Article
Fisheries
Xiaoyun Zhou, Zexia Gao, Shuangshuang Luo, Junxiao Su, Shaokui Yi
Summary: In this study, the coexistence of diploids and tetraploids in the cyprinid loach was investigated. The tetraploids showed superior growth performance and delayed ovarian development compared to diploids. At the genetic level, numerous differentially expressed genes were identified between diploids and tetraploids, including candidate genes involved in growth and gonad development regulation. The up-regulation of key candidate genes was particularly prominent in the pituitary and gonad of tetraploids. Overall, this study provides valuable genetic evidence for understanding the growth superiority and delayed gonadal development of tetraploids, as well as insights into the genetic superiority of polyploidy species in other phenotypes.
Article
Fisheries
Ziyu Zhou, Gu Shan, Xin Li, Wenxuan Guo, Kexin Ma, Yueyao Yang, Yifan Li, Yunbang Zhang, He Zhou, Xiaojuan Cao
Summary: This study successfully created hybrid triploid loaches with characteristics of fast growth, high-quality meat, high edibility, high disease resistance, and sterility by crossing a natural tetraploid loach and a diploid loach. Through histological observations and transcriptome analyses, 10 differentially expressed genes related to the early fertility mechanism and two signal pathways closely related to the early fertility mechanism were identified. These findings laid a foundation for further research on the molecular inhibition mechanism in hybrid triploid loaches.
Article
Fisheries
Junqing Sheng, Le Guan, Bin Sheng, Shiyu Geng, Di Wu, Beijuan Hu, Zhixiong Li, Shunlong Le, Yijiang Hong
Summary: The study investigated the pigment cell composition and tyrosinase content in three types of loaches, revealing significant differences which may provide a theoretical basis for understanding the mechanism of black spot formation.
JOURNAL OF FISH BIOLOGY
(2022)
Article
Fisheries
Luke P. Oliver, Joseph T. Evavold, Kenneth D. Cain
Summary: Experiments were conducted to optimize triploid induction parameters in burbot, and it was found that hydrostatic shocks and thermal shocks could effectively induce triploidy with high percentages. The survival rates of triploid burbot were statistically similar to the control group. The sterility experiments confirmed that triploid burbot are functionally sterile. These results are significant for the production of sterile burbot.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2023)
Article
Computer Science, Artificial Intelligence
Malliga Subramanian, Kogilavani Shanmugavadivel, P. S. Nandhini
Summary: In this study, the use of deep learning models to identify maize leaf diseases is proposed. The transfer learning of pre-trained models is explored, and VGG16, InceptionV3, and Xception models achieved high accuracy in classifying maize leaf diseases.
NEURAL COMPUTING & APPLICATIONS
(2022)
Article
Fisheries
Vojtech Kaspar, Martin Hubalek, Martin Psenicka, Katsutoshi Arai, John B. Taggart, Roman Franek
Summary: Methods of induction of uniparental inheritance have been developed for model organisms as well as for species of commercial interest, with cold-shock androgenesis showing promise in successfully inducing haploid progeny in common carp. Compared to other techniques, cold-shock androgenesis was found to be effective in common carp, providing a new possibility for isogenic line production.
Article
Fisheries
Roman Franek, Yu Cheng, Michaela Fucikova, Vojtech Kaspar, Xuan Xie, Mujahid Ali Shah, Otomar Linhart, Ivo Sauman, Martin Psenicka
Summary: Surrogate broodstock technology has potential in aquaculture and endangered species conservation. This study investigated the effects of different sterilization strategies on surrogacy using zebrafish as a model species. Germ cell-depleted recipients with normal somatic cells were identified as the most convenient sterilization method considering germline chimera rate and reproductive output.
Article
Anatomy & Morphology
Anna Pospisilova, Jan Stundl, Jindrich Brejcha, Brian D. Metscher, Martin Psenicka, Robert Cerny, Vladimir Soukup
Summary: The study provides a detailed overview of dentition development in the sterlet sturgeon, revealing dynamic changes in tooth composition at different stages of the fish's life. Early loss of oral teeth and subsequent development of palatal and pharyngeal teeth result in a translocation of functional dentition within the oropharyngeal cavity, ultimately leading to the loss of all teeth. These unique dynamics in sturgeons offer valuable insights into the developmental and evolutionary mechanisms of vertebrate odontogenesis.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Cell Biology
Jan Stundl, Vladimir Soukup, Roman Franek, Anna Pospisilova, Viktorie Psutkova, Martin Psenicka, Robert Cerny, Marianne E. Bronner, Daniel Meulemans Medeiros, David Jandzik
Summary: In the last decade, CRISPR/Cas9 has been proven to be an effective and efficient method for targeted mutagenesis in genetic model systems. Its high efficiency allows the immediate generation of near-null mosaic mutants, making it valuable for organisms with life histories that preclude the establishment of purebred genetic lines. In the study, the successful use of CRISPR/Cas9 mutagenesis in a non-teleost fish, sterlet Acipenser ruthenus, was reported, indicating the potential for applying this technology to other long-lived, slow-maturing aquatic vertebrates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Fisheries
Mujahid Ali Shah, Effrosyni Fatira, Viktoriia Iegorova, Xuan Xie, David Gela, Marek Rodina, Roman Franek, Martin Psenicka, Taiju Saito
Summary: The developmental characteristics of sturgeon embryos are similar to those of African clawed frogs. The role of the vegetal pole (VP) in sturgeon embryos is mainly to produce primordial germ cells, while the rest of the cells serve as cellular yolk. The study suggests that meroblastic cleavage in sturgeons might have evolved through the fusion of vegetal blastomeres.
Article
Reproductive Biology
Tomas Tichopad, Roman Franek, Marie Dolezalkova-Kastankova, Dmitrij Dedukh, Anatolie Marta, Karel Halacka, Christoph Steinbach, Karel Janko, Martin Psenicka
Summary: Interspecific hybridization may lead to a transition from sexual reproduction to asexual reproduction, but the reasons for this change are not well understood. In this study, we investigated the triggers of asexual reproduction and found that it is directly influenced by the hybrid genetic constitution of the cell. Additionally, the execution of asexual development is regulated by signals from the surrounding gonadal environment and somatic factors.
BIOLOGY OF REPRODUCTION
(2022)
Article
Genetics & Heredity
Zuzana Blahova, Roman Franek, Marek Let, Martin Blaha, Martin Psenicka, Jan Mraz
Summary: This study used the CRISPR/Cas9 genome editing system to induce mutations in the zebrafish genome and found that fads2 gene editing may result in a decrease in egg quality. The study also revealed a new LC-PUFA biosynthesis pathway in zebrafish.
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Ravindra Naraine, Ievgen Lebeda, Ales Tomcala, Mariola Dietrich, Roman Franek, Martin Psenicka, Radek Sindelka
Summary: This study analyzed the effect of DNA damage on embryo development and found significant changes in the transcriptome and proteome patterns after exposure to genotoxicants. The results suggest a correlation between phenotype formation and changes in transcriptomic and proteomic profiles. Additionally, markers of DNA damage response and adaptive stress response were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Ravindra Naraine, Viktoriia Iegorova, Pavel Abaffy, Roman Franek, Vladimir Soukup, Martin Psenicka, Radek Sindelka
Summary: The study revealed significant differences in RNA localization among different vertebrate models, indicating the highly diverse regulation of embryonic development within the animal kingdom cannot be deduced based on a single model.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Viktoriia Iegorova, Ravindra Naraine, Martin Psenicka, Monika Zelazowska, Radek Sindelka
Summary: The localization of biomolecules within the oocyte drives the spatial differentiation of daughter cells during embryogenesis. While the localization pathways of vegetal maternal transcripts have been well-described, limited information is available about the localization of animal pole transcripts. In this study, TOMO-Seq analysis on two different species was performed, revealing temporal preferences for transcript localization establishment and the presence of both conserved and species-specific transcripts. Additionally, active remodeling of the maternal RNA pool through de novo transcription and degradation was observed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Veterinary Sciences
Xuan Xie, Roman Franek, Martin Psenicka, Fan Chen, Vojtech Kaspar
Summary: This study optimized the cultivation conditions for germ cells in common carp, and the results showed that using hESC media and RTG2 cell line as feeder cells promoted germ cell proliferation and survival. Additionally, opening the carp from the dorsal side minimized the risk of contamination. These findings are important for common carp breeding, genetic resource conservation, and development of specific techniques.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Jelena Lujic, Roman Franek, Zoran Marinovic, Vojtech Kaspar, Xuan Xie, Akos Horvath, Martin Psenicka, Bela Urbanyi
Summary: The aim of this study was to test the efficacy of needle-immersed vitrification (NIV) for preserving oogonia (OOG) in sterlet and Russian sturgeon ovarian tissue in order to enhance conservation efforts for these endangered fish species. The study found that vitrification of the ovarian tissue using specific solutions resulted in viable OOGs, which were able to successfully colonize recipient gonads after transplantation. The simplicity and practicality of the vitrification procedure make it a valuable tool for conservation work in the field.
Article
Veterinary Sciences
Effrosyni Fatira, Milos Havelka, Taiju Saito, Jose Landeira, Marek Rodina, David Gela, Martin Psenicka
Summary: In this study, the researchers applied intracytoplasmic sperm injection (ICSI) technology to endangered sturgeons and found that it successfully produced embryos and larvae. Furthermore, molecular analysis showed genome integration of both egg and sperm donor species, demonstrating the potential of ICSI for assisted reproduction in sturgeons.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Environmental Sciences
Zuzana Weiserova, Jana Blahova, Veronika Dobukova, Petr Marsalek, Nikola Hodkovicova, Jiri Lenz, Frantisek Tichy, Roman Franek, Martin Psenicka, Ales Franc, Zdenka Svobodova
Summary: This study investigated the comprehensive effects of 17 alpha-ethinylestradiol (EE2) in zebrafish, focusing on endocrine disruption, oxidative stress, and detoxification processes. Adult male triploid zebrafish were exposed to two concentrations of EE2 in their feed for six weeks. The high concentration of EE2 caused extensive mortality and significant changes in reproductive disorders, gonad histology, vitellogenin level, and selected gene expression. The environmentally relevant concentration of EE2 showed no significant differences in vitellogenin but did affect antioxidant and detoxifying enzymes. This is the first study to report the effects of subchronic oral exposure to EE2 in adult triploid zebrafish.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Jan Stundl, Megan L. Martik, Donglei Chen, Desingu Ayyappa Raja, Roman Franek, Anna Pospisilova, Martin Psenicka, Brian D. Metscher, Ingo Braasch, Tatjana Haitina, Robert Cerny, Per E. Ahlberg, Marianne E. Bronner
Summary: Neural crest cells in ray-finned fish can differentiate into osteoblasts that form the bony part of the dermal armor, and these cells retain a neural crest gene signature. This discovery is significant for understanding the origin and evolution of skeletal structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
