期刊
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
卷 64, 期 6, 页码 293-298出版社
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.2018.02.004
关键词
Aspergillus; cellulose; fungi; mangrove; mixed culture
资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA13020300]
- Guangdong Province Public Welfare Research and Capacity Building Project [2015A020216016]
- Science and Technology Planning Project of Guangdong Province, China [2014B030301064]
- Hainan Province Marine Science and Technology Special Project [ZH201415]
Fungi are the most suitable cellulase producers attributing to its ability to produce a complete cellulase system. 33 Genus, 175 Species fungi were isolated from Sanya mangrove, Hainan, China. Using congo red cellulose (CMC) medium, five fungi of cellulose-degrading were selected for further study. Molecular biology and morphological identification showed that all of these five fungi belong to Aspergillus fungi. The cellulase produced by these fungi were monitored during liquid state fermentation. The optimum conditions study for enzyme production illustrated that the highest activities appeared at pH 3.0, 35 degrees C after fermentation for 3 days. Beyond that, the enzyme activity of mixed fungi is 11-26% higher than pure. The study demonstrated that mixed culture improved the hydrolysis of fungi cellulase.
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