期刊
JOURNAL OF FORENSIC SCIENCES
卷 56, 期 -, 页码 S112-S122出版社
WILEY
DOI: 10.1111/j.1556-4029.2010.01632.x
关键词
forensic science; Lucilia sericata; Calliphoridae; RNA; generalized additive model; forensic entomology; gene expression; reverse transcription PCR
资金
- National Institute of Justice, Office of Justice Programs, US Department of Justice [2004-DN-BX-K005]
Forensic entomologists use size and developmental stage to estimate blow fly age, and from those, a postmortem interval. Since such estimates are generally accurate but often lack precision, particularly in the older developmental stages, alternative aging methods would be advantageous. Presented here is a means of incorporating developmentally regulated gene expression levels into traditional stage and size data, with a goal of more precisely estimating developmental age of immature Lucilia sericata. Generalized additive models of development showed improved statistical support compared to models that did not include gene expression data, resulting in an increase in estimate precision, especially for postfeeding third instars and pupae. The models were then used to make blind estimates of development for 86 immature L. sericata raised on rat carcasses. Overall, inclusion of gene expression data resulted in increased precision in aging blow flies.
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