Article
Microbiology
Vesa Aho, Sami Salminen, Salla Mattola, Alka Gupta, Felix Flomm, Beate Sodeik, Jens B. Bosse, Maija Vihinen-Ranta
Summary: Herpes simplex virus capsids are assembled in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Changes in chromatin architecture during infection enhance nuclear diffusion of capsids at late infection, which is the rate-limiting step for the nuclear egress of the virus. Understanding the kinetics of capsid translocation in the nucleus is fundamental for DNA virus research.
Article
Microbiology
Ethan C. M. Thomas, Renee L. Finnen, Jeffrey D. Mewburn, Stephen L. Archer, Bruce W. Banfield
Summary: The expression of HSV pUL16 and pUL21 proteins can prevent nucleocapsids from binding to nuclear pore complexes and delivering their genomes back to the nucleus. This finding provides important insights into the biology of herpes simplex virus.
Article
Virology
Nabil El Bilali, Bita Khadivjam, Eric Bonneil, Pierre Thibault, Roger Lippe
Summary: The study investigated the composition of HSV-1 A, B, and C nuclear capsids, identifying host proteins specific to C-capsids. By purifying viral particles using a novel method, it aims to uncover the differences and functional relevance of these viral capsids. This innovative approach opens up new research avenues to clarify the biology of herpesviruses.
JOURNAL OF VIROLOGY
(2021)
Correction
Multidisciplinary Sciences
Rosamaria Pennisi, Maria Musarra-Pizzo, Zhixiang Lei, Grace Guoying Zhou, Maria Teresa Sciortino
Summary: The paper has been amended and the revised version can be accessed through a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Tomasz H. Benedyk, Julia Muenzner, Viv Connor, Yue Han, Katherine Brown, Kaveesha J. Wijesinghe, Yunhui Zhuang, Susanna Colaco, Guido A. Stoll, Owen S. Tutt, Stanislava Svobodova, Dmitri I. Svergun, Neil A. Bryant, Janet E. Deane, Andrew E. Firth, Cy M. Jeffries, Colin M. Crump, Stephen C. Graham
Summary: The HSV-1 protein pUL21 functions as an adaptor for protein phosphatase 1 (PP1), directing the dephosphorylation of cellular and viral proteins. By antagonizing the activity of the virus-encoded kinase pUS3, pUL21 plays a crucial role in balancing kinase and phosphatase activities for optimal virus replication and spread.
Article
Virology
Katinka Doehner, Angela Cornelius, Manutea Christophe Serrero, Beate Sodeik
Summary: This article highlights the key steps in herpesviral infection, including capsid stabilization during assembly, early interactions with host cells to ensure nuclear targeting, and genome uncoating. After fusion with a host membrane, capsids recruit microtubule motors for traveling to the centrosome, eventually reaching the nucleus. Capsid-associated tegument proteins interact with nucleoporins to facilitate injection of genomes into the nucleoplasm for transcription and replication, while some cell types may disarm or silence incoming capsids to prevent infection.
CURRENT OPINION IN VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomasz H. Benedyk, Viv Connor, Eve R. Caroe, Maria Shamin, Dmitri I. Svergun, Janet E. Deane, Cy M. Jeffries, Colin M. Crump, Stephen C. Graham
Summary: Herpes simplex virus-1 (HSV-1) alters cellular membrane lipid composition during infection. This study demonstrates that a virus-encoded protein, pUL21, promotes the conversion of ceramide (Cer) to sphingomyelin (SM) by activating CERT. The study also reveals the importance of specific protein-protein interactions in HSV-1 mediated sphingolipid metabolism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Microbiology
Kamal L. Nahas, Viv Connor, Katharina M. Scherer, Clemens F. Kaminski, Maria Harkiolaki, Colin M. Crump, Stephen C. Graham
Summary: Herpes simplex virus-1 (HSV-1) infection causes morphological changes in cellular compartments, as well as specific organelles. These changes can be captured using soft X-ray tomography, which reveals the significant impact of HSV-1 infection on the morphology of cellular compartments.
Article
Biochemistry & Molecular Biology
Katharina M. Scherer, James D. Manton, Timothy K. Soh, Luca Mascheroni, Vivienne Connor, Colin M. Crump, Clemens F. Kaminski
Summary: Herpesviruses, having evolved with host species, alter intracellular morphology during replication, crucial for viral assembly. A dual-fluorescent reporter virus method successfully tracked stages in HSV-1 infection cycle, revealing spatial and temporal reorganization of cellular structures during viral replication. The study suggests similar tools could be applied for systems-level analysis of intracellular morphology in replication of other viruses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Bita Khadivjam, Eric Bonneil, Pierre Thibault, Roger Lippe
Summary: DDX3X is a mammalian RNA helicase that plays multiple roles in regulating RNA metabolism, cancers, innate immunity, and RNA viruses. This study reveals that herpes simplex virus 1 can redirect DDX3X to the nuclear envelope, where it modulates the exit of newly assembled viral particles. The depletion of DDX3X leads to the accumulation of virions in intranuclear herniations. Mechanistically, DDX3X interacts with the viral nuclear egress complex and stimulates the incorporation of pUS3, a viral kinase, in mature viral particles, thereby facilitating viral nuclear release. This research unveils the complex interaction between DDX3X and herpes simplex viruses, providing insights into viral propagation and potential targets for therapeutic intervention.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Elizabeth B. Draganova, Michael K. Thorsen, Ekaterina E. Heldwein
Summary: During viral replication, herpesviruses use a unique strategy called nuclear egress to translocate capsids from the nucleus to the cytoplasm, involving multiple budding and fusion steps. The nuclear egress complex (NEC), consisting of viral proteins UL31 and UL34, plays a crucial role in mediating this process. Other viral proteins may be involved in regulating NEC-mediated budding during infection.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
Article
Ophthalmology
Paul J. Steptoe, Catherine M. Guly, Andrew D. Dick
Summary: This study compares the cross-sectional retinal lesion appearance in patients with punctate inner choroidopathy (PIC), Ebola virus disease (EVD) retinopathy, and a mouse model of herpes simplex virus type I (HSV) retinopathy. The results show a similar lesion appearance on optical coherence tomography in both PIC and EVD retinopathy, with focal photoreceptor loss and distinctive V-shaped collapse of the overlying retinal layers. This similarity suggests a potential common pathophysiology and calls for further research.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Allergy
Mohammad Ali Khodadoust, Gholam Reza Keramatian, Najmeh Kaffash Farkhad, Jalil Tavakol-Afshari
Summary: The study shows that Herbix at a dose of 300 mg/mL is effective in treating murine herpes and stimulating immune responses, making it a potential candidate for further investigation as an antiherpetic drug.
IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nathalie Scrima, Jean Lepault, Yves Boulard, David Pasdeloup, Stephane Bressanelli, Stephane Roche
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Virology
Frauke Beilstein, Linda Obiang, Helene Raux, Yves Gaudin
JOURNAL OF VIROLOGY
(2015)
Review
Oncology
Frauke Beilstein, Veronique Carriere, Armelle Leturque, Sylvie Demignot
EXPERIMENTAL CELL RESEARCH
(2016)
Article
Gastroenterology & Hepatology
Frauke Beilstein, Matthieu Lemasson, Veronique Pene, Dominique Rainteau, Sylvie Demignot, Arielle R. Rosenberg
Article
Virology
Frauke Beilstein, Matthieu Blanchet, Andrew Vaillant, Camille Sureau
JOURNAL OF VIROLOGY
(2018)
Review
Biochemistry & Molecular Biology
Sylvie Demignot, Frauke Beilstein, Etienne Morel
Article
Virology
David Pasdeloup, Marion McElwee, Frauke Beilstein, Marc Labetoulle, Frazer J. Rixon
JOURNAL OF VIROLOGY
(2013)
Article
Multidisciplinary Sciences
Frauke Beilstein, Julien Bouchoux, Monique Rousset, Sylvie Demignot
Article
Microbiology
Frauke Beilstein, Gary H. Cohen, Roselyn J. Eisenberg, Valerie Nicolas, Audrey Esclatine, David Pasdeloup
Article
Virology
Aurelien Chuard, Katia Courvoisier-Guyader, Sylvie Remy, Stephen Spatz, Caroline Denesvre, David Pasdeloup
Summary: This study focuses on the role of tegument proteins pUL47 and pUL48 in the life cycle of Marek's disease virus (MDV), an alphaherpesvirus of chickens. The results show that pUL47 is crucial for viral transmission and the splicing and expression of UL44 gene encoding glycoprotein gC. Host-to-host transmission of MDV in chickens relies on the presence and function of pUL47, shedding light on potential similar functions in human herpesviruses.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Frauke Beilstein, Abbas Abou Hamdan, Helene Raux, Laura Belot, Malika Ouldali, Aurelie A. Albertini, Yves Gaudin
Article
Microbiology
Isabelle Lantier, Corentin Mallet, Laurent Souci, Thibaut Larcher, Andele M. Conradie, Katia Courvoisier, Sascha Trapp, David Pasdeloup, Benedikt B. Kaufer, Caroline Denesvre
Summary: In vivo bioluminescence imaging technique allows non-invasive visualization of biological processes in living animals. This study used a recombinant Marek's disease virus (MDV) expressing firefly luciferase to visualize the infection in chickens, leading to the discovery of novel sites of MDV replication.
Article
Virology
David Pasdeloup, Aurelien Chuard, Sylvie Remy, Katia Courvoisier-Guyader, Caroline Denesvre
Summary: Viruses spread through cell-released virus and/or cell-to-cell spread, but the molecular determinants and importance of cell-to-cell spread in viral infection are unclear. This study reveals that pUL51 is essential for the growth of Marek's disease virus, an oncogenic alphaherpesvirus of chickens that is strictly cell-associated. The study also shows the association of pUL51 with lipid droplets and the important role of its C-terminal half in viral replication and pathogenesis.
JOURNAL OF VIROLOGY
(2023)