期刊
JOURNAL OF FISH DISEASES
卷 35, 期 4, 页码 265-273出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2761.2011.01342.x
关键词
GFP-labelled pathogen; gnotobiotic model system; larviculture; microscopy; ultrastructure
资金
- Ghent University (Belgium) [nr 05B01906, 01J01206]
- Foundation for Scientific Research-Flanders (FWO) [G.0491.08]
- European Community [227197]
The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V.similar to anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V.similar to anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.
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