期刊
JOURNAL OF FOOD QUALITY
卷 35, 期 1, 页码 76-82出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1745-4557.2011.00423.x
关键词
-
资金
- Shanghai Science and Technology Committee [10DZ0502600]
An indirect competitive enzyme-linked immunosorbent assay with chemiluminescent (CL-ELISA) detection for okadaic acid (OA) in mussel muscle was developed. A hybridoma cell line secreting monoclonal antibody against OA was established after immunization of BALB/c mice with artificially synthesized OA-bovine serum albumin as antigen. Luminol solution was used as the substrate for horseradish peroxidase. The detection limit was 0.175 ng/g. The range of average fortified recovery was 91.8-102.6% when OA was spiked in mussel muscle at levels of 50, 160 and 800 mu g/kg. The standard curve for OA showed good linearity over the concentration range of 0.08125-20 ng/mL. The cross-reactivity with dinophysistoxin1 and saxitoxin was 51.82 and 0%, respectively. In a residue study, the results obtained by CL-ELISA correlated well within those obtained using the commercial ABRAXIS kit (ABRAXIS, Warminster, PA). The developed method is therefore suitable for detecting the residues of OA in shellfish.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据