence or lysogeny genes and represents a novel unassigned evolutionary lineage among related dsDNA phages. Therefore, Phi_Eg_SY1 is considered suitable for further applications.
Article
Immunology
Dongxiao Cheng, Fei Li, Jing Li, Shiyu Bai, Yong Wang, Xianfen Ma, Mengzhe Li, Xiaoping An, Lihua Song, Fei Ma, Bin Zhao, Yigang Tong
Summary: In this study, a new bacteriophage IME278 was isolated from hospital sewage in Beijing, China. It has the potential to be used as a new antibacterial strategy to control bacterial contamination in food. IME278 showed good stability under high temperatures (50-70 degrees Celsius) and a wide pH range (4.0-11.0).
MICROBIAL PATHOGENESIS
(2023)
Article
Multidisciplinary Sciences
Xuemei Duan, Liming Jiang, Ming Guo, Chenghua Li
Summary: In this study, a virulent phage vB_VspM_VS1, which infects the pathogenic bacterium V. splendidus, was isolated from aquaculture ponds in Dalian, China. The complete genome of vB_VspM_VS1 was characterized, and it was found to inhibit the growth and biofilm formation of V. splendidus.
Article
Virology
Jun Kwon, Sang Guen Kim, Hyoun Joong Kim, Sib Sankar Giri, Sang Wha Kim, Sung Bin Lee, Se Chang Park
Summary: The increasing emergence of antimicrobial resistance has led researchers to seek alternative antibiotics, with bacteriophage showing promise as one. A novel jumbo-phage isolated from Salmonella in exotic pet markets demonstrated the ability to inhibit bacterial growth and possess a wide host range. The genetic analysis revealed it belongs to Machinavirus and is composed of Erwnina phages, adding to the diversity of jumbo phages.
Article
Cell Biology
Xiaohu Cui, Bing Du, Junxia Feng, Yanling Feng, Zheng Fan, Jinfeng Chen, Jinghua Cui, Lin Gan, Tongtong Fu, Ziyan Tian, Rui Zhang, Chao Yan, Hanqing Zhao, Wenjian Xu, Ziying Xu, Zihui Yu, Zanbo Ding, Zhoufei Li, Yujie Chen, Guanhua Xue, Jing Yuan
Summary: This study isolated a novel lytic phage that effectively lysed multidrug-resistant K. aerogenes and relieved pneumonia caused by it. The phage carries a depolymerase that removes biofilms, reduces bacterial virulence, and enhances sensitivity to serum killing.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Chemistry, Applied
Mengshi Xiao, Xiaodan Fu, Xinyi Wei, Yongzhou Chi, Wei Gao, Ying Yu, Zhemin Liu, Changliang Zhu, Haijin Mou
Summary: This study successfully utilized bacteriophage-borne glycanase to degrade FcEPS from Enterobacter sakazakii and obtain disaccharide fragments of FCOs. The FcEPS was shown to be a reliable source of disaccharides, and bacteriophage-borne glycanase was an effective tool for degradation.
CARBOHYDRATE POLYMERS
(2021)
Article
Parasitology
Elahe Tajbakhsh, Shima Shantiae, Hassan Momtaz
Summary: This study investigated the mechanisms of resistance in Enterobacter aerogenes strains isolated from the urinary tract of infected patients. The findings indicate that E. aerogenes strains exhibit high levels of antibiotic resistance and biofilm formation, and are associated with various resistance and virulence genes.
