期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 63, 期 1, 页码 355-363出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/err283
关键词
CAX3; deficiency; luciferase; magnesium
资金
- Japanese Society for the Promotion of Science
- Ministry of Agriculture, Forestry, and Fisheries of Japan [IPG-0005]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [21228002] Funding Source: KAKEN
The direct determination of elemental concentrations in plants is laborious. To overcome this, a novel monitoring system for magnesium (Mg) in plants was established. Mg deficiency-induced genes were identified by microarray analysis and transgenic lines that expressed luciferase (LUC) under the control of the Mg deficiency-inducible CAX3 promoter were established. The transgenic lines showed a clear response under low Mg conditions, and the degree of luminescence reflected the accumulation of endogenous CAX3 mRNA. The CAX3 expression pattern was also examined in a previously characterized low Mg-sensitive mutant, mrs2-7. In mrs2-7 mutant plants, CAX3 expression was more than three times higher than in the wild-type. In addition, CAX3 expression was negatively correlated with the shoot Mg concentration. Together, these results indicate that CAX3 transcription is a quantitative marker of the Mg status in Arabidopsis.
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