期刊
JOURNAL OF GENERAL PHYSIOLOGY
卷 143, 期 1, 页码 119-134出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1085/jgp.201311063
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类别
资金
- National Institutes of Health [HL092286, HL113089, HL093368, DK092412, HL085820, HL070250, HL096652, HL079031, HL113001, AR052777, HL090905]
- VA Merit Review Program [1I0BX000718]
- Carver Trust [01-224]
- Fraternal Order of Eagles
ATP-sensitive potassium (K-ATP) channels have the unique ability to adjust membrane excitability and functions in accordance with the metabolic status of the cell. Skeletal muscles are primary sites of activity-related energy consumption and have K-ATP channels expressed in very high density. Previously, we demonstrated that transgenic mice with skeletal muscle-specific disruption of K-ATP channel function consume more energy than wild-type littermates. However, how K-ATP channel activation modulates skeletal muscle resting and action potentials under physiological conditions, particularly low-intensity workloads, and how this can be translated to muscle energy expenditure are yet to be determined. Here, we developed a technique that allows evaluation of skeletal muscle excitability in situ, with minimal disruption of the physiological environment. Isometric twitching of the tibialis anterior muscle at 1 Hz was used as a model of low-intensity physical activity in mice with normal and genetically disrupted K-ATP channel function. This workload was sufficient to induce K-ATP channel opening, resulting in membrane hyperpolarization as well as reduction in action potential overshoot and duration. Loss of K-ATP channel function resulted in increased calcium release and aggravated activity-induced heat production. Thus, this study identifies low-intensity workload as a trigger for opening skeletal muscle K-ATP channels and establishes that this coupling is important for regulation of myocyte function and thermogenesis. These mechanisms may provide a foundation for novel strategies to combat metabolic derangements when energy conservation or dissipation is required.
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