Article
Oncology
Ying Shan, Beibei Zhang, Li Chen, Hu Zhang, Cui Jiang, Qinghua You, Yanyi Li, Hongyu Han, Jianlong Zhu
Summary: This study aimed to explore the expression of HVEM in ovarian cancer and its correlation with Tregs. The expression of HVEM in peripheral blood of ovarian cancer patients was detected and the ratio of Treg cells was determined. Ovarian cancer cell lines with high- and low-HVEM expression were constructed and co-cultured with CD4+ cells. The results showed that HVEM promoted the expression of IL-2 and TGF-beta 1, leading to an increase in Treg cells.
Article
Biochemistry & Molecular Biology
Xiguang Ye, Qilin Lu, Aofei Yang, Jun Rao, Wei Xie, Chengjian He, Weijun Wang, Hao Li, Zhiwen Zhang
Summary: The study found that high expression of miR-206 in patients with OA was positively correlated with peripheral Th17/Treg imbalance. MiR-206 disrupted the Th17/Treg balance by targeting SOCS3 and Foxp3. In vivo experiments showed that antagomiR against miR-206 restored the Th17/Treg balance during the development of OA.
MOLECULAR MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Lin-Xin Zheng, Kai-En Guo, Jia-Qi Huang, Miao-Hua Liu, Bai-Ling Deng, Duan-Yong Liu, Bu-Gao Zhou, Wen Zhou, You-Bao Zhong, Hai-Mei Zhao
Summary: By inhibiting the JAK1/STAT3/SOCS signaling pathway, Cur regulates the homeostasis of mTh/mTfh cells to reduce colonic pathological damage induced by DSS.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Immunology
Fatma Oner, Carla Alvarez, Wael Yaghmoor, Danielle Stephens, Hatice Hasturk, Erhan Firatli, Alpdogan Kantarci
Summary: RvE1 targets T(h)17 activation and DC function to achieve inflammatory resolution and acquired immune response.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Edwin J. Heeregrave, Jordan Thomas, Toni M. van Capel, Esther C. de Jong, Georgios Pollakis, William A. Paxton
Summary: HIV-1 can infect various cell types, and the phenotype of the virus can be influenced by the characteristics of the producing cell. Comparing virus strains produced from different cell types, it was found that virus produced from macrophages is more resistant to 2G12 neutralization and more efficiently transmitted to CD4+ cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Oscar J. Cordero, Carlos Rafael-Vidal, Ruben Varela-Calvino, Cristina Calvino-Sampedro, Beatriz Malvar-Fernandez, Samuel Garcia, Juan E. Vinuela, Jose M. Pego-Reigosa
Summary: Immune system CD4 T-cells with high cell-surface CD26 expression demonstrate anti-tumoral properties, especially when engineered with a chimeric antigen receptor. Different T helper cell subsets show variations in CD26 expression levels, which could impact research on CAR-T cells. The relationship between glycoprotein sCD26 and its enzymatic activity, as well as its correlation with specific T cell subsets, still requires further understanding.
Article
Multidisciplinary Sciences
Katherine A. Freitas, Julia A. Belk, Elena Sotillo, Patrick J. Quinn, Maria C. Ramello, Meena Malipatlolla, Bence Daniel, Katalin Sandor, Dorota Klysz, Jeremy Bjelajac, Peng Xu, Kylie A. Burdsall, Victor Tieu, Vandon T. Duong, Micah G. Donovan, Evan W. Weber, Howard Y. Chang, Robbie G. Majzner, Joaquin M. Espinosa, Ansuman T. Satpathy, Crystal L. Mackall
Summary: This research identified MED12 and CCNC as key genes controlling T cell function through CRISPR knockout screening. Knockout of MED12 gene enhances antitumor activity, prolongs the effector phenotype of CAR-T and TCR-T cells, and promotes the expansion of non-engineered T cells. The study reveals the importance of Mediator in T cell effector programming and provides a new target to enhance the potency of antitumor T cell responses.
Article
Immunology
Pierre Gantner, Supranee Buranapraditkun, Amelie Pagliuzza, Caroline Dufour, Marion Pardons, Julie L. Mitchell, Eugene Kroon, Carlo Sacdalan, Nicha Tulmethakaan, Suteeraporn Pinyakorn, Merlin L. Robb, Nittaya Phanuphak, Jintanat Ananworanich, Denise Hsu, Sandhya Vasan, Lydie Trautmann, Remi Fromentin, Nicolas Chomont
Summary: During early stages of HIV infection, the virus initially targets a small population of proliferating memory CD4+ T cells with high surface expression of CCR5. Productively infected cells exhibit different phenotypes and TCR sequences depending on the stage of infection and location within the body. The TCR repertoire of infected cells is biased towards previously expanded and disseminated clones, suggesting independent infection events. Latent genetically intact proviruses are present early in infection, indicating simultaneous generation of latent infected cells.
Article
Psychology, Biological
Fernando Castillo Diaz, Juliana F. Dalto, Magdalena Pereyra, Jorge H. Medina
Summary: Dopamine neurotransmission mediated by D1R in the VTA region is crucial for the processing of negative-valence memories. Interaction between D1R activation in the VTA region and selected downstream target areas plays a role in the formation and persistence of aversive LTM.
PHYSIOLOGY & BEHAVIOR
(2022)
Review
Immunology
Marco Kunzli, David Masopust
Summary: This review provides updates on our understanding of the biology of memory CD4(+) T cells as well as key technological advances that facilitate their characterization.
Article
Immunology
Miranda L. Curtiss, Alexander F. Rosenberg, Christopher D. Scharer, Betty Mousseau, Natalia A. Ballesteros Benavides, John E. Bradley, Beatriz Leon, Chad Steele, Troy D. Randall, Frances E. Lund
Summary: CHI3L1 is found to play a significant role in allergic diseases based on data from patient cohorts and mouse models. In this study, Chi3l1 was shown to modulate T(H)2 and IL-4-producing T-FH cells, which are crucial for IgE responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Enrique Huanosta-Murillo, Marcela Alcantara-Hernandez, Brenda Hernandez-Rico, Georgina Victoria-Acosta, Patricia Miranda-Cruz, Maria Antonieta Dominguez-Gomez, Fermin Jurado-Santacruz, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Alam Palma-Guzman, Paula Licona-Limon, Ezequiel M. Fuentes-Panana, Alicia Lemini-Lopez, Laura C. Bonifaz
Summary: In cutaneous T cell lymphoma (CTCL), a dominant Th2 profile associated with disease progression has been proposed, with TOX+ CD4(+) T cells producing IL-4(+) in early-stage skin lesions. The NLRP3 receptor plays a role in promoting a Th2 response by controlling IL-4 production, which may have significant implications in disease progression. This mechanism of IL-4 regulation mediated by NLRP3 is amplified in late-stage CTCL, indicating its importance in disease progression.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nana Zhang, Xiaoli Li, Junning Wang, Jinye Wang, Na Li, Yao Wei, Hua Tian, Yahong Ji
Summary: IPAF is a disease characterized by autoimmunity and pulmonary fibrosis, and the deficiency of Gal-9 in Tfh cells may lead to abnormal chronic lung fibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Qinqin Xu, Xiaoling Zhang, Tao Li, Shiying Shao
Summary: This study found that exenatide can restore the Th17/Treg balance in obese diabetic mice by regulating the FoxO1 pathway, thereby improving the progression of diabetes and protecting pancreatic beta-cell function.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Andrew Baessler, Bryce Fuchs, Bryant Perkins, Andrew W. Richens, Camille L. Novis, Malia Harrison-Chau, Linda M. Sircy, Kendall A. Thiede, J. Scott Hale
Summary: This study reveals the important role of Tet2 in CD4+ T cell differentiation and memory formation, which in turn affects the enhancement of immune response. The absence of Tet2 leads to preferential recall of Tfh phenotype in memory cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Masashi Morita, Taro Kaizawa, Taiki Yoda, Takuro Oyama, Reina Asakura, Shun Matsumoto, Yoshinori Nagai, Yasuharu Watanabe, Shiro Watanabe, Hiroshi Kobayashi, Kosuke Kawaguchi, Seiji Yamamoto, Nobuyuki Shimozawa, Takanori So, Tsuneo Imanaka
Summary: The study suggests that bone marrow-derived microglia-like cells engrafted in the cerebrum of X-ALD patients may suppress disease progression without evidently reducing the amount of VLCFA in the cerebrum.
JOURNAL OF INHERITED METABOLIC DISEASE
(2021)
Article
Multidisciplinary Sciences
Kosuke Kawaguchi, Emi Mukai, Shiro Watanabe, Atsushi Yamashita, Masashi Morita, Takanori So, Tsuneo Imanaka
Summary: ABCD1 protein, responsible for transporting very long chain fatty acid-CoA into peroxisomes, possesses both ATPase and ACOT activities, with ACOT activity being essential for the transport process. The detailed mechanism of VLCFA-CoA transport by ABCD1 has been proposed based on experimental results.
SCIENTIFIC REPORTS
(2021)
Article
Gastroenterology & Hepatology
Tomoki Sasaki, Hiroyuki Nagashima, Atsushi Okuma, Takeshi Yamauchi, Kenshi Yamasaki, Setsuya Aiba, Takanori So, Naoto Ishii, Yuji Owada, Takashi MaruYama, Shuhei Kobayashi
Summary: This study found that I kappa B-zeta-deficient mice had more severe gut inflammation induced by DSS, lower levels of IgM in feces, and exacerbated inflammatory responses in the intestine. Antibiotic treatment was able to alleviate this inflammatory phenotype.
DIGESTIVE DISEASES AND SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Tobias Beer, Sebastian Haensch, Klaus Pfeffer, Sander H. J. Smits, Stefanie Weidtkamp-Peters, Lutz Schmitt
Summary: Secretion systems play a vital role in Gram-negative bacteria by allowing communication with the outside world. This study focused on the hemolysin A (HlyA) type I secretion system (T1SS) in Escherichia coli and analyzed its localization and clustering. The results showed that the amount of ToIC, a component of the T1SS, was not affected by overexpression of the HlyBD complex. Superresolution microscopy revealed that the HlyA T1SS did not exhibit polarization and the size of T1SS clusters did not correlate with outer membrane islands.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Immunology
Marc Dalod, Stefanie Scheu
Summary: DCs play a crucial role in antigen presentation and orchestrate information transfer in the immune system. Mouse models provide valuable insights into the different functions of DC types and their activation states. This article provides comprehensive information on selecting mutant mouse strains for DC research and discusses the advantages, limitations, and future perspectives of these models.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Eigo Kawahara, Mitsuki Azuma, Hiroyuki Nagashima, Koki Omori, Sho Akiyama, Yuka Fujimori, Mayu Oishi, Nagito Shibui, Kosuke Kawaguchi, Masashi Morita, Yuko Okuyama, Naoto Ishii, Takanori So
Summary: TRAFS acts as a negative regulator for IL-6 receptor signaling and a positive regulator for GITR-mediated signaling in CD4(+) T cells. It limits the signaling via the IL-27 receptor and promotes the proliferation of CD4(+) T cells. The regulatory activity of TRAF5 in gp130 is distinct from its activity in TNF receptor family molecules in T cells.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Oncology
Jessica M. Salmon, Izabela Todorovski, Kym L. Stanley, Claudia Bruedigam, Conor J. Kearney, Luciano G. Martelotto, Fernando Rossello, Timothy Semple, Gisela Mir Arnau, Magnus Zethoven, Michael Bots, Stefan Bjelosevic, Leonie A. Cluse, Peter J. Fraser, Veronique Litalien, Eva Vidacs, Kate McArthur, Antony Y. Matthews, Elise Gressier, Nicole A. de Weerd, Jens Lichte, Madison J. Kelly, Simon J. Hogg, Paul J. Hertzog, Lev M. Kats, Stephin J. Vervoort, Daniel D. De Carvalho, Stefanie Scheu, Sammy Bedoui, Benjamin T. Kile, Steven W. Lane, Andrew C. Perkins, Andrew H. Wei, Pilar M. Dominguez, Ricky W. Johnstone
Summary: Inhibition of HDACs can induce differentiation and therapeutic effects in AML cells, and epigenetic rewiring of pDCs enhances antitumor immunity, offering a new therapeutic approach.
Article
Immunology
Xiaoli Yang, Shafaqat Ali, Manman Zhao, Lisa Richter, Vanessa Schaefer, Julian Schliehe-Diecks, Marian Frank, Jing Qi, Pia-Katharina Larsen, Jennifer Skerra, Heba Islam, Thorsten Wachtmeister, Christina Alter, Anfei Huang, Sanil Bhatia, Karl Koehrer, Carsten Kirschning, Heike Weighardt, Ulrich Kalinke, Rainer Kalscheuer, Markus Uhrberg, Stefanie Scheu
Summary: This study reveals that BEA activates BMDCs and induces the production of inflammatory cytokines and chemokines, which is dependent on the MyD88 and TRIF signaling pathways as well as TLR4. These findings contribute to a better understanding of the mechanism of action of BEA in immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Naoki Fujitani, Masayoshi Saito, Tomoya Akashi, Masashi Morita, Takanori So, Kozo Oka
Summary: X-linked Adrenoleukodystrophy (X-ALD) is a rare genetic neurological disorder caused by a mutation of the ABCD1 gene. Dysfunction of ABCD1 leads to increased VLCFA levels, but plasma VLCFA levels cannot predict disease progression. Lipids containing VLCFA signals in phosphatidylcholine have the potential to be informative biomarkers for assessing X-ALD pathology.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Immunology
Manfred B. Lutz, Shafaqat Ali, Cindy Audiger, Stella E. Autenrieth, Luciana Berod, Venetia Bigley, Laura Cyran, Marc Dalod, Jan Doerrie, Diana Dudziak, Georgina Florez-Grau, Lucila Giusiano, Gloria J. Godoy, Marion Heuer, Anne B. Krug, Christian H. K. Lehmann, Christian T. Mayer, Shalin H. Naik, Stefanie Scheu, Gerty Schreibelt, Elodie Segura, Kristin Sere, Tim Sparwasser, Jurjen Tel, Huaming Xu, Martin Zenke
Summary: This article is part of a series of guidelines on dendritic cells, providing advanced protocols for the preparation and characterization of murine and human dendritic cells. The protocols cover the preparation and generation of dendritic cells from different cellular sources, including the generation of different subsets such as cDC1, cDC2, and pDC using Flt3L in mice, and the generation of bone marrow-derived dendritic cells using GM-CSF. For human dendritic cells, the protocols focus on the culture of CD34(+) cells on OP9 cell layers to generate different subsets, as well as the generation of dendritic cells from peripheral blood monocytes. The article has been peer-reviewed and approved by the co-authors, making it an essential resource for basic and clinical dendritic cell immunologists.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Infectious Diseases
Flaminia Mazzone, Viktor E. Simons, Lasse van Geelen, Marian Frank, Attila Mandi, Tibor Kurtan, Klaus Pfeffer, Rainer Kalscheuer
Summary: Toxoplasma gondii is a pathogen that can infect various warm-blooded animals, including humans, causing toxoplasmosis. Current treatments for toxoplasmosis have severe side effects and are not effective in eradicating chronic infection. Therefore, there is an urgent need to develop novel anti-toxoplasma drugs with low toxicity. In this study, eight natural products were isolated from the crude extract of the endophytic fungus Paraboeremia selaginellae obtained from the leaves of the plant Philodendron monstera. Six of these natural products showed antitoxoplasma activity with low or no cytotoxicity in human cell lines. These findings suggest that biphenyl ethers, bioxanthracenes, and 5S,6S-phomalactone from P. selaginellae are potential candidates for novel anti-toxoplasma drugs.
Article
Cell Biology
Hodaka Nagai, Mitsuki Azuma, Ayaka Sato, Nagito Shibui, Sayaka Ogawara, Yuta Tsutsui, Ayano Suzuki, Tomomi Wakaizumi, Aya Ito, Shimpei Matsuyama, Masashi Morita, Mari Hikosaka Kuniishi, Naoto Ishii, Takanori So
Summary: In this study, the functional significance of four different TNFRs (OX40, 4-1BB, CD27 and GITR) in T cell responses was investigated. The results showed that each individual Fc-scTNFL protein provides a critical costimulatory signal and exhibits quantitatively distinct activity toward T cells. These findings provide important insights for investigators conducting basic research in cancer immunology and have implications for T cell-mediated immune regulation by designer TNFL proteins.
Editorial Material
Cell Biology
Tukisa D. Smith, Taylor A. Doherty
Summary: Sharma et al. discovered novel heterozygous gain-of-function variants in the STAT6 gene in individuals with severe and early onset multi-systemic allergic disease, contributing to the rapid identification of inborn errors of immunity.
CELL REPORTS MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Ayaka Sato, Mitsuki Azuma, Hodaka Nagai, Wakana Imai, Kosuke Kawaguchi, Masashi Morita, Yuko Okuyama, Naoto Ishii, Takanori So
Summary: This study successfully prepared an OX40 receptor agonist protein that could induce T cell immunity by rational design of OX40L assembly and structure. The protein significantly increased proliferation and cytokine responses in CD4(+) T cells and enhanced T cell-mediated hypersensitivity responses. Therefore, rational design of OX40L can improve T cell responsiveness.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Review
Immunology
Takanori So
Summary: The TRAF family of molecules are intracellular signaling adaptors controlling diverse signal pathways, with dysregulation leading to inflammatory and autoimmune diseases. Understanding TRAF signaling mechanisms is crucial for therapeutic interventions in immune-related disorders.
INTERNATIONAL IMMUNOLOGY
(2022)