Article
Plant Sciences
Marco Marconi, Krzysztof Wabnik
Summary: Plant development involves complex processes that depend on cell polarity, which regulates anisotropic growth and polar localization of membrane proteins. Cell polarity is critical for various plant developmental processes, including embryogenesis, cell division, and response to external stimuli. The establishment of cell polarity is still not fully understood, and computer models have been proposed and tested to explore the molecular and cellular mechanisms involved. This review provides a comprehensive overview of current understanding and research on computer models of cell polarity in plants, focusing on the proteins and mechanisms involved.
Article
Plant Sciences
Yazhou Duan, Limin Wang, Xueling Li, Wanlei Wang, Jing Wang, Xiaoyu Liu, Yangyang Zhong, Nana Cao, Mengjuan Tong, Weina Ge, Yi Guo, Rui Li
Summary: SKS11 and SKS12 are crucial for regulating the integrity, growth, and guidance of pollen tubes, possibly by modulating ROS levels and the deposition or remodeling of cell wall polysaccharides.
Review
Plant Sciences
Maria Breygina, Ekaterina Klimenko, Olga Schekaleva
Summary: This article focuses on the physiological studies of conifer pollen, describing the main properties and diversity of pollen grains in gymnosperms, as well as their pollination strategies.
Review
Ecology
Jeanne Tonnabel, Patrice David, Tim Janicke, Arnaud Lehner, Jean-Claude Mollet, John R. Pannell, Mathilde Dufay
Summary: The study discusses how sexual selection affects traits involved in pollen-pistil interactions, including Fisherian runaway and good-genes models. The authors review aspects of the molecular and cellular biology of pollen-pistil interactions where sexual selection could potentially operate and point out areas for future research.
TRENDS IN ECOLOGY & EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Cuong Nguyen Huu, Sylvia Plaschil, Axel Himmelbach, Christian Kappel, Michael Lenhard
Summary: Most flowering plants have both male and female reproductive organs, with a widespread adaptation to limit self-fertilization known as self-incompatibility (SI). In some flowering plants, different mating types are associated with different floral morphologies. In the heterostylous plant Primula, a specific gene controls style length and determines the mating types. Furthermore, this gene also determines the female incompatibility type. The findings suggest that brassinosteroid (BR) levels in the stigma and style play a role in controlling pollen performance and prevent self-fertilization.
Article
Plant Sciences
Lian Zhou, Zuzana Vejlupkova, Cedar Warman, John E. Fowler
Summary: Members of the LARP family contain a conserved La module associated with RNA-binding activity. Analysis of the maize gene ZmLARP6c1, specific to pollen, suggests its role in pollen-specific RNA regulation. Mutant plants with an inserted Ds-GFP element in the ZmLARP6c1 gene displayed reduced transmission as males, but not as females, indicating an important regulatory function during male gametophyte development.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Ana Marta Pereira, Diana Moreira, Silvia Coimbra, Simona Masiero
Summary: Angiosperm reproduction depends on the precise growth of the pollen tube through different pistil tissues to achieve double fertilization. A network of communication and signaling cascades mediates interactions between the pollen tube and female tissues, with the transmitting tract tissue playing a vital role in supporting pollen tube growth. Insights into these processes are limited due to difficulties in accessing and manipulating female sporophytic tissues in the pistil.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mengqi Lu, Junqin Zhou, Sisi Jiang, Yanling Zeng, Chang Li, Xiaofeng Tan
Summary: This study reveals the significant roles of fasciclin-like arabinogalactan proteins (FLAs) in pollen tube growth in Camellia oleifera and their potential involvement in ABA signaling regulation.
Article
Plant Sciences
Natalia Serrano, Premysl Pejchar, Hana Soukupova, Martin Hubalek, Martin Potocky
Summary: This study reveals the changes in whole-cell glycerolipid composition during tobacco pollen germination and growth, confirming the important role of phosphatidic acid in the establishment and maintenance of cellular polarity in pollen tubes, and suggesting the involvement of plasma membrane lysophospholipids in pollen germination.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Maria Breygina, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina
Summary: Pollen grains are unique haploid organisms with special composition and structure. Angiosperms and gymnosperms share similar mechanisms for pollen germination but gymnosperms exhibit slower growth rates and reduced reliance on female tissues. The properties of pollen lipids, especially very-long-chain FAs, contribute to these features. By analyzing the absolute content and FA composition of pollen lipids, significant differences were found between species, with spruce pollen exhibiting a higher proportion of saturated and monoene FAs. Lily and tobacco also showed variations in the FA composition between pollen coat and gametophyte cells, with a lower unsaturation index in the coat. Moreover, the absolute content of lipids was highest in lily pollen. This study provides new insights into the FA changes during pollen germination in gymnosperms and angiosperms, and highlights the impact of H2O2 on spruce pollen germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bin Zhang, Chi Zhang, Congge Liu, Aigen Fu, Sheng Luan
Summary: A Golgi-localized Mn transporter named PML3 is essential for rapid cell elongation in young tissues like the pollen tube, and its loss-of-function mutation results in reduced seed yield due to decreased male fertility. The lack of pectin deposition in the pollen tube may cause bursting and slow elongation, highlighting the importance of PML3 in Mn homeostasis for metabolic and trafficking processes required for pectin deposition in rapidly elongating cells.
PLANT COMMUNICATIONS
(2021)
Article
Cell Biology
Daniel S. C. Damineli, Maria Teresa Portes, Jose A. Feijo
Summary: This review article discusses recent advances in understanding ultradian oscillations in plant cells, with a focus on single-cell oscillations. It highlights the importance of quantitative approaches in measuring oscillations and the potential of optogenetics techniques to further research membrane potential oscillations across different cell types.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Iris Aloisi, Chiara Piccini, Giampiero Cai, Stefano Del Duca
Summary: Environmental stresses can negatively impact the performance of pollen, particularly during its development and germination stages. Understanding the molecular mechanisms involved in stress perception and response is crucial for the successful reproduction of plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Kazuki Motomura, Naoya Sugi, Atsushi Takeda, Shohei Yamaoka, Daisuke Maruyama
Summary: The nucleus of the vegetative cell in pollen tubes plays a key role in their directional growth and fertilization. However, mutant plants with immotile vegetative and sperm nuclei show female-targeting behavior in their pollen tubes. Our study further explores the mechanism by which pollen tubes can grow without transcription from the vegetative nucleus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Mengqi Lu, Junqin Zhou, Yiyao Liu, Jin Yang, Xiaofeng Tan
Summary: Salicylic acid (SA) plays a crucial role in pollen tube growth in Camellia oleifera, with exogenous SA and methyl salicylate (MeSA) promoting pollen germination and tube elongation. The regulatory genes NPR1, CoNPR3.1, CoNPR3.2, and CoNPR5, located in the nucleus, show consistent expression patterns in pollen tubes and potentially play important roles in the growth process. This study provides insights into the involvement of SA and MeSA in pollen tube development and the role of NPR genes in this process.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Patricia Domingos, Pedro N. Dias, Barbara Tavares, Maria Teresa Portes, Michael M. Wudick, Kai R. Konrad, Matthew Gilliham, Ana Bicho, Jose A. Feijo
Article
Developmental Biology
Aloysius Wong, Lara Donaldson, Maria Teresa Portes, Jorg Eppinger, Jose A. Feijo, Christoph Gehring
Article
Multidisciplinary Sciences
Robert D. Hoffmann, Maria Teresa Portes, Lene Irene Olsen, Daniel Santa Cruz Damineli, Maki Hayashi, Custodio O. Nunes, Jesper T. Pedersen, Pedro T. Lima, Claudia Campos, Jose A. Feijo, Michael Palmgren
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Shanti Pal Gangwar, Marriah N. Green, Erwan Michard, Alexander A. Simon, Jose A. Feijo, Alexander Sobolevsky
Summary: The structures of Arabidopsis thaliana GLR3.2 LBD show similarities to iGluRs, but with differences in certain secondary structure elements. Mutation R133A significantly increases channel constitutive activity, suggesting that mutating the critical residue for agonist binding leads to a more stable closed clamshell conformation.
Article
Multidisciplinary Sciences
Wangshu Mou, Yun-Ting Kao, Erwan Michard, Alexander A. Simon, Dongdong Li, Michael M. Wudick, Michael A. Lizzio, Jose A. Feijo, Caren Chang
NATURE COMMUNICATIONS
(2020)
Article
Plant Sciences
Kunkun Li, Juan Prada, Daniel S. C. Damineli, Anja Liese, Tina Romeis, Thomas Dandekar, Jose A. Feijo, Rainer Hedrich, Kai Robert Konrad
Summary: The study reveals a close interrelation between Ca2+ and pH signaling in plant cells, with pH playing a crucial role in regulating plant growth and stomatal movement.
Article
Cell Biology
Marcela Hernandez-Coronado, Poliana Coqueiro Dias Araujo, Pui-Leng Ip, Custodio O. Nunes, Ramin Rahni, Michael M. Wudick, Michael A. Lizzio, Jose A. Feijo, Kenneth D. Birnbaum
Summary: This study reveals that plant GLRs play a role in both defense responses and regeneration through dynamic changes in chromatin and transcription. Inhibition of GLR activity increases regeneration efficiency in multiple organ repair systems. This finding provides insights into the trade-off between defense and regeneration in plants.
DEVELOPMENTAL CELL
(2022)
Article
Cell Biology
Daniel S. C. Damineli, Maria Teresa Portes, Jose A. Feijo
Summary: This review article discusses recent advances in understanding ultradian oscillations in plant cells, with a focus on single-cell oscillations. It highlights the importance of quantitative approaches in measuring oscillations and the potential of optogenetics techniques to further research membrane potential oscillations across different cell types.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Custodio de Oliveira Nunes, Jose A. Feijo
Summary: Bioelectricity has been studied extensively in all branches of life since the 18th century. It plays a crucial role in cell growth, proliferation, and behavior, resulting in significant tissue rearrangements during embryology and somatic development. In this study, the focus is on the pollen tube as a biological model, which has unique properties such as rapid apical growth and sensitivity to external cues. The study reviews the latest progress in understanding apical growth from the perspective of bioelectricity-driven morphogenesis.
Meeting Abstract
Biophysics
Carlos Navarro, Holger Gohlke, Jose A. Feijo, Erwan Michard
BIOPHYSICAL JOURNAL
(2022)
Article
Biology
Maria Teresa Portes, Daniel S. C. Damineli, Jose A. Feijo
Summary: Ion-specific probes and fluorescent indicators play key roles in studying ion signaling in plant development, particularly calcium, protons, and anions. The genetically encoded pH-sensitive pHluorin reporter allows for cytosolic measurements in Arabidopsis thaliana pollen tubes, providing actual pH values through calibration. This technique helps identify critical phenotypes and establish the importance of tip-focused pH gradient for pollen tube growth, with potential applications in other experimental systems.
Article
Mycology
Clara Valero, Ana Cristina Colabardini, Patricia Alves de Castro, Lilian Pereira Silva, Laure Nicolas Annick Ries, Lakhansing Pardeshi, Fang Wang, Marina Campos Rocha, Iran Malavazi, Roberto Nascimento Silva, Celso Martins, Patricia Domingos, Cristina Pereira-Silva, Michael J. Bromley, Koon Ho Wong, Gustavo H. Goldman
Summary: This study reveals the key role of the transcription factor ZnfA in Aspergillus fumigatus responses to calcium and echinocandin Caspofungin (CSP), highlighting its involvement in iron homeostasis and cell wall organization. ZnfA works collaboratively with the CrzA transcription factor to modulate stress responses in A. fumigatus, bridging calcium signaling and CSP cellular response and uncovering complex connections among different pathways governing stress sensing and signaling in the fungus.
FRONTIERS IN FUNGAL BIOLOGY
(2021)
Article
Biology
Maria Teresa Portes, Jose A. Feijo
Summary: The ion-selective vibrating probe has been crucial in studying ion dynamics and cell physiology processes in pollen tubes, particularly in ion transport and regulation. Optimization of the vibrating probe technology in Arabidopsis pollen tubes enables quantitative analysis of ion flux, providing valuable insights into cellular and molecular mechanisms.
Meeting Abstract
Biophysics
Jose A. Feijo
BIOPHYSICAL JOURNAL
(2020)