Effects of antinutritional factors on plasma lipoprotein levels in Japanese flounder Paralichthys olivaceus

This study examined the effects of four types of antinutritional factor (phytic acid, stachyose, soy saponins and soy isoflavones) on lipoprotein levels in plasma of Japanese flounder Paralichthys olivaceus. A basal diet was prepared with fish meal as primary protein source, the other diets were supplemented with 0.2, 0.4 or 0.8% phytic acid, 0.4, 0.8 or 1.5% stachyose, 0.1, 0.35 or 0.7% soy saponins and 0.10, 0.35 or 0.70% soy isoflavones, by dry mass, in place of white flour in the basal diet. Total cholesterol (TC) and triglyceride (TG) levels in plasma of P. olivaceus were not affected by phytic acid or stachyose. In general, addition of 0.2-0.8% phytic acid or 0.4-1.5% stachyose decreased plasma high-density lipoprotein cholesterol (HDL-C) levels, increased plasma low-density lipoprotein cholesterol (LDL-C) levels, thereby increasing the LDL-C:HDL-C ratio. By contrast, supplementation with 0.35-0.7% soy saponins generally depressed plasma TC levels and the LDL-C:HDL-C ratio. Supplementation with 0.35-0.7% soy isoflavones, however, increased plasma TC and TG levels. These results indicate that soy saponins may be partly responsible for the cholesterol-lowering effects of soybean meal. (C) 2011 The Authors Journal of Fish Biology (C) 2011 The Fisheries Society of the British Isles