Article
Neurosciences
Haoran Lin, Wenlu Li, Zexu Shen, Yun Bei, Taofeng Wei, Zhanyang Yu, Yunjian Dai, Haibin Dai
Summary: Promoting angiogenesis through ANXA2-A2R interaction can improve neurological outcomes after stroke. ANXA2 is involved in regulating microvessel density and new vessel sprouting, as well as promoting cell tube-numbers and migration in brain endothelial cells. Further investigation of ANXA2-A2R may lead to new therapeutic targets for stroke.
NEUROSCIENCE LETTERS
(2023)
Article
Oncology
Lee D. Gibbs, Kelsey Mansheim, Sayantan Maji, Rajesh Nandy, Cheryl M. Lewis, Jamboor K. Vishwanatha, Pankaj Chaudhary
Summary: Annexin A2 (AnxA2) is overexpressed in tumor tissues and serum samples of breast cancer patients, especially in the TNBC subtype, where its expression is associated with poor prognosis. The phosphorylation of AnxA2 at tyrosine 23 appears to be a critical step for its secretion from TNBC cells and could serve as a potential diagnostic biomarker in TNBC patients.
Article
Immunology
Michal Zygarowicz, Joanna Kacperczyk-Bartnik, Janusz Sierdzinski, Cezary Wojtyla, Piotr Pierzynski, Grzegorz Manka, Mariusz Kiecka, Robert Z. Spaczynski, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokita, Jakub Mlodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Michal Lipa, Damian Warzecha, Miroslaw Wielgos, Ewa Koc-Zorawska, Marcin Zorawski, Piotr Laudanski
Summary: This study investigated and compared the concentration of the protein annexin A2 (ANXA2) in plasma and peritoneal fluid in women with endometriosis compared to controls. The results showed that women with endometriosis had significantly higher levels of ANXA2 in their plasma compared to women without endometriosis. However, there were no significant differences in ANXA2 levels in the peritoneal fluid. Additionally, plasma concentrations of ANXA2 were significantly higher in patients with stage III and IV endometriosis compared to controls. Further studies are needed to confirm the role of ANXA2 in the pathogenesis of endometriosis.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Ophthalmology
Valentina Dallacasagrande, Wei Liu, Dena Almeida, Min Luo, Katherine A. Hajjar
Summary: The role of annexin A2 (A2) in the development of diabetic retinal vasculopathy was examined in this study. Anxa2 gene deletion and administration of anti-A2 antibodies were found to prevent pericyte dropout in diabetic mice and reduce vasoobliteration and neovascularization in a mouse model of oxygen-induced retinopathy. The combination of anti-A2 antibodies and anti-VEGF therapy showed an amplified effect.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Ann Kari Grindheim, Sudarshan S. Patil, Canan G. Nebigil, Laurent Desaubry, Anni Vedeler
Summary: Annexin A2 (AnxA2) plays a critical role in cell transformation, immune response, and resistance to cancer therapy. It acts as a calcium- and lipid-binding protein as well as an mRNA-binding protein, involved in the regulation of certain cytoskeleton-associated mRNAs. A new inhibitor called FL3 can transiently increase the expression of AnxA2 and stimulate short-term transcription/translation of anxA2 mRNA. AnxA2 regulates its own mRNA translation via a feedback mechanism, which can be partially relieved by FL3.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Alamelu Bharadwaj, Emma Kempster, David Morton Waisman
Summary: Mutualistic symbiosis refers to a symbiotic relationship between individuals of different species where both benefit. Annexin A2 and S100A10 have a mutualistic symbiosis where their interaction affects the function of both proteins. They rely on each other for certain functions and processes that benefit both.
Review
Biochemistry & Molecular Biology
Prakash Patil, Praveenkumar Shetty, Nithin Kuriakose, Pavan Gollapalli, Sukanya Shetty, Roopa Bhandary, Jamboor K. Vishwanatha, Sudeep D. Ghate
Summary: SARS-CoV-2 has caused infections and deaths worldwide, with millions affected and no clinically proven antivirals available. Efforts to develop effective drugs are being delayed by mutations and lack of understanding of viral mechanisms. AnxA2 may play a potential role in SARS-CoV-2 pathogenesis and post-infection complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Urology & Nephrology
Juan Lei, Pingping Sun, Jingyi Sheng, Hongri Wang, Yifan Xie, Jiayu Song
Summary: Annexin A2 (Anxa2) is a calcium-regulated phospholipid binding protein that plays important roles in acute kidney injury, chronic kidney disease, renal cell carcinoma differentiation, and the formation of nephrolithiasis.
Article
Cell Biology
Xin Li, Shengdan Nie, Ziyang Lv, Lingran Ma, Yuxi Song, Zhongxu Hu, Xin Hu, Zhiqiang Liu, Gaoya Zhou, Zhijie Dai, Tao Song, Jiajia Liu, Shan Wang
Summary: The study demonstrates that ANXA2 promotes the proliferation of glioma cells by forming a positive feedback loop with GPC1, and the combined overexpression of ANXA2 and GPC1 may indicate poor prognosis in glioma patients.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Hana Lim, Katherine A. Hajjar
Summary: Annexin A2 complex plays a crucial role in hemostasis and fibrinolysis on endothelial cell surface; Aberrant expression of A2 is associated with various diseases including thrombosis and inflammation; Dysregulation of A2 function may impact wound healing and tissue fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Amira F. Mahdi, Joanne Nolan, Ruth i. O'Connor, Aoife J. Lowery, Joanna M. Allardyce, Patrick A. Kiely, Kieran McGourty
Summary: The extracellular matrix (ECM) plays a significant role in the development and progression of breast cancer, and Annexin A2 has been identified as a key protein in this process. This study demonstrates that the presence of collagen-I in the ECM leads to the phosphorylation and translocation of Annexin A2, and identifies fibronectin as a novel interactor of Annexin A2. Furthermore, reducing Annexin A2 expression decreases the degradation of fibronectin by cancer cells, with this effect being influenced by collagen-I levels.
FRONTIERS IN ONCOLOGY
(2023)
Review
Oncology
Zinan Li, Lifeng Yu, Baohui Hu, Lianze Chen, Mingyi Jv, Lin Wang, Chenyi Zhou, Minjie Wei, Lin Zhao
Summary: ANXA2, a calcium regulated phospholipid-binding protein, is expressed in tumor cells, endothelial cells, and other cells, playing important roles in cell survival and interactions. Targeting ANXA2 in cancer cells has shown promising results and it can be used as a potential predictive factor and therapeutic target in cancer therapy.
Article
Cell Biology
Raffaella Belvedere, Elva Morretta, Emanuela Pessolano, Nunzia Novizio, Alessandra Tosco, Amalia Porta, James Whiteford, Mauro Perretti, Amelia Filippelli, Maria Chiara Monti, Antonello Petrella
Summary: Mesoglycan induces the release of plasmin in endothelial cells by triggering a series of events starting from SDC4 activation, followed by src complex and PKC alpha activation, ultimately leading to the phosphorylation of ANXA2 and its translocation to the plasma membrane. This cascade mechanism connects mesoglycan with SDC4-(PKC alpha-src) and ANXA2, ultimately facilitating the release of plasmin and degradation of fibrin sleeves.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu-Shi Wang, Chen Chen, Shi-Yin Zhang, Yang Li, Ying-Hua Jin
Summary: STAT3 is a crucial player in tumor development, and (20S)G-Rh2 may serve as a potential option for targeted therapy by inhibiting STAT3 activity through interfering with Annexin A2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Esraa Magdy Abdelraouf, Raghda R. S. Hussein, Ahmed Hassan Shaaban, Hany A. M. El-Sherief, Azza S. Embaby, Seham A. Abd El-Aleem
Summary: This study demonstrates the correlation between AnxA2, the proposed prognostic marker, and the well-established tumor markers HER2 and Ki67 in breast cancer. AnxA2 may play a role in breast cancer heterogeneity and poor prognosis. Additionally, there is a strong correlation between tissue and serum AnxA2, suggesting that assessing serum AnxA2 can be a noninvasive prognostic tool.
Article
Infectious Diseases
Gonzalo Lopez-Lorenzo, Cynthia Lopez-Novo, Alberto Prieto, Jose Manuel Diaz, Julian Gullon, Jose Luis Arnal, Alfredo Benito, Pablo Diaz, Rosario Panadero, Pablo Diez-Banos, Kevin P. Dalton, Francisco Parra, Gonzalo Fernandez
Summary: The study found that MYXV was detected in the environment of infected farms, while samples from non-infected farms were negative. All positive samples contained viral DNA compatible with field strains. Current vaccination schemes alone are not sufficient to prevent the disease and should be accompanied by adequate biosecurity measures.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Infectious Diseases
Fabio A. Abade dos Santos, Andreia Pinto, Thomas Burgoyne, Kevin P. Dalton, Carina L. Carvalho, David W. Ramilo, Carla Carneiro, Tania Carvalho, M. Conceicao Peleteiro, Francisco Parra, Margarida D. Duarte
Summary: The study confirms the presence and replication of RHDV2 in Eurasian badgers through various evidences, indicating their susceptibility to the virus and potential transmission to wild rabbits. This discovery suggests a paradigm shift in the replication cycle of caliciviruses.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Fabio A. Abade dos Santos, Carina L. Carvalho, Maria C. Peleteiro, Francisco Parra, Margarida D. Duarte
Summary: LeHV-5, the first herpesvirus in the genus Lepus, poses a threat to the already fragile populations of Iberian hare and requires further investigation. A quantitative molecular method for LeHV-5 detection has been described in this article, showing excellent sensitivity and specificity.
Article
Immunology
Kevin P. Dalton, Carmen Alvarado, Edel Reytor, Maria Del Carmen Nunez, Ana Podadera, Diego Martinez-Alonso, Jose Manuel Martin Alonso, Ines Nicieza, Silvia Gomez-Sebastian, Romy M. Dalton, Francisco Parra, Jose M. Escribano
Summary: The VP60 capsid protein from rabbit haemorrhagic disease virus can form virus-like particles when expressed using different protein expression systems. In this study, a dual-serving RHDV vaccine was successfully developed using CrisBio technology, which allows the co-expression of VP60 proteins from two RHDV serotypes. Rabbits immunised with the chimeric VLPs were fully protected against a lethal challenge infection with the two RHDV serotypes, demonstrating the effectiveness of the dual vaccine strategy.
Article
Biochemistry & Molecular Biology
Fabio A. Abade dos Santos, Carina L. Carvalho, Francisco Parra, Kevin P. Dalton, Maria C. Peleteiro, Margarida D. Duarte
Summary: A new recombinant myxoma virus, ha-MYXV, emerged in Iberian hare and European rabbit, with genetic differences from classic MYXV strains. A robust qPCR multiplex technique was developed to distinguish ha-MYXV from classic MYXV strains, allowing for fast and efficient differential diagnosis and monitoring of the geographical spread of ha-MYXV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Analytical
Elena Sanchez-Bascones, Francisco Parra, Maria Jesus Lobo-Casta
Summary: Aptamers, short single-stranded nucleic acid molecules selected through SELEX, show advantages over antibodies in viral detection. However, challenges such as low commercial availability and lack of standardized protocols limit their application. Various approaches for selecting aptamers against viruses and their potential use in optical, electrochemical, and piezoelectric transduction are discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Infectious Diseases
Fabio A. Abade dos Santos, Kevin P. Dalton, Carina L. Carvalho, Maria Casero, Angel L. Alvarez, F. Parra, Margarida D. Duarte
Summary: Myxomatosis, caused by ha-myxoma virus (MYXV), has been identified as an emergent disease in Iberian hare. Co-infection cases of classic MYXV and ha-MYXV strains were detected in one Iberian hare and one European wild rabbit. This is the first report of such co-infection in these species, highlighting the continuous evolution of MYXV and the importance of monitoring the health status of wild Leporidae populations in the Iberian Peninsula.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Ana Agueda-Pinto, Simona Kraberger, Anne Everts, Ami Gutierrez-Jensen, Honor L. Glenn, Kevin P. Dalton, Ana Podadera, Francisco Parra, Monica Martinez-Haro, Jose Alberto Vinuelas, Arvind Varsani, Grant McFadden, Masmudur M. Rahman, Pedro J. Esteves
Summary: The M159 protein plays a critical role in determining the host specificity of MYXV-Tol in hare and human cells by imparting new host range functions.
Article
Immunology
Fabio A. Abade dos Santos, Carina L. Carvalho, Pamela C. L. G. Valente, Henrique Armes, Sylvia S. Reemers, Maria C. Peleteiro, Ines Calonge Sanz, Kevin P. Dalton, Francisco Parra, Margarida D. Duarte
Summary: A new myxoma virus has caused outbreaks and population reduction in Iberian hares. The efficacy of commercial vaccines for domestic rabbits against this virus in Iberian hares and wild rabbits was evaluated. Higher doses of one vaccine showed potential protection for Iberian hares, while both vaccines tested were fully protective in wild rabbits.
Article
Microbiology
Angel L. Alvarez, Kevin P. Dalton, Ines Nicieza, Fabio A. Abade Dos Santos, Pilar de la Pena, Pedro Dominguez, Jose M. Martin-Alonso, Francisco Parra
Summary: Surface contamination plays an important role in the spread of SARS-CoV-2. Personal protective elements and physical barriers, such as masks, gloves, and indoor glass separators, increase safety and have proven effective in preventing contagion. This study explores the virucidal properties of a commercial TiO2-coated photocatalytic glass against a model human coronavirus, showing its potential to inactivate the virus and reduce transmission.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Mila M. Leuthold, Turgay Kilic, Jessica M. Devant, Oscar Landeta, Francisco Parra, Kevin P. Dalton, Grant S. Hansman
Summary: This study provides the structural basis for the specificity of an RHDV GI.2 diagnostic antibody (2D9) and explains how amino acid variation at the binding site restricts cross-reactivity. The findings have implications for characterizing antigenic variants and developing new monoclonal antibodies for diagnostics and therapeutics.
JOURNAL OF VIROLOGY
(2022)
Letter
Virology
Angel L. Alvarez, Francisco Parra
