期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 65, 期 4, 页码 1111-1123出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ert463
关键词
Albino; Arabidopsis thaliana; chloroplast development; proplastid to chloroplast transition; slow greening; tetratricopeptide repeat-containing protein
资金
- Ministry of Agriculture of the People's Republic of China [2013ZX08009-003-002]
- National Natural Science Foundation of China (NSFC) [31070247, 91017002, 31271460]
- Open Fund of the National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, CAS
A new gene, SG1, was identified in a slow-greening mutant (sg1) isolated from an ethylmethanesulphonate-mutagenized population of Arabidopsis thaliana. The newly formed leaves of sg1 were initially albino, but gradually became pale green. After 3 weeks, the leaves of the mutant were as green as those of the wild-type plants. Transmission electron microscopic observations revealed that the mutant displayed delayed proplastid to chloroplast transition. The results of map-based cloning showed that SG1 encodes a chloroplast-localized tetratricopeptide repeat-containing protein. Quantitative real-time reverse transcriptionPCR data demonstrated the presence of SG1 gene expression in all tissues, particularly young green tissues. The sg1 mutation disrupted the expression levels of several genes associated with chloroplast development, photosynthesis, and chlorophyll biosynthesis. The results of genetic analysis indicated that gun1 and gun4 partially restored the expression patterns of the previously detected chloroplast-associated genes, thereby ameliorating the slow-greening phenotype of sg1. Taken together, the results suggest that the newly identified protein, SG1, is required for chloroplast development in Arabidopsis.
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