Article
Chemistry, Medicinal
Alireza Afshar, Arezoo Khoradmehr, Fariborz Nowzari, Neda Baghban, Masoud Zare, Maryam Najafi, Seyedeh Zahra Keshavarzi, Fatemeh Zendehboudi, Gholamhossein Mohebbi, Alireza Barmak, Fatemeh Mohajer, Nahid Basouli, Mohammadreza Keshtkar, Aida Iraji, Fatemeh Sari Aslani, Cambyz Irajie, Iraj Nabipour, Mehdi Mahmudpour, Nader Tanideh, Amin Tamadon
Summary: The study aimed to evaluate the wound healing properties of brittle star extracts in vitro and in vivo. Hydroalcoholic extracts of brittle star undergoing arm regeneration were evaluated for their wound healing effect. GC-MS analysis, MTT cell viability and migration assays, Western blot, and computational analysis tests were performed. The study showed positive proliferative effects of the extracts, with certain compounds exhibiting strong affinity with proteins. In vivo analysis demonstrated that the extracts from 7 and 14 days of arm regeneration promoted wound healing and had better wound healing outcomes compared to other groups.
Article
Biology
Anna Czarkwiani, Jack Taylor, Paola Oliveri
Summary: Brittle stars have the ability to fully regenerate their nerves, and understanding this process could provide insights for improving therapeutics for central nervous system injuries in humans.
Article
Cell Biology
Gurmannat Kalra, Daniell Lenz, Dunia Abdul-Aziz, Craig Hanna, Mahashweta Basu, Brian R. Herb, Carlo Colantuoni, Beatrice Milon, Madhurima Saxena, Amol C. Shetty, Ronna Hertzano, Ramesh A. Shivdasani, Seth A. Ament, Albert S. B. Edge
Summary: In this study, we examined the changes in chromatin accessibility and transcriptional programs during the differentiation of cochlear hair cells from postmitotic supporting cells using organoids. We identified Lgr5+ cells as the progenitors for hair cells and revealed the gene regulatory networks involved in their development. The analysis also predicted the involvement of additional regulatory factors in hair cell regeneration. This study provides important insights into the signals for hair cell regeneration from mammalian supporting cells.
Article
Respiratory System
Negah Ahmadvand, Farhad Khosravi, Arun Lingampally, Roxana Wasnick, Ana Ivonne Vazquez-Armendariz, Gianni Carraro, Monika Heiner, Stefano Rivetti, Yuqing Lv, Jochen Wilhelm, Andreas Gunther, Susanne Herold, Denise Al Alam, Chengshui Chen, Parviz Minoo, Jin-San Zhang, Saverio Bellusci
Summary: The study identified two distinct subpopulations of AT2 cells in mice, with Tom(Low) cells showing stem cell properties and being activated during lung regeneration, while Tom(High) cells represent mature AT2 cells. The presence of similar PD-L1(Pos) population was also observed in human lungs, indicating the existence of quiescent, immature AT2 progenitor cells in both species.
EUROPEAN RESPIRATORY JOURNAL
(2021)
Article
Cell Biology
Aaron J. Huebner, Rebecca A. Gorelov, Ruslan Deviatiiarov, Samuel Demharter, Tobias Kull, Ryan M. Walsh, Marty S. Taylor, Simon Steiger, John T. Mullen, Peter V. Kharchenko, Konrad Hochedlinger
Summary: Huebner et al. developed a 2D culture system to study gastric isthmus stem cells and found that Sox2 plays a role in specifying enterochromaffin cells in the stomach. They showed that cell types in the corpus and antrum regions share similar transcriptional states, including the poorly characterized isthmus stem cells. The 2D culture system can be converted into 3D organoids and was used to demonstrate the importance of Sox2 in generating enterochromaffin cells.
NATURE CELL BIOLOGY
(2023)
Article
Biology
Minyan Zheng, Olga Zueva, Veronica F. Hinman
Summary: The ability to restore lost body parts is a fascinating field of study, but there are still many unanswered questions regarding the origin and potential of regenerating cells, as well as the role of gene regulatory networks. In this study, new transgenic tools were developed to track cell populations in the regenerating larva of sea stars, and it was found that the larval serotonergic nervous system can regenerate following decapitation.
Review
Biochemistry & Molecular Biology
Natalia Smith-Cortinez, A. Katherine Tan, Robert J. Stokroos, Huib Versnel, Louise V. Straatman
Summary: Sensorineural hearing loss is caused by damage to sensory hair cells and/or spiral ganglion neurons. In non-mammalian species, hair cell regeneration after damage is observed, even in adulthood. This review discusses the pathways involved in the development of the cochlea and the impact that trauma has on the regenerative capacity of the endogenous progenitor cells. Furthermore, it discusses the effects of manipulating key signaling pathways targeting supporting cells with progenitor potential to promote hair cell regeneration and translates these findings to the human situation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Behavioral Sciences
Nasim Kiaie, Armita Mahdavi Gorabi, Reid Loveless, Yong Teng, Tannaz Jamialahmadi, Amirhossein Sahebkar
Summary: Cell therapeutic approaches for tissue regeneration have gained considerable attention in recent years. This review discusses the roles of glial progenitor cells and reactive glial cells in central nervous system (CNS) regeneration, as well as their involvement in CNS-related diseases.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2022)
Review
Cell Biology
Wenguang Liu, Meng Feng, Peng Xu
Summary: Growing evidence has shown the strong potential of cartilage-derived progenitor cells (CPCs) in proliferation, migration, and differentiation. While CPCs play a crucial role in cartilage development and repair in immature tissue, they are unable to fully repair cartilage defects during maturation and aging. Internal factors such as chronic inflammation and external factors like mechanical loading and nutritional imbalances can hamper the healing and regeneration of cartilage by CPCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuxi Sun, Tatiana Boyko, Owen Marecic, Danielle Struck, Randall K. Mann, Tom W. Andrew, Michael Lopez, Xinming Tong, Stuart B. Goodman, Fan Yang, Michael T. Longaker, Charles K. F. Chan, George P. Yang
Summary: Failure to form bone properly or integrate surgical implants can result in complications and additional interventions in orthopedic surgeries. The role of skeletal stem cells (SSCs) in bone formation is understood, but little is known about the regulation of Bone, Cartilage, Stromal, Progenitors (BCSPs). This study demonstrates that Del1 is a key regulator of BCSP expansion and its knockout leads to reduced bone formation. Del1 promotes BCSP proliferation and prevents apoptosis, making it a potential therapeutic target for improving bone formation and implant success.
Article
Polymer Science
Wencheng Shi, Boyu Wu, Xiaofeng Guo, Anchao Feng, San H. Thang
Summary: Fluorescent end-functional mikto-arm star copolymers were synthesized using a novel method and the linear correlation between fluorescence intensity and polymer concentration was verified. This method enables the quantitative analysis of the arm components of star polymers, providing a new approach for the characterization of star polymers.
Article
Biochemistry & Molecular Biology
Fan Lai, Jingjing Wang, Hong Tang, Pan Huang, Juan Liu, Gang He, Mei Zhou, Xu Tao, Kanglai Tang
Summary: The stem cell microenvironment plays a crucial role in stem cell maintenance or differentiation. The expression of IL-10, G-CSF, and VEGF showed significant differences between young and aged rats during patellar tendon repair. VEGF was found to have an inhibitive effect on the adipogenic differentiation of TPSCs. Furthermore, targeting SPP-1 improved the histological score and biomechanical property of aged tendon healing.
Review
Cell & Tissue Engineering
Yasmin K. Alshoubaki, Bhavana Nayer, Surojeet Das, Mikael M. Martino
Summary: The immune system has a significant impact on the regulation and regenerative activity of stem and progenitor cells. Understanding this interaction can lead to the development of more effective regenerative therapies. This review summarizes the effects of immune cell types on endogenous stem and progenitor cells, and discusses regenerative strategies that mimic immune components to enhance the regenerative capacity of both endogenous and transplanted stem cells.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Cell & Tissue Engineering
Amankeldi A. Salybekov, Mehdi Hassanpour, Shuzo Kobayashi, Takayuki Asahara
Summary: Chronic diseases and associated risk factors can negatively affect the function of regenerative cells and the regenerative microenvironment. The vasculogenic conditioning culture (VCC) method has been developed to enhance the regenerative microenvironment and generate regeneration-associated cells (RACs), which consist of vasculogenic endothelial progenitor cells (EPCs) and anti-inflammatory/anti-immunity cells. Both preclinical and clinical studies have shown that RAC transplantation is a safe and effective approach for promoting tissue recovery in ischemic conditions. This comprehensive review highlights the universal features of RACs and the latest advancements in their translation to clinical applications.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell Biology
Cyril Bouland, Pierre Philippart, Didier Dequanter, Florent Corrillon, Isabelle Loeb, Dominique Bron, Laurence Lagneaux, Nathalie Meuleman
Summary: Bone regeneration is a complex process relying on interactions between osteogenesis and angiogenesis. Tissue engineering provides strategies with regenerative cell sources, growth factors, and mechanical stimulation. Vascularization is crucial for bone formation and function, with studies showing synergy between mesenchymal stromal cells (MSCs) and endothelial progenitor cells (EPCs) in promoting regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)