Article
Plant Sciences
Rui Tian, Xiaohan Sun, Cuimei Liu, Jinfang Chu, Mingui Zhao, Wen-Hao Zhang
Summary: Increasing evidence suggests that lncRNAs play a role in regulating plant tolerance to abiotic stress. This study characterized a salt stress-responsive lncRNA, MtCIR1, from M. truncatula and found that it negatively regulates salt stress response by affecting ABA metabolism and signaling during seed germination and Na+ transport under salt stress during seedling growth in Arabidopsis thaliana. These findings advance our understanding of the regulatory roles of lncRNAs in plant response to salt stress.
Article
Biochemistry & Molecular Biology
Xuemei Zhang, Bowen Tan, Zihan Cheng, Dan Zhu, Tingbo Jiang, Sixue Chen
Summary: The McHB7 gene in ice plant enhances the salt tolerance by regulating the changes in proteins and metabolites, resulting in increased photosynthesis efficiency and chlorophyll content. Additionally, McHB7 also modulates the expression of stress-related proteins to scavenge reactive oxygen species and enhance plant salt tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Junmei Cui, Yang Hu, Yan Huang, Jinya Guo, Xiulan Xie, Huaiyu Zhang, Yi Cai
Summary: Research indicates that IOS1 plays an important and positive regulatory role in salt stress response in Arabidopsis. Loss-of-function ios1 mutants display increased sensitivity to salinity, higher accumulation of H2O2 under salt stress, altered expression of stress marker genes, and weakened phosphorylation of MPK6 compared to wild-type plants.
PLANT MOLECULAR BIOLOGY REPORTER
(2022)
Review
Plant Sciences
Xin Wang, Setsuko Komatsu
Summary: Climate change poses a threat to soybean production through reduced seed yield and quality. This review summarizes the morphophysiological changes in soybeans under abiotic stress and discusses cellular metabolisms and regulatory mechanisms in response to organellar stress using subcellular proteomics. The communication pathways involving reactive oxygen species scavenging, molecular chaperones, and phytohormone signals among subcellular compartments are highlighted. Given the complexity of climate change and plant limitations in coping with multiple abiotic stresses, a generic response involving calcium and abscisic acid signals in subcellular organelles is proposed. This review provides an overview of subcellular proteomics research on stressed soybeans and discusses the future potential of subcellular proteomics in enhancing climate-tolerant crops.
Article
Agriculture, Multidisciplinary
Yixin An, Ziqi Wang, Baijian Liu, Yuwei Cao, Lin Chen
Summary: This study investigates the translation regulation of plant genes under salt stress and finds that salt stress inhibits gene translation efficiency. It also identifies various small ORFs in the genome of Medicago truncatula, which play a role in the translation regulation of genes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Laura Lamelas, Luis Valledor, Cristina Lopez-Hidalgo, Maria Jesus Canal, Monica Meijon
Summary: This study revealed the responses of chloroplasts and nuclei in Pinus radiata under increased temperatures, including impairment of photosynthesis, DNA damage response in the nucleus, and altered chromatin conformation. The study also found proteins transferring from chloroplasts to nuclei carrying stress messages, as well as deep rearrangement of RNA metabolism in both organelles. Overall, the research highlights the coordination among different stages required for thermotolerance acquisition in P. radiata, emphasizing the role of chromatin conformation and posttranscriptional gene regulation in overcoming heat stress for plant survival.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Dirk F. Moore, David E. Sleat, Peter Lobel
Summary: Knowledge of cellular location is crucial for understanding protein function. Subcellular fractionation followed by mass spectrometry is a commonly used method for assigning cellular locations to proteins. However, proteins can reside in multiple compartments, which requires a more accurate method for localization. In this article, the constrained proportional assignment (CPA) method is introduced, along with data transformations and R-based programs for analyzing subcellular proteomics data.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Lei Feng, Yan Li, Yu-Ling Zhou, Guang-Hua Meng, Zhao-Lin Ji, Wen-Hui Lin, Jun-Xian He
Summary: This study investigates the molecular mechanisms of plant responses to salt stress in salt cress. Phenotypic analysis, transcriptomic and proteomic profiling, and other assays were conducted to validate the role of BRs in regulating plant responses to salt stress. The results indicate that BES1 plays a positive role in plant salt tolerance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Shuwei Dong, Lijun Sang, Hongli Xie, Maofeng Chai, Zeng-Yu Wang
Summary: This study investigated the impact of salt stress on leaf senescence in Medicago truncatula, identifying numerous senescence-associated genes (SAGs) at different time points and linking them to processes such as protein metabolism, photosynthesis, chlorophyll metabolism, and hormone signaling. Additionally, various transcription factor families, including NAC, bHLH, MYB, and ERF, were found to be associated with salt stress-induced leaf senescence, providing new insights for further genetic research and breeding in forage crops.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Shilei Luo, Zeci Liu, Zilong Wan, Xianxia He, Jian Lv, Jihua Yu, Guobin Zhang
Summary: Hydrogen sulfide (H2S) is a potential signaling molecule involved in the regulation of plant salt stress. This study explored the effects of H2S on cucumber growth, photosynthesis, antioxidation, ion balance, and salt tolerance pathways. The results showed that NaCl inhibited cucumber seedlings' growth and photosynthesis, while exogenous spraying of sodium hydrosulfide (NaHS) under salt stress maintained growth, increased photosynthesis, enhanced the ascorbate-glutathione cycle (AsA-GSH), and promoted mineral ion absorption. H2S also upregulated specific genes to maintain ion balance and enhance salt tolerance. These effects could be reversed by a specific H2S biosynthesis inhibitor. Therefore, H2S could mitigate salt damage in cucumber through multiple mechanisms.
Article
Plant Sciences
Chrystalla Antoniou, Xavier Zarza, Gholamreza Gohari, Sima Panahirad, Panagiota Filippou, Antonio F. Tiburcio, Vasileios Fotopoulos
Summary: The study revealed a clear correlation between the alteration of polyamine metabolic pathway under salinity conditions and sensitivity in different Medicago genotypes.
Review
Biochemistry & Molecular Biology
Xuexue Chen, Yanglin Ding, Yongqing Yang, Chunpeng Song, Baoshan Wang, Shuhua Yang, Yan Guo, Zhizhong Gong
Summary: Protein kinases play crucial roles in plant stress responses, and research progress on signal perception and transduction is essential. Future challenges include a deeper understanding of the mechanisms of action of protein kinases such as SNRKs, MAPK, CDPKs/CPKs, and RLKs in stress responses.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yaying Xia, Junfeng Yang, Lin Ma, Su Yan, Yongzhen Pang
Summary: The study identified 346 cytochrome P450 genes and found multiple hub genes involved in plant growth and development under drought/salt stresses. These hub genes were induced by the stresses and mainly involved in secondary metabolism and fatty acid pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Xiaoshan Wang, Jing Wang, Juncheng Yin, Junhao Li
Summary: This study aimed to compare the differences in ion accumulation and photosynthetic electron transport between Medicago truncatula and Medicago sativa during NaCl stress, and reveal their different physiological mechanisms of salt resistance. The results showed that M. sativa had lower leaf Na+/K+, Na+/Ca2+, Na+/Mg2+ ratios compared with M. truncatula under NaCl stress, indicating its stronger ability to regulate nutrient ion balance. Moreover, M. sativa exhibited a slower and lower decline in DF amplitude and a later appearance of the lowest points of MR/MRO under NaCl stress, indicating its stronger ability to maintain stable electron transport.
Article
Plant Sciences
Misganaw Wassie, Shurui Song, Liwen Cao, Liang Chen
Summary: This study reveals that overexpression of MtCBL13 in Arabidopsis increases sensitivity to drought stress. Transgenic plants overexpressing MtCBL13 exhibit enhanced sensitivity to mannitol and ABA treatments during seed germination and seedling stages. Furthermore, overexpression of MtCBL13 reduces drought tolerance in Arabidopsis, leading to increased oxidative damage, electrolyte leakage, lipid peroxidation, and water loss. These findings demonstrate that MtCBL13 negatively regulates drought tolerance through an ABA-dependent pathway.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Environmental Sciences
Anna Burger, Marieluise Weidinger, Nils Baumann, Andreas Vesely, Irene Lichtscheidl
Summary: This study investigated the responses of metal-accumulating plants to different concentrations of uranium, finding that roots contained most of the metal and uranium influenced the nutrient profile of the plants. Additionally, the impact of uranium on plant growth and photosynthetic parameters varied depending on the external concentration and plant species.
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
(2021)
Article
Biology
Eva Spitalska, Eva Boldisova, Katarina Palkovicova, Zuzana Sekeyova, Ludovit Skultety
Summary: Ticks can transmit pathogens that can seriously affect the health of animals and humans. Surveillance of rickettsioses and Rickettsia-like infections plays a crucial role in the prevention and management of these communicable diseases.
Article
Plant Sciences
Zahra Ayatollahi, Vaiva Kazanaviciute, Volodymyr Shubchynskyy, Kotryna Kvederaviciute, Manfred Schwanninger, Wilfried Rozhon, Michael Stumpe, Felix Mauch, Sebastian Bartels, Roman Ulm, Salma Balazadeh, Bernd Mueller-Roeber, Irute Meskiene, Alois Schweighofer
Summary: This study reveals the crucial role of different types of MAPK phosphatases in plant stress responses and the tight control of MAPK activities.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Environmental Sciences
Monika Danchenko, Oldrich Benada, L'udovit Skultety, Zuzana Sekeyova
Summary: In this study, a strain of Rickettsia felis was successfully isolated and characterized from a common tick species in Slovakia. It is the first time that R. felis has been isolated from ticks, and further research on its growth properties and gene sequences revealed no nucleotide polymorphisms compared to known strains, but the plasmid pRF delta was absent in this isolate.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Plant Sciences
Pavol Melicher, Petr Dvorak, Yuliya Krasylenko, Alexey Shapiguzov, Jaakko Kangasjarvi, Jozef Samaj, Tomas Takac
Summary: This study characterized the role of FSD1 in response to oxidative stress in Arabidopsis thaliana, and found that its antioxidant function depends on the availability of Cu2+ in the growth media. Chloroplastic localization of FSD1 is crucial for maintaining redox homeostasis.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Liang Zhang, Xinlin Liang, Tomas Takac, George Komis, Xiaojuan Li, Yuan Zhang, Miroslav Ovecka, Yanmei Chen, Jozef Samaj
Summary: In plants, membrane compartmentalization relies on vesicle trafficking for organelle communication. Membrane proteins involved in vesicle trafficking are dynamic and responsive to environmental changes and cellular signals. Therefore, understanding the localization and dynamics of these proteins is crucial for dissecting vesicular trafficking pathways.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Jasim Basheer, Pavol Vadovic, Olga Samajova, Pavol Melicher, George Komis, Pavel Krenek, Michaela Kralova, Tibor Pechan, Miroslav Ovecka, Tomas Takac, Jozef Samaj
Summary: Through TALEN-based knockout of HvMPK3, barley roots gain resistance against Fusarium root rot, possibly by inhibiting hyphal penetration, mitigating ROS production, and enhancing suberin accumulation in the root cell wall.
Article
Chemistry, Physical
Monika Garberova, Ivan Potocnak, Monika Tvrdonova, Martina Bago-Pilatova, Slavka Bekesova, Zuzana Kudlickova, Erika Samoi'ova, Alexandra Kesei'akova, Jan Elecko, Maria Vilkova
Summary: In this study, seven new hydrazones were synthesized and characterized. The biological activities of these compounds were evaluated, and two of them showed excellent anti-proliferative effects in Jurkat cells, indicating their potential as antitumor agents.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Ecology
Yuliya Krasylenko, Karol Rydlo, Natalia Atamas, Yevhen Sosnovsky, Oleksii Horielov, Ivo Macecek, Olga Samajova, Miroslav Ovecka, Tomas Takac, Jozef Samaj
Summary: A novel unmanned aerial vehicle, the Druid Drone (DD), equipped with a manipulator and various functional modules, is used for tree crown management and biodiversity research in forest canopies. It provides integrated pest management by surveying, spraying, and sampling leaves and branches. This method offers a safe, precise, and minimally invasive solution for studying forest canopy biodiversity, tree health care, and mistletoe management.
METHODS IN ECOLOGY AND EVOLUTION
(2023)
Review
Plant Sciences
Pavol Melicher, Petr Dvorak, Jozef Samaj, Tomas Takac
Summary: The regulation of reactive oxygen species (ROS) levels in plants is achieved through various antioxidants and interactions with regulatory and signaling proteins. However, the role of these interactions in antioxidant defense is still not well understood.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Martin Cente, Monika Danchenko, Ludovit Skultety, Peter Filipcik, Zuzana Sekeyova
Summary: This study investigates the signaling pathways involved in the neurotoxic effects of Rickettsia infections in the central nervous system. It demonstrates that Rickettsia infection can cause neuronal damage and neuroinflammatory responses, providing new insights into the pathogenesis of CNS infection.
Article
Immunology
Jozef Kuzma, Sofia Guadalupe Zavala-Meneses, Ludovit Skultety, Dittmar Chmelar, Jozef Ficik, Lenka Palcova
Summary: A total of 78 Clostridium septicum isolates were tested for a-toxin, flagellin genes, and vancomycin resistance. The ability to form a biofilm and antibiotic susceptibility were also assessed. All isolates were positive for a-toxin and flagellin genes, while only 19 isolates showed vancomycin resistance. Strong biofilm formation was observed in isolates from patients with oncologic or septic and febrile diagnoses. There was a correlation between antibiotic resistance and biofilm formation.
Article
Plant Sciences
Olha Lakhneko, Oleg Stasik, Ludovit Skultety, Dmytro Kiriziy, Oksana Sokolovska-Sergiienko, Mariia Kovalenko, Maksym Danchenko
Summary: Drought is a major limitation for sustainable agricultural production, particularly affecting the quality and quantity of bread wheat yield during flowering. Through studying contrasting wheat cultivars, it was found that the sensitive cultivar showed ineffective water management and a decline in photosynthesis, while the tolerant cultivar used photorespiration to dissipate excessive light energy and induced superoxide dismutase for a less inhibited photosynthesis. The tolerant cultivar also exhibited a more stable gluten profile and accumulation of specific proteins, indicating its potential as a marker for drought tolerance in breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Oncology
Petra Chalova, Anton Tazky, Ludovit Skultety, Lenka Minichova, Michal Chovanec, Sona Ciernikova, Peter Mikus, Juraj Piestansky
Summary: Short-chain fatty acids (SCFAs) are the main flow of carbon from diet, through the microbiome to the host, and are important molecules for regulating intestinal homeostasis and impacting the immune system. Analyzing SCFAs using advanced analytical approaches could provide potential diagnostic value for various diseases, including cancer. This review highlights the importance of analyzing SCFAs from clinical and analytical perspective and discusses current advances in sample pretreatment, separation strategy, and detection methods, as well as the potential development of future diagnostic tools based on targeted metabolite profiling in oncology and other diseases.
FRONTIERS IN ONCOLOGY
(2023)