Article
Multidisciplinary Sciences
Chao Kong, Xiaozhan Qu, Mingming Liu, Weiya Xu, Da Chen, Yanshen Zhang, Shan Zhang, Feng Zhu, Zhenbang Liu, Jianchao Li, Chengdong Huang, Chao Wang
Summary: The study reveals the dynamic nature of the complex formation between E-cadherin and AnkG and investigates its functional role in maintaining epithelial cell polarity.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, General & Internal
Xuebin Zhang, Yu Duan, Xiao Zhang, Mengyuan Jiang, Wanrong Man, Yan Zhang, Dexi Wu, Jiye Zhang, Xinglong Song, Congye Li, Jie Lin, Dongdong Sun
Summary: Microvascular complications in diabetes patients, including coronary microvascular injury, are associated with a higher risk of adverse outcomes. This study investigated the mechanisms underlying diabetic coronary microvascular hyperpermeability and found that Adipsin plays a vital role in maintaining the integrity of cardiac microvascular endothelial cells.
Article
Critical Care Medicine
Binu Tharakan, Felicia A. Hunter, Saravanakumar Muthusamy, Sonya Randolph, Crystal Byrd, Veena N. Rao, E. Shyam P. Reddy, Ed W. Childs
Summary: This study determined the specific role of ERG in regulating microvascular permeability in human lung microvascular endothelial cells and evaluated the protective effect of exogenous ERG on barrier dysfunction. The results demonstrate that ERG is a potent regulator of barrier integrity and permeability, and enhancing ERG provides protection against barrier dysfunction and hyperpermeability.
Review
Cell Biology
Ece Bayir, Aylin Sendemir
Summary: The blood-brain barrier is a highly selective cellular monolayer unique to the central nervous system microvasculature, mediating communication with the body by regulating the passage of molecules. Recent studies have highlighted the importance of intermediate filaments in the formation and function of cell-cell junctions, particularly vimentin in endothelial cells. Intermediate filaments may have been overlooked as key targets in regulating blood-brain barrier permeability in health and disease.
Article
Immunology
Jiahui Li, Kai Wang, Bo Huang, Rui Li, Xilong Wang, Hailing Zhang, Haixiong Tang, Xin Chen
Summary: The study demonstrated that RAGE signaling mediates airway epithelial barrier dysfunction in a LPS-induced ALI murine model, and inhibition of RAGE effectively alleviates airway inflammation and injury.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dinesh Kankanamge, Sithurandi Ubeysinghe, Mithila Tennakoon, Priyanka Devi Pantula, Kishalay Mitra, Lopamudra Giri, Ajith Karunarathne
Summary: Phospholipase C beta (PLC beta) is activated by the Gq family of heterotrimeric G proteins and plays crucial roles in cellular processes and disease. Signaling crosstalk between Gq and Gi/o pathways influences PIP2 metabolism, with the dissociation of G beta gamma leading to partial recovery of PIP2 levels.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Francesca M. Pramotton, Asra Abukar, Chantelle Hudson, James Dunbar, Andrew Potterton, Simone Tonnicchia, Andrea Taddei, Edoardo Mazza, Costanza Giampietro
Summary: Aging is a major risk factor for chronic diseases, and senescence of endothelial cells is linked to inflammation and diabetic vascular diseases. We identified DYRK1B protein as a potential target for senescent endothelial cells using advanced AI and machine learning techniques, and found that inhibiting DYRK1B can restore endothelial barrier function. Therefore, DYRK1B may be a promising target for combating diabetes-associated vascular diseases linked to endothelial cell senescence.
MECHANISMS OF AGEING AND DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Eoin McEvoy, Tal Sneh, Emad Moeendarbary, Yousef Javanmardi, Nadia Efimova, Changsong Yang, Gloria E. Marino-Bravante, Xingyu Chen, Jorge Escribano, Fabian Spill, Jose Manuel Garcia-Aznar, Ashani T. Weeraratna, Tatyana M. Svitkina, Roger D. Kamm, Vivek B. Shenoy
Summary: This study proposes a chemo-mechanical model to investigate the regulation of endothelial junctions and reveals the influence of cell tension and Rac1 expression on junction stability.
NATURE COMMUNICATIONS
(2022)
Article
Hematology
Pascal Yazbeck, Xavier Cullere, Paul Bennett, Vijay Yajnik, Huan Wang, Kenji Kawada, Vanessa M. Davis, Asit Parikh, Andrew Kuo, Vijayashree Mysore, Timothy Hla, David S. Milstone, Tanya N. Mayadas
Summary: The study reveals that DOCK4, an atypical Rho family GTPase GEF, plays a critical role in lung vascular barrier function by regulating AJs. DOCK4 deficiency leads to lung hemorrhage, increased microvascular permeability, and impaired response to S1P-induced permeability changes. Additionally, S1P may act as a potential upstream regulator of DOCK4 activity.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Neurosciences
Che Chuan Wang, Jinn Rung Kuo, Su Jane Wang
Summary: Fingolimod decreases glutamate release in rat cerebrocortical synaptosomes by suppressing N- and P/Q-type Ca2+ channel activity. The activation of presynaptic S1P1 receptors and the involvement of G protein beta gamma subunit also contribute to this effect.
Article
Pharmacology & Pharmacy
Binu Tharakan, Felicia A. Hunter, Ed W. Childs
Summary: Microvascular hyperpermeability, a serious clinical issue in conditions like shock and sepsis, can be reduced by FK506 treatment through maintenance of mitochondrial transmembrane potential and protection against caspase-3 mediated endothelial cell barrier disruption. Further exploration is needed for the therapeutic potential of FK506 in treating vascular hyperpermeability and its complications.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2021)
Article
Cell Biology
Avtar S. Meena, Pradeep K. Shukla, Briar Bell, Francesco Giorgianni, Rebeca Caires, Carlos Fernandez-Pena, Sarka Beranova, Eitaro Aihara, Marshall H. Montrose, Mehdi Chaib, Liza Makowski, Indira Neeli, Marko Z. Radic, Valeria Vasquez, Jonathan H. Jaggar, Julio F. Cordero-Morales, RadhaKrishna Rao
Summary: Intestinal epithelial tight junction disruption is a key factor in alcohol-induced endotoxemia, systemic inflammation, and organ damage. TRPV6, a Ca2+-permeable channel, is responsible for the elevation of intracellular Ca2+, intestinal barrier dysfunction, and systemic inflammation induced by alcohol. Inhibiting or deficient TRPV6 can attenuate alcohol-induced barrier dysfunction and Ca2+ influx.
Article
Multidisciplinary Sciences
Caterina Ivaldo, Mario Passalacqua, Anna Lisa Furfaro, Cristina d'Abramo, Santiago Ruiz, Prodyot K. Chatterjee, Christine N. Metz, Mariapaola Nitti, Philippe Marambaud
Summary: Oxidative stress induced efficient proteolysis of VE-cadherin by MMPs and gamma-secretase in HUVECs. The cytoplasmic domain of VE-cadherin produced by gamma-secretase, VE-Cad/CTF2, was tightly regulated and sequentially produced from an ADAM10/17-generated C-terminal fragment. BMP9 and BMP10 reduced VE-Cad/CTF2 levels and mitigated H2O2-mediated actin cytoskeleton disassembly.
SCIENTIFIC REPORTS
(2023)
Article
Gastroenterology & Hepatology
Hsuan-Ju Lai, Ha T. Doan, Elliot Y. Lin, Yi-Ling Chiu, Yuan-Kai Cheng, Yi-He Lin, Hao-Sen Chiang
Summary: This study found that in the inflamed colon of patients with inflammatory bowel disease, there is increased formation of neutrophil extracellular traps (NETs) and abundant NET-associated proteins. Using a mouse model of inflammatory bowel disease, it was discovered that PAD4-dependent NET formation has detrimental effects in acute colitis. Furthermore, histones associated with NETs directly affect intestinal barrier function and induce cell death in the intestinal epithelium, leading to increased permeability of the gut epithelium.
INFLAMMATORY BOWEL DISEASES
(2023)
Article
Engineering, Environmental
Mengni Ge, Zhuohui Jia, Quanguo Jiang, Guobing Ying, Yan Yang, Shaoyu Wu, Takashi Goto, Jianfeng Zhang
Summary: This study developed highly-permeable and antifouling thin-film nanocomposite membranes by incorporating graphitic carbon nitride nanomaterials into polyamide layer. The resulting membranes showed improved water permeability and rejection rate compared to traditional polyamide membranes, demonstrating the effectiveness of the modification approach.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Cardiac & Cardiovascular Systems
Jelmer Westra, Ashkan Eftekhari, Mick Renkens, Hernan Mejia-Renteria, Martin Sejr-Hansen, Valerie Stegehuis, Niels Ramsing Holm, Robert-Jan De Winter, Jan J. Piek, Javier Escaned, J. J. Wykrzykowska, Evald Hoj Christiansen
Summary: The physiological and anatomical factors contribute to the disagreement between fractional flow reserve (FFR) and quantitative flow ratio (QFR) in intermediate stenosed epicardial coronary arteries.
INTERNATIONAL JOURNAL OF CARDIOVASCULAR IMAGING
(2022)
Article
Medicine, Research & Experimental
Timothy R. Deer, Marc A. Russo, Jay S. Grider, Jason Pope, Philippe Rigoard, Jonathan M. Hagedorn, Ramana Naidu, Denis G. Patterson, Derron Wilson, Timothy R. Lubenow, Asokumar Buvanendran, Samir J. Sheth, Rany Abdallah, N. Nick Knezevic, Stefan Schu, Harold Nijhuis, Pankaj Mehta, Ricardo Vallejo, Jay M. Shah, Michael E. Harned, Navdeep Jassal, Jose Manuel Gonzalez, Thomas P. Pittelkow, Shachi Patel, Stana Bojanic, Kenneth Chapman, Natalie Strand, Alexander L. Green, Peter Pahapill, Alessandro Dario, Fabian Piedimonte, Robert M. Levy
Summary: Neurostimulation for chronic pain treatment is rapidly advancing, with surgical planning, device placement, and postoperative care being equally important as technological improvements. The NACC project aims to provide evidence-based guidance for these often-overlooked areas in neurostimulation practice, with recommendations based on evidence strength and consensus when evidence is lacking.
Review
Clinical Neurology
Iulia Pirvulescu, Alexandras Biskis, Kenneth D. Candido, Nebojsa Nick Knezevic
Summary: This narrative review discusses and summarizes the effectiveness of prospective research on refractory neuropathic pain (ReNP) conducted over the past 10 years. The review focuses on pharmacological and interventional therapies and highlights the limitations of single drug therapy and the potential of interventional approaches.
EXPERT REVIEW OF NEUROTHERAPEUTICS
(2022)
Review
Anesthesiology
Nebojsa Nick Knezevic, Anthony Nader, Iulia Pirvulescu, Aby Pynadath, Behnoosh B. Rahavard, Kenneth D. Candido
Summary: This article reviews the nature and mechanisms of diurnal variations in pain. It found that pain follows a circadian pattern, with different types of pain showing different expressions along this rhythm. The understanding of diurnal variation in pain may help improve therapeutic strategies in pain management.
Review
Biochemistry & Molecular Biology
Onella Athnaiel, Charmaine Ong, Nebojsa Nick Knezevic
Summary: Chronic pain and depression are global issues affecting millions of people, and their comorbidity exacerbates the severity of each condition. Recent studies have found that inflammatory reactions in these disorders affect enzymes in the kynurenine pathway, leading to the production of specific metabolites that contribute to the pathophysiology of these diseases.
Letter
Anesthesiology
Artush Grigoryan, Arjang Khorasani, M. Ramez Salem, Nebojsa Nick Knezevic
Review
Biochemistry & Molecular Biology
Onella Athnaiel, Santiago Cantillo, Stephania Paredes, Nebojsa Nick Knezevic
Summary: This article examines the impact of hormone replacement therapy on pain perception in the transgender community, particularly in relation to other pain-related conditions such as headaches, fibromyalgia, temporomandibular myalgia, and visceral pain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Clinical Neurology
Laxmaiah Manchikanti, Nebojsa Nick Knezevic, Emilija Knezevic, Salahadin Abdi, Mahendra R. Sanapati, Amol Soin, Bradley W. Wargo, Annu Navani, Sairam Atluri, Christopher G. Gharibo, Thomas T. Simopoulos, Radomir Kosanovic, Alaa Abd-Elsayed, Alan D. Kaye, Joshua A. Hirsch
Summary: This systematic review and meta-analysis examines the effectiveness of radiofrequency neurotomy as a therapeutic intervention for chronic neck pain and cervicogenic headaches. The analysis reveals that radiofrequency neurotomy shows level II evidence in managing chronic neck pain, with moderate to high clinical applicability. However, the evidence is level III to IV in managing cervicogenic headaches, suggesting a relatively lower efficacy.
Review
Cell Biology
Filip Jovanovic, Visnja Jovanovic, Nebojsa Nick Knezevic
Summary: The development of chronic pain involves complex interactions among the nervous, immune, and endocrine systems. Chronic pain, lasting for more than 3 months, is increasingly common in the US adult population. Pro-inflammatory cytokines contribute to chronic pain and affect the tryptophan metabolism and the hypothalamic-pituitary-adrenal axis. Glucocorticoids and metabolites of the kynurenine pathway may serve as biomarkers and potential therapeutic targets for chronic pain.
Review
Chemistry, Medicinal
Osman Syed, Predrag Jancic, Nebojsa Nick Knezevic
Summary: Diabetic peripheral neuropathy is a common complication of diabetes mellitus, and its prevalence is increasing due to the rising number of diabetes cases. This condition has a significant impact on both society and the economy, as patients require ongoing medication and may experience a decline in quality of life. Various pharmacological interventions have been explored, including serotonin norepinephrine reuptake inhibitors, gapentanoids, sodium channel blockers, and tricyclic antidepressants. This article discusses these medications and their respective efficacies, as well as the potential use of glucagon-like peptide-1 agonists in the treatment of peripheral diabetic neuropathy.
Review
Chemistry, Medicinal
Andrew Auyeung, Hank C. Wang, Kannan Aravagiri, Nebojsa Nick Knezevic
Summary: Chronic pain is a significant medical and socioeconomic issue with detrimental effects on individual patients and society. The kynurenine pathway has emerged as a potential contributor to chronic pain, with dysregulation and changes in metabolites associated with neurotoxic and inflammatory states. Further research on biomarkers and receptors involved in this pathway could lead to novel personalized treatments.
Review
Chemistry, Medicinal
Osman Syed, Predrag Jancic, Adam B. Fink, Nebojsa Nick Knezevic
Summary: This article discusses the increasing demand for pharmacological treatment and relief of chronic pain, as well as the safety concerns associated with these treatment options. The psychological impact of these medications, including the risk of suicidality, is highlighted, underscoring the importance of surveillance programs.
Article
Clinical Neurology
Adam B. Fink, Charmaine Ong, Moez K. Sumar, Neil C. Patel, Nebojsa Nick Knezevic
Summary: Two derivative scales, PRPPR and CPPR, commonly used in pain assessment, have rarely been compared. This study examined the agreement between PRPPR and CPPR in chronic pain patients for the first time, as well as the impact of treatment duration on their correlation.
NEUROLOGY INTERNATIONAL
(2023)
Review
Cell Biology
Emilija Knezevic, Katarina Nenic, Vladislav Milanovic, Nebojsa Nick Knezevic
Summary: Cortisol, a crucial hormone in the body, is regulated by various factors and plays a vital role in stress response and maintaining homeostasis. Animal models have been instrumental in understanding the complex dynamics of cortisol during stress and the interplay with other physiological factors. Understanding these mechanisms is essential for mitigating the detrimental consequences of chronic stress and cortisol dysregulation in various conditions.
Review
Anesthesiology
Laxmaiah Manchikanti, Emilija Knezevic, Richard E. Latchaw, Nebojsa Nick Knezevic, Salahadin Abdi, Mahendra R. Sanapati, Peter S. Staats, Christopher G. Gharibo, Thomas T. Simopoulos, Shalini Shah, Alaa Abd-Elsayed, Annu Navani, Alan D. Kaye, Sheri L. Albers, Joshua A. Hirsch
Summary: A reanalysis of the Cochrane Review data suggests that epidural injections are effective in managing chronic low back and lower extremity pain. However, there are conflicting views on the effectiveness of epidural injections in different systematic reviews and meta-analyses.
