SIMULTANEOUS DETERMINATION OF SEVEN FLAVONOIDS IN DAN BAMBOO PHYLLOSTACHYS GLAUCA McCLURE LEAF EXTRACT AND IN COMMERCIAL PRODUCTS BY HPLC-DAD

Flavonoids are a group of polyphenolic compounds with health-related properties. The leaves of Phyllostachys glaucaMcClure are rich in flavonoids and its extracts and extract products are the functional ingredients in several commercial products, such as food additives. A new high-performance liquid chromatography (HPLC) technique was developed and used to analyze seven flavonoids in commercial extract products and P.glaucaMcClure leaf extract. The leaf extract was purified using column chromatography with macroporous absorption resin (AB-8) before analysis. After a 2-h hydrolysis, the seven flavonoids were simultaneously quantified using HPLC with an ultraviolet photodiode-array detector at 350nm. Identification of the seven flavonoids (isoorientin, orientin, vitexin, isovitexin, luteolin-6-C-arabinoside, luteolin and tricin) was confirmed by a liquid chromatography mass spectrometer system. Validation was achieved, proving that this method can be used to determine trace amounts of these seven flavonoids in P.glaucaMcClure leaf extracts and in commercial extract products.