Article
Biochemical Research Methods
Xinyu Guo, Ruixue Wang, Ruifang Ma, Yan Gao, Xiangyang Zhang, Zhiguang Yuchi, Hui-Yuan Wu
Summary: This study successfully expressed and purified active recombinant mouse CCP6 in a bacterial system with deglutamylation activity. Despite initial association with Cpn60, the CCP6 obtained after proper treatment exhibited enzyme activity.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Plant Sciences
Imke I. Hoermiller, Dietmar Funck, Lilli Schoenewolf, Henrik May, Arnd G. Heyer
Summary: Proline, an amino acid that accumulates in plants under abiotic stress, plays an important role in freezing tolerance in Arabidopsis thaliana. Mutants with altered proline allocation and localization showed reduced basal freezing tolerance, indicating the significance of cytosolic proline. Additionally, the starch excess 1-1 mutant affected photosynthetic electron transport and increased sensitivity to cold stress.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Aamir S. Mukadam, Lauren V. C. Miller, Annabel E. Smith, Marina Vaysburd, Siri A. Sakya, Sophie Sanford, Sophie Keeling, Benjamin J. Tuck, Taxiarchis Katsinelos, Chris Green, Lise Skov, Sanne S. Kaalund, Stian Foss, Keith Mayes, Kevin 'Connell, Mark Wing, Claire Knox, Jessica Banbury, Edward Avezov, James B. Rowe, Michel Goedert, Jan Terje Andersen, Leo C. James, William A. McEwan
Summary: Passively transferred antibodies can target and protect against tau pathology in neurodegenerative diseases. The cytosolic Ab receptor and E3 ligase TRIM21 have been identified as potential mechanisms of antibody protection against tau aggregation.
Article
Cell Biology
Hong Sun, Yu Huang, Shan Mei, Fengwen Xu, Xiaoman Liu, Fei Zhao, Lijuan Yin, Di Zhang, Liang Wei, Chao Wu, Shichao Ma, Jianwei Wang, Shan Cen, Chen Liang, Siqi Hu, Fei Guo
Summary: cGAS is localized in both the cytoplasm and nucleus, demonstrating a functional nuclear export signal for its positioning between the nucleus and cytoplasm. Mutation of the key amino acid L172 results in the loss of interferon response to DNA stimulation.
Article
Biochemistry & Molecular Biology
Yu Huang, Weiwei Cai, Qiaoling Lu, Jingang Lv, Meiyun Wan, Deyi Guan, Sheng Yang, Shuilin He
Summary: High temperature stress is a significant abiotic stress for plants, especially solanacaes such as pepper. In this study, PMT6, a putative methyltranferase, was found to interact with SWC4 and confer SWC4 methylation. PMT6 acts as a positive regulator in pepper thermotolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Maria Milagros Giaccagli, Matias Daniel Gomez-Elias, Jael Dafne Herzfeld, Clara Isabel Marin-Briggiler, Patricia Sara Cuasnicu, Debora Juana Cohen, Vanina Gabriela Da Ros
Summary: The study investigated the role of mitochondrial activity in sperm fertilizing ability during capacitation in mice, finding that MMP increase depends on capacitation and H89-sensitive events, high MMP is associated with sperm motility, and mitochondrial function is needed mainly for zona pellucida penetration. Additionally, mitochondrial activity is fundamental for sperm function in fertilization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Huan Wang, Bin Chen, Juan Tian, Zhaosheng Kong
Summary: The study reveals that VdBre1 plays a crucial role in regulating the infection and pathogenicity of Verticillium dahliae on cotton by globally modulating lipid metabolism and secondary metabolism, impacting plant resistance and spore growth.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiangli Zhao, Ronghan Liu, Yuehong Chen, Aubryanna Hettinghouse, Chuanju Liu
Summary: Fexofenadine, a commonly used antihistamine, has been found to be therapeutic in murine models of chemically-induced IBD, with its anti-inflammatory effects depending on cPLA2 rather than histamine H1 receptor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Feng Xiong, Xiuliang Zhu, Changsha Luo, Zhixiang Liu, Zengyan Zhang
Summary: This study identified a cytosolic AACT-encoding gene, TaAACT1, in wheat and demonstrated its positive regulatory role in wheat defense against F. pseudograminearum. The transcript level of TaAACT1 was higher in FCR-resistant wheat genotypes compared to susceptible genotypes after infection. Knocking down of TaAACT1 impaired resistance against F. pseudograminearum and reduced the expression of defense genes in wheat. This research sheds light on the molecular mechanisms underlying wheat defense against FCR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Eun-Ji Park, Hyun-Soo Kim, Do-Hyoung Lee, Su-Min Kim, Joon-Sup Yoon, Ji-Min Lee, Se Jin Im, Ho Lee, Min-Woo Lee, Chang-Woo Lee
Summary: This study demonstrates the important role of the cold-responsive phosphatase Ssu72 in mRNA translation and thermogenic adaptation in brown adipocytes. Ssu72 deficiency leads to mitochondrial dysfunction and impaired thermogenesis in BAT, which can be rescued by restoring Ssu72 expression.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Amy E. Loats, Samantha Carrera, Anna F. Fleming, Abigail R. E. Roberts, Alice Sherrard, Eneda Toska, Alexander J. Moorhouse, Kathryn F. Medler, Stefan G. E. Roberts
Summary: The study reveals that the lipidated protein BASP1 recruits cholesterol to the promoter region of target genes in the cell nucleus through a conserved interaction motif, and that BASP1's interaction with cholesterol is required for chromatin remodeling and the direction of transcription programs controlling cell differentiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Jingya Xu, Xiaofei Li, Wei Chen, Ziyin Zhang, Yanping Zhou, Yahui Gou, Cheng-an Lv, Lu Jin, Xinyuan Qiu, Shengshan Ma, Qing-Qian Wu, Tongyu Liu, Lin Mi, Zhuoying Yang, Ting Yu, Xiaowen Pan, Yu Feng, Pengfei Shan, Zhuo-Xian Meng
Summary: Xu et al. have demonstrated that Baf60c plays a critical role in muscle regeneration by mediating paracrine signaling through Dkk3 in skeletal myofibers. They found that Baf60c interacts with Six4 to suppress Dkk3 expression in myocytes. This study also reveals the downregulation of Baf60c in obese and T2D individuals, and the upregulation of Dkk3 in muscle-specific Baf60c-deficient mice, leading to impaired muscle regeneration.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Karen Claesen, Joni De Loose, Pieter Van Wielendaele, Emilie De Bruyn, Yani Sim, Sofie Thys, Ingrid De Meester, Dirk Hendriks
Summary: Carboxypeptidase U (CPU) is synthesized in the liver as its inactive precursor proCPU and acts as a regulator of fibrinolysis and inflammation. Monocytes and macrophages, which play a central role in inflammation and thrombus formation, were found to express proCPU and secrete CPU. The expression and secretion of proCPU in these cells are related to their differentiation degree.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
ShengPeng Wang, Bianbian Wang, Yue Shi, Tanja Moeller, Rebekka Stegmeyer, Boris Strilic, Ting Li, Zuyi Yuan, Changhe Wang, Nina Wettschureck, Dietmar Vestweber, Stefan Offermanns
Summary: The extravasation of leukocytes, a critical step in inflammation, requires the close interaction between leukocytes and adhesion molecules on the endothelial cells. A recent study shows that mechanical forces generated by leukocyte-induced clustering of adhesion molecule ICAM-1 synergize with fluid shear stress from flowing blood to activate the mechanosensitive cation channel PIEZO1 on the endothelial plasma membrane. This leads to downstream signaling events that result in the opening of the endothelial barrier and facilitates leukocyte diapedesis.
Article
Multidisciplinary Sciences
Che A. Stafford, Alicia-Marie Gassauer, Carina C. de Oliveira Mann, Maria C. Tanzer, Evelyn Fessler, Benedikt Wefers, Dennis Nagl, Gunnar Kuut, Karolina Sulek, Catherine Vasilopoulou, Sophia J. Schwojer, Andreas Wiest, Marie K. Pfautsch, Wolfgang Wurst, Monica Yabal, Thomas Frohlich, Matthias Mann, Nicolas Gisch, Lucas T. Jae, Veit Hornung
Summary: NAGK plays a critical role in the immunostimulatory activity of MDP by directly phosphorylating the N-acetylmuramic acid moiety of MDP to trigger the functionality of NOD2. This finding reveals a link between amino sugar metabolism and innate immunity to bacterial cell walls.
Article
Critical Care Medicine
Changfu Yao, Xiangrong Guan, Gianni Carraro, Tanyalak Parimon, Xue Liu, Guanling Huang, Apoorva Mulay, Harmik J. Soukiasian, Gregory David, Stephen S. Weigt, John A. Belperio, Peter Chen, Dianhua Jiang, Paul W. Noble, Barry R. Stripp
Summary: The study demonstrates that senescence of AT2 cells is sufficient to drive progressive pulmonary fibrosis, and interventions targeting p53 activation or senescence can block fibrogenesis. Early attenuation of senescence-related pathways and elimination of senescent cells are promising therapeutic approaches to prevent pulmonary fibrosis.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Immunology
Benyu Liu, Nian Liu, Xiaoxiao Zhu, Liuliu Yang, Buqing Ye, Huimu Li, Pingping Zhu, Tiankun Lu, Yong Tian, Zusen Fan
Summary: The newly identified circular RNA circZbtb20 is crucial for the maintenance and function of group 3 innate lymphoid cells (ILC3), with its deletion causing reduced ILC3 numbers and increased susceptibility to bacterial infections. The circZbtb20 enhances the stability of specific mRNA and initiates a signaling pathway to maintain the homeostasis of ILC3.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Miriam S. Hohmann, David M. Habiel, Milena S. Espindola, Guanling Huang, Isabelle Jones, Rohan Narayanan, Ana Lucia Coelho, Justin M. Oldham, Imre Noth, Shwu-Fan Ma, Adrianne Kurkciyan, Jonathan L. McQualter, Gianni Carraro, Barry Stripp, Peter Chen, Dianhua Jiang, Paul W. Noble, William Parks, John Woronicz, Geoffrey Yarranton, Lynne A. Murray, Cory M. Hogaboam
Summary: IPF is characterized by elevated levels of CCR10 and CCL28 in lung tissue, with CCR10(+) cells promoting pulmonary fibrosis and EphA3 mAb targeting these cells reducing fibrosis.
Article
Multidisciplinary Sciences
Xue Liu, Simon C. Rowan, Jiurong Liang, Changfu Yao, Guanling Huang, Nan Deng, Ting Xie, Di Wu, Yizhou Wang, Ankita Burman, Tanyalak Parimon, Zea Borok, Peter Chen, William C. Parks, Cory M. Hogaboam, S. Samuel Weigt, John Belperio, Barry R. Stripp, Paul W. Noble, Dianhua Jiang
Summary: Pulmonary mesenchymal cells play critical roles in lung development and disease states in both mice and humans, showing high heterogeneity and subtype-specific transcriptomic differences which contribute to matrix gene expression in fibrosis. Comparative analysis reveals conserved transcriptomic signatures in orthologous subpopulations of murine and human lung mesenchymal cells, enhancing our understanding of mesenchymal cell diversity in lung development and disease conditions.
Article
Cell Biology
Ruibao Su, Li-Hua Fan, Changchang Cao, Lei Wang, Zongchang Du, Zhaokui Cai, Ying-Chun Ouyang, Yue Wang, Qian Zhou, Ligang Wu, Nan Zhang, Xiaoxiao Zhu, Wen-Long Lei, Hailian Zhao, Yong Tian, Shunmin He, Catherine C. L. Wong, Qing-Yuan Sun, Yuanchao Xue
Summary: The LACE-seq method developed by Xue and colleagues allows for comprehensive profiling of RNA-binding protein targets at single-nucleotide resolution in low-input cells, shedding light on the essential functions of RBPs in germline and early embryo development. The precise mapping of RBP-binding sites in low-input cells opens up new avenues for studying the roles of RBPs in embryonic development and reproductive diseases.
NATURE CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ting Xie, Vrishika Kulur, Ningshan Liu, Nan Deng, Yizhou Wang, Simon Coyle Rowan, Changfu Yao, Guanling Huang, Xue Liu, Forough Taghavifar, Jiurong Liang, Cory Hogaboam, Barry Stripp, Peter Chen, Dianhua Jiang, Paul W. Noble
Summary: Recent studies have shown that impaired renewal of type 2 alveolar epithelial cells (ATII) in IPF patients and mice with defective ATII can lead to severe pulmonary fibrosis. Growth hormone receptor (GHR) expression, mainly found in mesenchymal cells, is significantly decreased in IPF lungs, but higher levels of GHR expression are associated with better lung function in IPF patients. The findings suggest that a previously unidentified mesenchymal paracrine signaling via GHR can support ATII progenitor cell renewal and limit the severity of lung fibrosis.
Article
Biochemistry & Molecular Biology
Song Qiu, Zhenyu Guo, Faiza Naz, Zhao Yang, Changyuan Yu
Summary: This review discusses the utilization, development, and cost of cathode materials in MFCs, highlighting effective modification methods that have led to improved power generation in recent years.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yang Gu, Yanying Wang, Luyun He, Jiahang Zhang, Xiaoxiao Zhu, Nian Liu, Jianyi Wang, Tiankun Lu, Lei He, Yong Tian, Zusen Fan
Summary: This study identified circIPO11 as highly expressed in HCC tumor tissues and liver CSCs, playing a critical role in self-renewal maintenance of liver CSCs and HCC development. Mechanistically, circIPO11 activates the Hedgehog signaling pathway, promoting tumor cell propagation. Targeting circIPO11 and the Hedgehog pathway may provide new potential for HCC treatment.
Article
Biochemistry & Molecular Biology
Guoxu Song, Fei Zhang, Chunhong Tian, Xing Gao, Xiaoxiao Zhu, Dongdong Fan, Yong Tian
Summary: This study identified novel anti-CRISPR families that have the ability to inhibit Cas9 activity and even control genome editing post-translationally. By studying different Acr variants and developing chemically inducible control, new avenues for CRISPR-Cas9 applications have been established.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yanying Wang, Jing Wang, Xiaoyu Li, Xushen Xiong, Jianyi Wang, Ziheng Zhou, Xiaoxiao Zhu, Yang Gu, Dan Dominissini, Lei He, Yong Tian, Chengqi Yi, Zusen Fan
Summary: The study reveals elevated m(1)A methylation levels in hepatocellular carcinoma (HCC) patient tumour tissues and liver cancer stem cells, mediated by the TRMT6/TRMT61A complex. The inhibition of m(1)A methylation can decrease tumourigenesis.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Zhen Xiong, Xiaoxiao Zhu, Jingjing Geng, Yuwei Xu, Runyuan Wu, Cunzhen Li, Dongdong Fan, Xiwen Qin, Ying Du, Yong Tian, Zusen Fan
Summary: This study surveyed the acceptance and concerns regarding mRNA vaccines among participants from the United States and China, revealing differences in attitudes between the two countries. The study suggests strengthening public awareness and education to enhance people's knowledge and acceptance of mRNA vaccines.
Article
Medicine, Research & Experimental
Jiurong Liang, Guanling Huang, Xue Liu, Forough Taghavifar, Ningshan Liu, Yizhou Wang, Nan Deng, Changfu Yao, Ting Xie, Vrishika Kulur, Kristy Dai, Ankita Burman, Simon C. Rowan, S. Samuel Weigt, John Belperio, Barry Stripp, William C. Parks, Dianhua Jiang, Paul W. Noble
Summary: This study identified a deficiency of the zinc transporter SLC39A8 (ZIP8) in Type 2 alveolar epithelial cells (AEC2s) from both idiopathic pulmonary fibrosis (IPF) lungs and lungs of old mice, which is associated with impaired AEC2 renewal capacity and enhanced lung fibrosis. The regulation of AEC2 progenitor function by ZIP8 is dependent on SIRT1. Replenishment with exogenous zinc and activation of SIRT1 promote self-renewal and differentiation of AEC2s, and therapeutic strategies targeting zinc metabolism and SIRT1 signaling could potentially improve AEC2 progenitor function and mitigate fibrogenesis.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Cell Biology
Pingping Zhu, Tiankun Lu, Jiayi Wu, Dongdong Fan, Benyu Liu, Xiaoxiao Zhu, Hui Guo, Ying Du, Feng Liu, Yong Tian, Zusen Fan
Summary: This study reveals how microbiota and the intestinal environment coordinately regulate intestinal stem cells through the activation of enteric serotonergic neurons and the promotion of specific signaling pathways between cells. These findings provide a new perspective for understanding the regulation mechanism of intestinal stem cells.
Article
Immunology
Buqing Ye, Liuliu Yang, Benyu Liu, Nian Liu, Dongdong Fan, Huimu Li, Lei Sun, Ying Du, Shuo Wang, Yong Tian, Zusen Fan
Summary: This study demonstrates that thymidine can induce transdifferentiation of mouse fibroblasts into neutrophils, which exhibit antibacterial effects and can cure neutropenia in mice.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Review
Oncology
Faiza Naz, Mengran Shi, Salvia Sajid, Zhao Yang, Changyuan Yu
Summary: Cancer is characterized by heterogeneity, primarily caused by cancer stem cells that can be transformed by both intrinsic and extrinsic factors to intensify the aggressiveness of cancer cells. Understanding the intrinsic and extrinsic factors that influence cancer stem cell heterogeneity is crucial for intervening in these processes to ultimately improve cancer research and management.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
