Article
Materials Science, Paper & Wood
Dominic W. S. Wong, Victor J. Chan, Hans Liao
Summary: A feruloyl esterase (FAE) gene was isolated from rumen microbial metagenome, expressed and purified successfully. FAE efficiently catalyzed the release of ferulic acid (FA) from corn fiber under optimal conditions. Addition of GH10 endoxylanase (XYN) enhanced the yield of FAE in releasing FA.
Article
Microbiology
Yao Zhang, Zhilin Jiang, Yunran Li, Zhiping Feng, Xian Zhang, Ruiping Zhou, Chao Liu, Lijuan Yang
Summary: This study found that the synergistic effect of multiple bacteria can increase the production of ferulic acid, and optimal fermentation conditions were determined through orthogonal design, showing that a combination of Klebsiella oxytoca Z28, Klebsiella pneumoniae JZE, and Bacillus velezensis G1 can achieve the best results.
Article
Biotechnology & Applied Microbiology
Takehiko Todokoro, Hiroaki Negoro, Atsushi Kotaka, Yoji Hata, Hiroki Ishida
Summary: This study found that disrupting the faeA gene in Aspergillus oryzae is an effective strategy to reduce the common off-flavor, 4-VG, in sake. A faeA-deficient strain was created using genome co-editing, and fermentation was conducted using a Saccharomyces cerevisiae strain capable of converting ferulic acid to 4-VG. The results showed a significant decrease in 4-VG content and sensory evaluation revealed minimal detection of 4-VG.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Agricultural Engineering
Shujun Liu, Lubna Soomro, Xue Wei, Xufeng Yuan, Tianyi Gu, Zhen Li, Yulu Wang, Yuming Bao, Fengzhong Wang, Boting Wen, Fengjiao Xin
Summary: By enhancing the structure of feruloyl esterase and selecting more efficient mutants, this study successfully increased the yield of ferulic acid, providing a new direction for industrial production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Kamyar Mogodiniyai Kasmaei, Dayanand C. Kalyani, Tom Reichenbach, Amparo Jimenez-Quero, Francisco Vilaplana, Christina Divne
Summary: This study presents the crystal structure and biochemical characterization of a feruloyl esterase from Lentilactobacillus buchneri, a lactic acid bacteria strain. The feruloyl esterase exhibits narrow specificity, accommodating only single FA chains attached to arabinoxylan. This research is important for the potential applications of lactic acid bacteria-derived feruloyl esterases in the food and pharmaceutical industries.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sangeun Jeon, Jisub Hwang, Hackwon Do, Ly Thi Huong Luu Le, Chang Woo Lee, Wanki Yoo, Min Ju Lee, Seung Chul Shin, Kyeong Kyu Kim, Han-Woo Kim, Jun Hyuck Lee
Summary: In this study, a feruloyl esterase (LaFae) from Lactobacillus acidophilus was obtained and characterized. LaFae could effectively produce ferulic acid from wheat bran, rice bran, and corn stalks. Xylanase supplementation was found to enhance LaFae activity, increasing ferulic acid production. The crystal structures of LaFae revealed the active site configuration and the role of a gatekeeper residue in substrate binding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Alice L. Turner, Louise V. Michaelson, Peter R. Shewry, Alison Lovegrove, Jeremy P. E. Spencer
Summary: In this study, intake of high fiber bread containing higher levels of free ferulic acid was found to enhance human endothelium-dependent vascular function by increasing vascular dilation to improve cardiovascular health. Enzymatically processed high fiber bread releasing FA also increased the bioavailability of FA.
CLINICAL NUTRITION
(2021)
Article
Biotechnology & Applied Microbiology
Fulong Man, Huaran Xing, Haoran Wang, Junfeng Wang, Rong Lu
Summary: In this study, a new platform named Esterase-responsive Active Loading (EAL) was developed for efficient and stable drug encapsulation of small extracellular vesicles (sEVs). The EAL utilized engineered sEVs to establish a continuous transmembrane ion gradient for active loading of the drug ferulic acid. The results showed that the EAL significantly improved drug loading and encapsulation efficiency compared to passive loading. Furthermore, extracellular and intracellular assessments confirmed the slow release and low toxicity of the EAL-prepared sEVs-loaded ferulic acid preparation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Jiaxin Lan, Shujie Ji, Chuanjia Yang, Guolin Cai, Jian Lu, Xiaomin Li
Summary: This study selected enzymes from the metagenomes of a cow rumen and a camel rumen for recombinant expression in Escherichia coli, aiming to release ferulic acid from agricultural residues. The strain expressing both enzymes showed the best performance in degrading biomass and releasing ferulic acid, achieving the highest obtained value. This result has potential implications for the re-utilization of agricultural residues and enhancing their value.
Article
Microbiology
Yao Zhang, Zhiping Feng, Hongzhu Xiang, Xian Zhang, Lijuan Yang
Summary: A strain of Klebsiella oxytoca Z28 with ferulic esterase activity was isolated from Daqu, and the FAE gene was expressed in Escherichia coli BL21 (DE3). The enzyme showed good stability and activity at favorable temperature and pH. KoFAE from Klebsiella oxytoca Z28 has the potential to degrade agricultural waste and produce high value-added ferulic acid products when expressed heterologously in E. coli.
Article
Chemistry, Multidisciplinary
Wenbin Zhang, Jieyu Wang, Yuechen Liu, Juncai Leng
Summary: This study successfully enhanced the efficient expression of ferulic acid esterase and demonstrated its potential biodegradation capability in sunflower seed processing.
APPLIED SCIENCES-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Abha Sharma, Jyoti Singh, Pushpendra Sharma, Govind Singh Tomar, Surender Singh, Minakshi Grover, Lata Nain
Summary: This study focuses on the production of biovanillin through microbial biotransformation of agrowastes using Streptomyces sp. ssr-198. The strain was able to produce vanillin and vanillic acid, with varying enzyme activities and production levels under different culture conditions. Additionally, a synergistic effect was observed when using ferulic acid esterase from Enterococcus lactis SR1 in combination with Streptomyces sp. ssr-198 for biovanillin production using agro residues.
Article
Biochemistry & Molecular Biology
Shang Lin, Cameron J. Hunt, Jesper Holck, Jesper Brask, Kristian B. R. M. Krogh, Anne S. Meyer, Casper Wilkens, Jane W. Agger
Summary: The study found that esterases from various bacteria and fungi can catalyze the release of diferulic acids from complex arabinoxylans. By comparing the activity of the enzymes on synthetic substrates and diFA release, a detailed structure-function analysis is needed to explain the diFA releasing ability of the enzymes. The findings contribute to a better understanding of the role of esterases in the degradation of complex arabinoxylans and provide new opportunities for enzyme-assisted upgrading of complex bran arabinoxylans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Archontoula Giannakopoulou, Georgia Tsapara, Anastassios N. Troganis, Panagiota Koralli, Christos L. Chochos, Angeliki C. Polydera, Petros Katapodis, Nektaria-Marianthi Barkoula, Haralambos Stamatis
Summary: In this study, a series of polymers were enzymatically functionalized with ferulic acid, resulting in improved antioxidant properties and antimicrobial activity. The successful grafting of oxidized ferulic acid onto the polymers was confirmed, and the modified polymers showed potential application in various industries.
Article
Agriculture, Multidisciplinary
Shang Lin, Jesper Brask, Line Munk, Jesper Holck, Kristian B. R. M. Krogh, Anne S. Meyer, Jane Wittrup Agger, Casper Wilkens
Summary: A new enzyme activity has been discovered that can directly release diferulic acids from corn bran, providing new insights and opportunities for solving the problem of recalcitrant arabinoxylans.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)