Article
Nutrition & Dietetics
Yu Chen, Yu Zhang, Qianhui Qi, Feng Liang, Nan Wang, Qihe Chen, Xue Li, Suling Sun, Xinquan Wang, Kaiwen Bai, Wei Wang, Yingchun Jiao
Summary: The key factors affecting the ACE inhibition rate in mulberry leaf protein hydrolysate and the optimum conditions of enzymatic hydrolysis were determined. ACE-inhibitory peptides were purified from the hydrolysates and their interaction mechanisms with ACE were analyzed. The results showed that these peptides had potential activities to inhibit ACE and could be used as functional food or drugs.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
Jingjing Dong, Shen Wang, Xiaoyao Yin, Min Fang, Zhiyong Gong, Yongning Wu
Summary: This study investigated the antihypertensive effect of rice peptide (RP) and revealed its mechanism of action. The results demonstrated that RP could effectively reduce systolic blood pressure in hypertensive rats, potentially through regulating the renin-angiotensin system and nitric oxide release.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Review
Food Science & Technology
Nishithkumar Jogi, Undiganalu Gangadharappa Yathisha, Ishani Bhat, Bangera Sheshappa Mamatha
Summary: Food proteins are sources for ACE-I inhibitory peptides that can be extracted by enzymatic hydrolysis and exhibit anti-hypertensive activity. However, these peptides are susceptible to degradation by gastrointestinal enzymes during oral consumption. The bio-activity of ACE-I inhibitory peptides is dependent on their intact absorption after gastrointestinal digestion.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Agriculture, Multidisciplinary
Md Yousof Ali, Sumera Zaib, Susoma Jannat, Imtiaz Khan
Summary: Ginsenoside derivatives exhibit significant inhibitory effects on ACE, with protopanaxatriol, protopanaxadiol, and ginsenoside Rh2 showing the most potent inhibition. Molecular docking studies confirmed that ginsenosides inhibit ACE through hydrogen bonds and hydrophobic interactions. Additionally, ginsenosides also stimulate glucose uptake in insulin-resistant cells and have antioxidant properties.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Peripheral Vascular Disease
Ana R. Gaspar, Beatriz Andrade, Sara Mosca, Mariana Ferreira-Duarte, Ana Teixeira, Dina Cosme, Antonio Albino-Teixeira, Fernanda A. Ronchi, Ana P. Leite, Dulce E. Casarini, Jose C. Areias, Teresa Sousa, Alberto C. Afonso, Manuela Morato, Liane Correia-Costa
Summary: This study found that childhood obesity is associated with increased activities of angiotensin-converting enzymes (ACE) and ACE2. Overweight/obese children had significantly higher ACE and ACE2 activities compared to normal weight children. In girls, ACE and ACE2 activities were associated with increased night-time systolic and diastolic blood pressure.
JOURNAL OF HYPERTENSION
(2023)
Article
Food Science & Technology
Osama Magouz, Nabil Mehanna, Mohamed Khalifa, Hanaa Sakr, Sabrina Gensberger-Reigl, Sevim Dalabasmaz, Monika Pischetsrieder
Summary: A comprehensive analysis of the peptide fraction of fermented buttermilk was conducted using UHPLC-ESI-MS/MS before and after simulated gastrointestinal digestion. Results showed significant changes in the peptide profile of fermented buttermilk after digestion. A total of 81, 120, and 46 peptides were identified in fermented buttermilk, its gastric and intestinal digests, respectively. The bioactivity of fermented buttermilk peptides, mainly ACE inhibitory activity, increased significantly after digestion.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2023)
Article
Agriculture, Multidisciplinary
Carmen Lammi, Giovanna Boschin, Martina Bartolomei, Anna Arnoldi, Gianni Galaverna, Luca Dellafiora
Summary: This study combined computational and in vitro investigations to precisely describe the chemical basis of potent inhibitory peptides. A novel and potent ACE inhibitory peptide was discovered, and the findings could serve as a blueprint for designing new inhibitory peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Daniel Batista, Gabriela de Matuoka e Chiocchetti, Juliana Alves Macedo
Summary: This study aimed to produce high-phenolic-content extracts from red grape pomace and evaluate their antioxidant capacity and ACE inhibitory potential. The results showed that enzymatic extraction increased the extraction yield and ACE inhibitory potential, demonstrating the viability of upcycling grape pomace.
Article
Food Science & Technology
Marta Maria Calvo, Ana Belen Martin-Diana, Daniel Rico, Maria Elvira Lopez-Caballero, Oscar Martinez-Alvarez
Summary: This study found that a purified polyphenolic extract from the ice plant has strong antioxidant, antihypertensive, hypoglycaemic and nootropic activities. Flavones were identified as the major group responsible for the enzyme inhibition activity.
Article
Medicine, Research & Experimental
Mauro G. Silva, Gerardo R. Corradi, Juan Perez Duhalde, Myriam Nunez, Eliana M. Cela, Daniel H. Gonzales Maglio, Ana Brizzio, Martin R. Salazar, Walter G. Espeche, Mariela M. Gironacci
Summary: This study found that COVID-19 patients have higher levels of ACE2 expression and enzymatic activity in their blood compared to healthy individuals, while levels of AngII and Ang-(1-7) are lower. Even when excluding COVID-19 patients under RAS blockade treatment, ACE2 expression and enzymatic activity remain higher in COVID-19 patients, suggesting the involvement of RAS in COVID-19.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Geriatrics & Gerontology
Robert MacLachlan, Patrick Gavin Kehoe, J. Scott Miners
Summary: The activity of angiotensin-converting enzyme-1 (ACE-1) is induced in the early stages of Alzheimer's disease (AD), independently from the normal physiological changes in ACE-1 protein during aging.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Atila Duque Rossi, Joao Locke Ferreira de Araujo, Tailah Bernardo de Almeida, Marcelo Ribeiro-Alves, Camila de Almeida Velozo, Jessica Maciel de Almeida, Isabela de Carvalho Leitao, Samila Natiane Ferreira, Jessica da Silva Oliveira, Hugo Jose Alves, Helena Toledo Scheid, Debora Souza Faffe, Rafael Mello Galliez, Renata Eliane de Avila, Gustavo Gomes Resende, Mauro Martins Teixeira, Orlando da Costa Ferreira Junior, Terezinha Marta P. P. Castineiras, Renan Pedra Souza, Amilcar Tanuri, Renato Santana de Aguiar, Shana Priscila Coutinho Barroso, Cynthia Chester Cardoso
Summary: ACE2 and TMPRSS2 levels are positively correlated with age, which is strongly associated with respiratory distress. Increased ACE2 levels have a protective effect against this outcome, while the TMPRSS2/ACE2 ratio is associated with risk. In modeling COVID-19 severity, the TMPRSS2/ACE2 ratio outperforms ACE2.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Zijiao Qiao, Jiaqi Wang, Zeqi He, Lina Pan, Konglong Feng, Xiaoyu Peng, Qianru Lin, Yu Gao, Mingyue Song, Sufang Cao, Yunjiao Chen, Yong Cao, Guo Liu
Summary: In this study, an ACE-inhibitory peptide was separated and identified from goat milk and found to have potential anti-hypertensive effects. The peptide was able to alleviate the adverse effects of Ang II on vascular smooth muscle cells by inhibiting their proliferation and migration. Transcriptomic analysis revealed the peptide's ability to regulate pathways related to vascular remodeling.
FRONTIERS IN NUTRITION
(2022)
Article
Medicine, Research & Experimental
Jing-Yuan Xu, Wei Chang, Qin Sun, Fei Peng, Yi Yang
Summary: The study found that plasma midkine levels were significantly elevated in sepsis patients and closely related to the ACE system. In a mouse model, increased midkine levels were associated with more severe lung injury. Regional interference of midkine expression in lung tissue was shown to alleviate acute lung injury.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biology
Hristo L. Svilenov, Florent Delhommel, Till Siebenmorgen, Florian Ruehrnoessl, Grzegorz M. Popowicz, Alwin Reiter, Michael Sattler, Carsten Brockmeyer, Johannes Buchner
Summary: The solution structure, stability, and dynamics of a broadly-acting antiviral ACE2-IgG-Fc fusion protein are determined. Small chemical compounds binding to ACE2 can be used to drastically increase the thermal stability of the ACE2 domain. Our findings reveal a general concept for stabilizing the labile receptor segments of therapeutic antiviral fusion proteins by chemical compounds.
COMMUNICATIONS BIOLOGY
(2023)
Review
Food Science & Technology
Avtar Singh, Ajay Mittal, Soottawat Benjakul
Summary: This article provides insights into the applications of chitosan and its derivatives in the food industry, specifically for enhancing shelf-life and antimicrobial activity.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Food Science & Technology
Ajay Mittal, Avtar Singh, Hui Hong, Soottawat Benjakul
Summary: Chitooligosaccharides (COS) prepared from shrimp shell chitosan using different concentrations of H2O2 or AsA/H2O2 redox pair were characterized. COS-RP produced by AsA/H2O2 redox pair showed higher antioxidant and antimicrobial activities compared to COS-HP, with lower degree of polymerisation and average molecular weight.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Ting Zhou, Yi-Xuan Ding, Soottawat Benjakul, Shan -Shan Shui, Bin Zhang
Summary: This study investigated the changes in trypsin, calpain, and cathepsin activity and their effects on muscle proteins in intact and beheaded shrimp during frozen storage. The result showed that the beheading treatment effectively prolonged the shelf-life of stored shrimp products by inhibiting the degradation of muscle proteins. The beheaded shrimp samples exhibited more stable characteristics and significantly lower activities of trypsin, calpain, and cathepsin compared to intact shrimp samples throughout the storage period.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Umesh Patil, Jirakrit Saetang, Bin Zhang, Soottawat Benjakul
Summary: Freeze-dried tuna pepsin powder (TPP) was prepared using maltodextrin and trehalose, while trypsin-loaded beads (TLB) with glycerol were obtained via chitosan/alginate ionotropic gelation. TPP and TLB showed storage stability and proteolytic activity in simulated in vitro gastrointestinal (GI) tract conditions, effectively hydrolyzing various proteins. Peptides generated after GI digestion could pass through Caco-2 cell monolayers and exhibited different molecular size distribution and antioxidant activities. This suggests the potential use of TPP and TLB for enzyme supplementation and the improvement of human health through food-derived bioactive peptides.
Article
Food Science & Technology
Jirayu Buatong, Ajay Mittal, Pimonsri Mittraparp-arthorn, Suriya Palamae, Jirakrit Saetang, Soottawat Benjakul
Summary: The antibacterial effect of chitooligosaccharide conjugated with five different polyphenols against Listeria monocytogenes was investigated. Among all the conjugates tested, COS-EGCG showed the highest inhibition toward Listeria monocytogenes, with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 1024 μg/mL, respectively. The COS-EGCG conjugate also had a bactericidal effect on the environmental and clinical strains of L. monocytogenes.
Article
Chemistry, Applied
Mohamed Tagrida, Krisana Nilsuwan, Saqib Gulzar, Thummanoon Prodpran, Soottawat Benjakul
Summary: The mechanical, barrier, and thermal properties of biodegradable films composed of gelatin/chitosan blends with and without betel leaf ethanolic extract (BLEE) were investigated. The addition of BLEE improved the elasticity and heat-seal ability of the films, while the seal ability decreased with increasing chitosan proportion. Films containing 2% BLEE exhibited enhanced ultraviolet and visible light barrier abilities and reduced water vapor permeability. The incorporation of BLEE also led to decreased swelling and water solubility of the films, increased antioxidant and antibacterial activities, and improved thermal stability. Therefore, gelatin/chitosan films containing BLEE could serve as active packaging materials with satisfactory properties.
FOOD HYDROCOLLOIDS
(2023)
Article
Food Science & Technology
Natchaphol Buamard, Avtar Singh, Bin Zhang, Hui Hong, Prabjeet Singh, Soottawat Benjakul
Summary: The quality of surimi gel can be improved by using protein cross-linkers, especially those from plant extracts like Duea ching fruit. The fruit extract, rich in phenolic compounds and calcium, can activate transglutaminase and enhance the gel's properties. Different media for the extraction of Duea ching were studied and its application in sardine surimi gel was investigated.
Review
Immunology
Bakthavatchalam Loganathan Ayilya, Akshad Balde, Murugadoss Ramya, Soottawat Benjakul, Se-Kwon Kim, Rasool Abdul Nazeer
Summary: Acute lung injury can lead to chronic conditions such as idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD), asthma, and alveolar sarcoma. Studies are being conducted worldwide to understand the pathophysiology of these diseases and develop new treatments. Animal models, particularly mice, are commonly used to simulate disease conditions and study the effects of chemical inducers like Bleomycin (BLM), which targets receptors and inflammatory pathways. However, there is variation in BLM induction studies, highlighting the need for further research to understand its molecular mechanisms of action. This review discusses different chemical inducers, the mechanism of BLM-induced lung injury, and the advantages and disadvantages of using BLM in animal models.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dinakar Swastha, Nambolan Varsha, Suresh Aravind, Kavassery Balasubramanian Samyuktha, Muruganandam Mohaneswari Yokesh, Akshad Balde, Bakthavatchalam Loganathan Ayilya, Soottawat Benjakul, Se -Kwon Kim, Rasool Abdul Nazeer
Summary: Inflammatory bowel disease (IBD) is a common inflammatory disorder affecting the gastrointestinal tract. Alginate (AG), as a marine-derived polysaccharide, has been extensively studied for its properties in drug delivery systems for IBD treatment. AG-based carrier systems, including nanotechnology-based systems and hydrogels, have shown potential in targeted drug delivery and tissue regeneration. This review focuses on the pathogenesis of IBD, currently studied medications, AG-based drug delivery systems, and their properties for alleviating IBD.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Avtar Singh, Saumya Mehta, Umesh Patil, Pornpot Nuthong, Soottawat Benjakul
Summary: The effects of adding hyperoside (HyS) at various levels (0%-4%; w/w) on the textural, rheological, structural, and sensory properties of threadfin bream surimi were investigated. The addition of 1% and 2% HyS resulted in surimi gel with the highest breaking force and deformation. HyS also improved the water-holding capacity and increased hardness, springiness, gumminess, and chewiness of the gel. The addition of HyS enhanced the connections between surimi proteins and improved the gel network.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Fisheries
Wattana Temdee, Avtar Singh, Hui Hong, Bin Zhang, Ahmet Faruk Yesilsu, Soottawat Benjakul
Summary: The effects of epigallocatechin gallate (EGCG) at 200 or 400 ppm on the quality changes of cooked Harpiosquillid mantis shrimp (Harpiosquilla raphidea) (CHMS) meat subjected to varying freeze-thaw cycles (F-T-C; 0, 1, 3, and 5) were studied. With increasing F-T-C, the CHMS meat deteriorated to a higher extent, indicated by increased exudate loss, tougher texture, and higher microbial counts. The addition of EGCG could dose-dependently retard these changes, as well as prevent microbial growth and lipid oxidation. The alterations in disulfide bond content and surface hydrophobicity of CHMS added with EGCG were more pronounced with higher EGCG concentration.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Acoustics
Bharathipriya Rajasekaran, Avtar Singh, Arunachalasivamani Ponnusamy, Umesh Patil, Bin Zhang, Hui Hong, Soottawat Benjakul
Summary: This study investigated the effects of ultrasonication on the physicochemical and emulsifying properties of fish myofibrillar protein (FMP) at different amplitudes and durations. The results showed that ultrasonication at 40% amplitude for 15 minutes significantly improved the solubility, surface hydrophobicity, and emulsifying properties of FMP. Protein pattern analysis revealed changes in the myosin heavy chain. Ultrasound treatment also increased the adsorption of FMP on oil droplets and altered the chemical bonds. Furthermore, emulsion stabilized by ultrasound-treated FMP exhibited higher stability, smaller droplet sizes, and altered rheological behavior.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Fisheries
Yingru Wu, Qi Du, Yueqin Liao, Shanshan Shui, Jie Pang, Soottawat Benjakul, Bin Zhang
Summary: This study aimed to investigate the effects of different soaking methods on the quality changes of chilled ready-to-eat peeled shrimp. The results showed that the group soaked in a solution containing 4% NaCl, 0.34% citric acid, 4.23% trehalose, and 0.04% VC had a significantly longer shelf-life and better sensory scores and color compared to the control group. The volatile organic compounds (VOCs) in the shrimp muscle also had smaller changes in this group during chilled storage.
Article
Biotechnology & Applied Microbiology
Jaksuma Pongsetkul, Soottawat Benjakul, Pakpoom Boonchuen
Summary: This study explored the potential use of Bacillus subtilis K-C3 as a starter to enhance the quality of shrimp paste, with a focus on its nutritional composition. The study also examined the changes in quality and characteristics of shrimp paste during 18 months of storage at low and room temperatures, with and without bacterial inoculation. The results revealed that the B. subtilis strain increased the levels of essential amino acids, polyunsaturated fatty acids, and antioxidant properties in the experimental shrimp paste compared to traditional shrimp paste. The study also found that storage at room temperature accelerated the changes in quality and characteristics of the shrimp paste, while storage at low temperature led to yeast and mold growth, indicating food spoilage. Overall, inoculation with B. subtilis K-C3 and storage at room temperature improved the nutritional profile and safety of shrimp paste, extending its shelf-life to at least 18 months.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
Mohamed Tagrida, Suriya Palamae, Jirakrit Saetang, Lukai Ma, Hui Hong, Soottawat Benjakul
Summary: The antibacterial activities of quercetin and hyperoside were evaluated against two major spoilage bacteria in fish. Hyperoside showed stronger antibacterial effects than quercetin with lower concentrations. Both compounds affected cell membrane morphology and induced the release of cell contents. They also inhibited bacterial motility and biofilm formation. Additionally, they could bind to or degrade bacterial genomic DNA, leading to bacterial death. Therefore, quercetin and hyperoside have the potential to be used as antimicrobial agents to prevent fish or perishable product spoilage.