Article
Microbiology
Barbara F. Nowak, Mai Dang, Claire Webber, Lukas Neumann, Andrew Bridle, Roberto Bermudez, Daryl Evans
Summary: The study found that Southern Bluefin Tuna showed the same pattern in changes in the surface area of MMCs, but blood fluke infection varied significantly between different cohorts and individual fish within the same cohort. Cardicola forsteri had a higher prevalence than Cardicola orientalis. This research contributes to our understanding of blood fluke infections in Southern Bluefin Tuna and their interactions with MMCs.
Article
Biochemistry & Molecular Biology
Daniel H. Scharf, Pranatchareeya Chankhamjon, Kirstin Scherlach, Jan Dworschak, Thorsten Heinekamp, Martin Roth, Axel A. Brakhage, Christian Hertweck
Summary: Gliotoxin and related ETPs from fungi feature highly functionalized hydroindole scaffolds with a wide range of medically and ecologically relevant activities. The cytochrome P450 monooxygenase, GliF, from the human-pathogenic fungus Aspergillus fumigatus plays a key role in the formation of complex heterocycles specific to ETPs, catalyzing an unprecedented heterocyclization reaction that cannot be replicated with current synthetic methods. In silico analyses suggest that this rare biotransformation occurs in related ETP biosynthetic pathways.
Article
Environmental Sciences
Rebecca Whitlock, Robert J. Schallert, Michael J. W. Stokesbury, Michael Castleton, Barbara A. Block
Summary: Understanding the productivity of marine fish and wildlife populations is crucial for their sustainable management. This study evaluates the potential of acoustic telemetry as a long-term monitoring tool to estimate rates of natural mortality and finds that the use of satellite tags can improve the accuracy and precision of the monitoring process.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Physiology
Takashi Kitagawa, Takaaki K. Abe, Keitaro Kubo, Ko Fujioka, Hiromu Fukuda, Yosuke Tanaka
Summary: This study aimed to clarify the development of the ability of Pacific bluefin tuna (PBT) to maintain a high body temperature. The research found that PBT can maintain higher body temperatures as they grow and their ability to maintain a high body temperature is independent of the surrounding ambient temperature when the fish reach a certain size. The study also revealed the changes in whole-body heat-transfer coefficient and heat-production rate during growth.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
John A. Mohan, Heidi Dewar, Owyn E. Snodgrass, Nathan R. Miller, Yosuke Tanaka, Seiji Ohshimo, Jay R. Rooker, Malcom Francis, R. J. David Wells
Summary: Understanding the movement behaviors and population connectivity of highly migratory fishes like bluefin tuna is crucial for their conservation and management. This study analyzed otolith biominerals of large Pacific bluefin tuna to investigate patterns of trace element distribution across ontogeny. The results showed enrichment and subsequent decrease in element:Ca ratios, indicating potential influences of thermal and metabolic physiologies, common diets, or ambient water chemistry during early life. Variability in other otolith elements differed by capture region, suggesting the influence of ocean-specific environmental factors or growth-related physiologies on otolith mineralization.
Article
Cell Biology
Dongqin Wei, Xin Tian, Longbo Zhu, Han Wang, Chao Sun
Summary: This study revealed the important role of ubiquitin-specific proteinase 14 (USP14) in the progression of nonalcoholic fatty liver disease (NAFLD). The overexpression of USP14 exacerbated the progression of NAFLD, while its knockdown had a mitigating effect. Furthermore, USP14 was found to promote lipid peroxidation and inflammation by regulating the protein level of cytochrome p4502E1 (CYP2E1). The study also demonstrated that USP14 facilitated the accumulation of CYP2E1 protein by inhibiting the degradation of heat shock protein 90 alpha family class A member 1 (HSP90AA1).
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Lachlan Coff, Andrew J. Guy, Bronwyn E. Campbell, Barbara F. Nowak, Paul A. Ramsland, Nathan J. Bott
Summary: Genomic sequencing and assembly of the pathogen Cardicola forsteri were performed, revealing a high-quality contig-level assembly. The genome consists of a large portion of repetitive elements and coding regions, with the identification of numerous hypothetical polypeptides.
Article
Ecology
Oyvind Fiksen, Patricia Reglero
Summary: The survival and growth of bluefin tuna during early life stages are influenced by prey availability and temperature. High temperatures are not ideal for feeding larvae, but heatwaves can increase larval survival potential while shortening the viable season for them. Food resources play a key role in modulating how temperature change impacts optimal phenology for the tuna larvae.
Article
Biochemistry & Molecular Biology
Yasuhiro Yonezuka, Hiroki Kuwada, Hiromasa Imaishi
Summary: This study demonstrated the effectiveness of the P450 inhibition assay in a mouse model of DILI, suggesting its potential for diagnosing liver diseases such as acute DILI.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Environmental Sciences
Thomas R. Carruthers, Quang C. Huynh, Matthew V. Lauretta, Ann L. Preece, Douglas S. Butterworth, Adrian R. Hordyk, Nathan G. Taylor
Summary: This study implemented an individual tagging model to evaluate the benefits of a harvest strategy using conventional gene tagging for Atlantic bluefin tuna. Different scenarios for release numbers and genotyping rates were tested to assess the yield and resource conservation performance. The results showed that the gene tagging harvest strategies offered similar performance to idealized management in terms of yield and resource conservation.
FRONTIERS IN MARINE SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Joanna Jastrzebska, Wladyslawa Anna Daniel
Summary: Cytochrome P450 is involved in the metabolism of drugs, substances of abuse, and endogenous substrates. Cocaine affects the dopaminergic system and may also alter the regulation of cytochrome P450 during the addiction process. The effects of cocaine on liver cytochrome P450 enzymes depend on various factors and can lead to hepatotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Soudeh Bahramian Nasab, Ahmad Homaei, Roberto Fernandez-Lafuente, Jon Del Arco, Jesus Fernandez-Lucas
Summary: The use of marine enzymes as catalysts for biotechnological applications is a hot topic. In this study, a liver NADPH-dependent cytochrome P450 reductase (CPR) from the marine fish Liza klunzingeri (LkCPR) was isolated, purified and biochemically characterized. LkCPR showed remarkable catalytic features, such as a wide range of pH and temperature stability, high catalytic activity, and highest thermostability among reported CPRs. These findings suggest that LkCPR has great potential as a biocatalyst.
Article
Multidisciplinary Sciences
Laurentijn Tilleman, Kaat Rubben, Wim Van Criekinge, Dieter Deforce, Filip Van Nieuwerburgh
Summary: This study tested the effectiveness of Targeted Locus Amplification (TLA) combined with Illumina or Nanopore sequencing in accurate genotyping and phasing of pharmacogenes. The results showed limited nucleotide mapping on target and incomplete coverage of genes with both sequencing methods. However, the optimized primer pair for the CYP2D6 gene in the Nanopore dataset resulted in correct haplotypes, indicating the potential reliability of this method with patient-specific primer design and optimization.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chun-Mao Tseng, Shin-Jing Ang, Yi-Sheng Chen, Jen-Chieh Shiao, Carl H. Lamborg, Xiaoshuai He, John R. Reinfelder
Summary: Research shows that the mercury accumulation rate in bluefin tuna is highest in the Mediterranean Sea, decreases in the North Pacific, Indian Ocean, and North Atlantic Ocean, correlating with the concentrations of MeHg in regional seawater and zooplankton. These differences are related to the levels of mercury in each ocean subbasin, geogenic enrichment, and anthropogenic contamination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Fisheries
Aldo Corriero, Matthew J. Wylie, Mitsuo Nyuji, Rosa Zupa, Constantinos C. Mylonas
Summary: This article reviews the reproductive biology of Carangidae species for aquaculture industry, comparing wild and farmed fish reproductive traits and summarizing hormonal maturation and spawning induction protocols to improve reproductive dysfunctions and obtain fertile gametes.
REVIEWS IN AQUACULTURE
(2021)
Article
Agriculture, Dairy & Animal Science
Giambattista Bello, Nicoletta Santamaria, Aldo Corriero
Summary: Most fish undergo distinct growth phases during ontogenesis, with a crucial passage from juvenile to adult phase occurring at the onset of sexual maturity, leading to changes in body proportion and/or growth rate. These changes can be detected as change-points in biometric relationships.
Review
Fisheries
Aldo Corriero, Rosa Zupa, Constantinos C. Mylonas, Letizia Passantino
Summary: Atresia of ovarian follicles, a normal component of fish oogenesis, can reduce fish fecundity and lead to reproductive failure when occurring above physiological rates, impacting fisheries and aquaculture.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Chrysovalentinos Pousis, Mariasevera Di Comite, Rosa Zupa, Letizia Passantino, Edmond Hala, Aldo Corriero
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Osvalda De Giglio, Francesco Triggiano, Francesca Apollonio, Chrysovalentinos Pousis, Carla Calia, Giusy Diella, Francesco Bagordo, Sapia Murgolo, Tiziana Grassi, Cristina De Ceglie, Silvia Brigida, Giuseppina La Rosa, Pamela Mancini, Giusy Bonanno Ferraro, Antonella De Donno, Giuseppe Mascolo, Maria Clementina Caputo, Maria Teresa Montagna
Summary: This study evaluated the presence of chemical and microbial contaminants in treated wastewater and their impact on groundwater. The results showed that the porous vadose zone is more effective than the fractured karst vadose zone in reducing contaminant loads.
Article
Agriculture, Dairy & Animal Science
V. Tufarelli, L. Passantino, R. Zupa, P. Crupi, V. Laudadio
Summary: This study assessed the effect of dietary dried olive pulp (DOP) on growth performance, meat traits, oxidation, and intestinal mucosa features in broiler chickens. The results showed that including 10% of DOP in the diet can improve meat quality, intestinal morphology, and productive traits of broiler chickens. This suggests that olive by-product can be a cost-effective replacement for conventional feeds in poultry production.
Article
Agriculture, Dairy & Animal Science
Rosa Zupa, Edmond Hala, Gianluca Ventriglia, Chrysovalentinos Pousis, Letizia Passantino, Angelo Quaranta, Aldo Corriero, Caterina De Virgilio
Summary: The study assessed the reproductive maturation of meagre reared in sea cages under routine farming conditions. It was observed that meagre males and females, produced in hatcheries and reared in sea cages, are able to spontaneously produce and release gametes. This suggests that meagre reproduction under commercial conditions could expand meagre aquaculture production, which currently relies on limited fingerling production by a select few hatcheries.
Article
Veterinary Sciences
Luisa Valentini, Rosa Zupa, Chrysovalentinos Pousis, Rezart Cuko, Aldo Corriero
Summary: This study found that cat spermatogenesis is seasonally regulated, with increased spermatogonial proliferation during the reproductive period and increased germ cell apoptosis during the non-reproductive period.
VETERINARY SCIENCES
(2022)
