期刊
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
卷 399, 期 2, 页码 156-161出版社
ELSEVIER
DOI: 10.1016/j.jembe.2011.01.006
关键词
Algae; Antarctic; Chlorophyll fluorescence; Imaging PAM; Salinity; Sea ice
资金
- New Zealand Foundation for Research Science and Technology [VICX0219, VICX0706]
- Victoria University of Wellington
Bottom-ice algae within Antarctic sea ice were examined using chlorophyll fluorescence imaging. The detailed structure of the bottom-ice algal community growing in the platelet and congelation layers of solid pieces of sea ice was evident for the first time in chlorophyll imaging mode. Strands of fluorescence representing algal cells were clearly visible growing upward into brine channels in a fine network. Images of effective quantum yield (Phi(PSII)) revealed that the Phi(PSII) of algae embedded in the sea ice was approximately 0.5. Furthermore, Phi(PSII) decreased slightly with distance from the ice-water interface. The response of Antarctic sea ice algae to changes in irradiance and salinity, and the effects of slowly warming and melting the ice block sample were examined using this system. The Phi(PSII) of bottom-ice algae decreased as irradiance increased and salinities decreased. Bottom-ice algae appear to be most vulnerable to changes in their environment during the melting process of the ice, and this suggests that algae from this region of the ice may not be able to cope with the stress of melting during summer. Chlorophyll fluorescence imaging provides unprecedented imagery of chlorophyll distribution in sea ice and allows measurement of the responses of sea ice algae to environmental stresses with minimal disruption to their physical habitat. The results obtained with this method are comparable to those obtained with algae that have been melted into liquid culture and this indicates that previous melting protocols reveal meaningful data. In this chlorophyll imaging study, rapid light curves did not saturate and this may prevent further use of this configuration. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据