期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 64, 期 3, 页码 807-813出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ers286
关键词
C-4 photosynthesis; cell specificity; mesophyll cells; monocots; RNA isolation
资金
- Bill and Melinda Gates Foundation
In the leaves of most C-4 plants, mesophyll (M) and bundle sheath (BS) cells develop and maintain highly differentiated biochemical networks. Separation and analysis of M and BS cells has greatly influenced our understanding of the C-4 pathway. A number of approaches including mechanical separation, digestion with cell wall-degrading cocktails, laser-capture microdissection, and leaf rolling have been used to isolate these cell types. Although leaf rolling is conceptually and practically the simplest method, to date it has only been used to assess the metabolite content of M cells from C-4 leaves of maize. This study reports an adapted leaf-rolling method for the isolation of high-quality RNA from M cells of sorghum. Analysis of leaf cell structure, RNA integrity, and transcript abundance of marker genes demonstrated that the sap collected by leaf rolling was from M cells and had no significant contamination. It was concluded that leaf rolling is a fast, cheap, and efficient method of measuring transcript abundance in M cells of sorghum.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据