期刊
JOURNAL OF FOOD PROTECTION
卷 72, 期 7, 页码 1476-1480出版社
INT ASSOC FOOD PROTECTION
DOI: 10.4315/0362-028X-72.7.1476
关键词
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资金
- Ministry of Agriculture, Forestry and Fisheries of Japan [DI-208, FP-6104]
- Food Safety Commission of Japan [0605]
- Ministry of Education, Science, Sports, and Culture of Japan [B20380121]
Listeria monocytogenes is of great concern as a foodborne pathogen. Many ready-to-eat foods are widely contaminated with this organism and have caused listeriosis outbreaks and sporadic cases in many countries. In Japan, there is a high incidence of L. monocytogenes contamination, specifically in raw ready-to-eat seafood. Identical L. monocytogenes subtypes have been isolated repeatedly from samples of food manufactured at a given store or processing plant, and researchers suspected that certain L. monocytogenes isolates have formed biofilms at these sites. A microtiter plate biofilm formation assay was conducted, and all raw ready-to-eat seafood isolates tested were able to form biofilms to various degrees. Biofilm formation by L. monocytogenes isolates of lineage I was significantly greater (P = 0.000) than that by isolates of lineage II. However, isolates of clonal lineages formed different levels of biofilms, indicating that the ability to form a biofilm is affected positively or negatively by environmental factors.
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