期刊
JOURNAL OF FISH BIOLOGY
卷 77, 期 2, 页码 341-360出版社
WILEY
DOI: 10.1111/j.1095-8649.2010.02676.x
关键词
differential gene expression; Epinephelus coioides; microarray; Singapore grouper iridovirus; suppression subtractive hybridization
资金
- Chinese Academy of Sciences [KZCX2-YW-BR-08]
- National Basic Research Program of China [2006CB101802]
- Natural Science Foundation of China [30725027, 30930070]
- Natural Science Foundation of Guangdong, China [06104920]
- Science and Technology Program of Guangdong [2006B50104003]
Suppression subtractive hybridization (SSH) was used to generate a subtracted cDNA library enriched with gene transcripts differentially expressed in the spleen of orange-spotted grouper Epinephelus coioides after 5 days of infection with Singapore grouper iridovirus (SGIV). In the forward and reverse-subtracted libraries, 260 and 153 non-redundant expressed sequence tags (EST), respectively, were identified. These annotated genes responding to SGIV infection were grouped into eight gene categories related to immunity, cell structure, transcription-translation, cell signalling, metabolism, mitochondrial proteins, ribosomal proteins and unknown or hypothetical proteins. A DNA microarray containing all the differentially expressed genes was constructed, and the gene expression patterns in different tissues were investigated in virus-infected E. coioides. Of these genes, four associated with the infection processes were identified and further investigated by quantitative real-time PCR. These results provide new insights into the molecular basis of host-pathogen interactions in E. coioides, and will help the development of control strategies against SGIV infection.
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