期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 63, 期 15, 页码 5607-5612出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ers211
关键词
Carotenogenic genes; complementation; diadinoxanthin; fucoxanthin; genetic pathway; neoxanthin; Phaeodactylum tricornutum
资金
- EU FP7 GIAVAP project [266408]
The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading to -carotene, putative genes were selected from the genomic database and the function of several of them identified by genetic pathway complementation in Escherichia coli. They included genes encoding a phytoene synthase, a phytoene desaturase, a ?-carotene desaturase, and a lycopene -cyclase. Intermediates of the pathway beyond -carotene, present in trace amounts, were separated by TLC and identified as violaxanthin and neoxanthin in the enriched fraction. Neoxanthin is a branching point for the synthesis of both diadinoxanthin and fucoxanthin and the mechanisms for their formation were proposed. A single isomerization of one of the allenic double bounds in neoxanthin yields diadinoxanhin. Two reactions, hydroxylation at C8 in combination with a keto-enol tautomerization and acetylation of the 3-HO group results in the formation of fucoxanthin.
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