Article
Agronomy
Mufid Alam, Xuan Tan, Hao Zhang, Guangming Lou, Hanyuan Yang, Yin Zhou, Amjad Hussain, Parashuram Bhantana, Gonghao Jiang, Yuqing He
Summary: This study analyzed QTLs related to protein content in two introgression line populations of rice. A total of 25 QTLs were identified, with 19 of them co-locating with previously reported QTLs. Among them, QTL qGPC7.3 was validated as a gene related to total protein content in near-isogenic lines. This research lays the groundwork for further cloning of genes associated with nutritional quality in rice grains.
Article
Agronomy
Mufid Alam, Yingying Wang, Jianxian Chen, Guangming Lou, Hanyuan Yang, Yin Zhou, Saurav Luitel, Gonghao Jiang, Yuqing He
Summary: This study investigated the genetic factors influencing grain protein fractions and content in rice grain. Multiple QTLs related to albumin and grain protein content were identified and validated. The findings provide valuable insights into the functional genes associated with rice grain protein content and the development of nutritionally important rice cultivars using marker-assisted selection breeding.
MOLECULAR BREEDING
(2023)
Article
Chemistry, Applied
Nitya Sharma, Jatindra K. Sahu, Ankur Choudhary, Maninder Meenu, Vasudha Bansal
Summary: Foxtail millet protein and its fractions were efficiently modulated using high-intensity ultrasound, resulting in significant changes in their structural, physicochemical, and functional properties. This study demonstrates the potential of functionalizing foxtail millet protein to enhance its utilization as a plant protein source.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Xiaoyan Tan, Changfeng Li, Yeming Bai, Robert G. Gilbert
Summary: This study investigates the effects of protein fractions with different digestion properties on starch digestibility in chickpea seed. The results show that the major insoluble proteins affecting starch hydrolysis are the glutelin-like and globulin-like fractions. The content of the globulin-like fraction is negatively correlated with starch digestibility.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Ulfah Najamuddin, Sara Ghorbani Gorji, Melissa Fitzgerald
Summary: Protein is an essential macronutrient, but current sources may not meet future demand. Rice bran protein shows potential as an alternative source of protein. This study identified genetic variability in rice bran protein and optimized protein extraction methods.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Agronomy
Lu Gan, Baosheng Huang, Zhaojian Song, Yachun Zhang, Yujie Zhang, Si Chen, Liqi Tong, Zhisong Wei, Lingxiang Yu, Xiangbo Luo, Xianhua Zhang, Detian Cai, Yuchi He
Summary: The study revealed that polyploidization significantly increased the protein content of rice seeds, particularly glutelin, by influencing gene expression, protein synthesis, and storage processes. Variations in glutelin accumulation between tetraploid and diploid rice were mainly manifested in the timing, duration, and levels of gene expressions during seed filling stages.
Article
Biochemistry & Molecular Biology
Deepanwita Chandra, Kyoungwon Cho, Hue Anh Pham, Jong-Yeol Lee, Oksoo Han
Summary: In this study, the expression of rice glutelins was suppressed using CRISPR-Cas9 gene-editing technology to generate transgenic rice variant lines. These variant lines showed altered starch and protein content, reduced grain weight, and irregularly shaped protein aggregates in mature seeds. Transcriptional profiling revealed significant changes in genes associated with RNA processing and protein translation, suggesting that rice glutelin genes may interact with genes involved in starch and seed storage protein synthesis through post-transcriptional and translational mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Yuchen Zhang, Rachel Komorek, Jiyoung Son, Shawn Riechers, Zihua Zhu, Janet Jansson, Christer Jansson, Xiao-Ying Yu
Summary: PGPR play a crucial role in biological control and pathogenic defense on and within plant tissues, and ToF-SIMS imaging reveals that different PGPR have distinct effects on host seed surface, leading to changes in chemical composition and morphology. Metabolite products and biomarkers show different distributions on the seed surface for the two PGPR strains, indicating unique responses from the host.
Article
Biochemistry & Molecular Biology
Yihao Yang, Ziyan Shen, Youguang Li, Chenda Xu, Han Xia, Hao Zhuang, Shengyuan Sun, Min Guo, Changjie Yan
Summary: This article presents a method to improve rice cooking and eating quality by using CRISPR/Cas9 technology to knock out genes that encode grain storage proteins.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Review
Plant Sciences
Liyang Su, Siqi Wan, Junmei Zhou, Qing Song Shao, Bingcong Xing
Summary: This review discusses the roles of transcription factors (TFs) in regulating plant seed development, noting that different TFs or TF families may have similar functions in this process, and some members of the same TF family may have opposite roles.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Joo Sun Lee, Chan Seop Ko, Yong Weon Seo
Summary: The importance of oats has increased due to their high nutritional value and health benefits in humans, but there have been no reports or studies on oat DA1 genes. In this study, three DA1-like genes were identified in oats, and AsDA1-2D was found to be responsible for high-temperature stress tolerance. AsDA1-2D was shown to physically interact with oat-storage globulin and a protease inhibitor, and it negatively regulated oat-grain-storage-globulin under heat stress.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Miriam Marin-Sanz, Francisco Barro
Summary: Silencing gliadins in wheat can reduce the immunogenic response but may lead to an increase in other grain proteins. Transcriptomic analysis revealed a network of transcription factors regulating seed storage protein synthesis and identified differentially expressed genes in the leaf related to nutrient availability and transport.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Shivaranjani Vutharadhi, Siva Kumar Nadimpalli
Summary: Momordica charantia seeds contain a well-characterized galactose specific lectin and various glycosidases. This study successfully purified the lectin and isolated the β-hexosaminidase from the seed extracts. The β-hexosaminidase was further analyzed for molecular properties, immunological cross-reactivity, and sequence identity.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agronomy
Natalia Carolina Moraes Ehrhardt-Brocardo, Cileide Maria Medeiros Coelho, Clovis Arruda Souza
Summary: This study characterized the seed storage protein composition of common bean landrace genotypes during germination and verified the association between seed protein composition and seed vigor. The results showed that protein content and the presence of specific proteins, such as lipoxygenase, were closely related to seed vigor.
ACTA SCIENTIARUM-AGRONOMY
(2022)
Article
Plant Sciences
Yanping Wang, Jinfang Wang, Shaogui Guo, Shouwei Tian, Jie Zhang, Yi Ren, Maoying Li, Guoyi Gong, Haiying Zhang, Yong Xu
Summary: The study demonstrates that ClBG1 may play a crucial role in regulating watermelon seed size and germination primarily through modulation of ABA content and transcriptional regulation of cytoskeleton-, cell cycle-, and signaling-related genes.
HORTICULTURE RESEARCH
(2021)
Review
Food Science & Technology
Olivier Tranquet, Colette Larre, Sandra Denery-Papini
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2020)
Review
Food Science & Technology
Rosa Pilolli, Chiara Nitride, Nathalie Gillard, Anne-Catherine Huet, Christof van Poucke, Marc de Loose, Olivier Tranquet, Collette Larre, Karine Adel-Patient, Herve Bernard, E. N. Clare Mills, Linda Monaci
FOOD RESEARCH INTERNATIONAL
(2020)
Article
Biochemistry & Molecular Biology
Mehdi Cherkaoui, Virginie Lollier, Audrey Geairon, Axelle Bouder, Colette Larre, Helene Rogniaux, Elisabeth Jamet, Fabienne Guillon, Mathilde Francin-Allami
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Agriculture, Multidisciplinary
Clelia Villemin, Olivier Tranquet, Veronique Sole-Jamault, Joost J. Smit, Raymond H. H. Pieters, Sandra Denery-Papini, Gregory Bouchaud
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Review
Food Science & Technology
Kamel-Eddine El Mecherfi, Svetoslav Dimitrov Todorov, Marcela Albuquerque Cavalcanti de Albuquerque, Sandra Denery-Papini, Roberta Lupi, Thomas Haertle, Bernadette Dora Gombossy de Melo Franco, Colette Larre
Article
Allergy
Joana Costa, Simona Lucia Bavaro, Sara Benede, Araceli Diaz-Perales, Cristina Bueno-Diaz, Eva Gelencser, Julia Klueber, Colette Larre, Daniel Lozano-Ojalvo, Roberta Lupi, Isabel Mafra, Gabriel Mazzucchelli, Elena Molina, Linda Monaci, Laura Martin-Pedraza, Cristian Piras, Pedro M. Rodrigues, Paola Roncada, Denise Schrama, Tanja Cirkovic-Velickovic, Kitty Verhoeckx, Caterina Villa, Annette Kuehn, Karin Hoffmann-Sommergruber, Thomas Holzhauser
Summary: This research searched for evidence on how different physicochemical properties affect the allergenicity of food proteins, and found consistent effects on protein allergenicity within the same protein family. Molecular stability was identified as the most common characteristic promoting plant protein allergenicity. However, there is still a lack of systematic approach to link physicochemical properties with clinical allergenicity.
CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY
(2022)
Article
Allergy
Joana Costa, Caterina Villa, Kitty Verhoeckx, Tanja Cirkovic-Velickovic, Denise Schrama, Paola Roncada, Pedro M. Rodrigues, Cristian Piras, Laura Martin-Pedraza, Linda Monaci, Elena Molina, Gabriel Mazzucchelli, Isabel Mafra, Roberta Lupi, Daniel Lozano-Ojalvo, Colette Larre, Julia Klueber, Eva Gelencser, Cristina Bueno-Diaz, Araceli Diaz-Perales, Sara Benede, Simona Lucia Bavaro, Annette Kuehn, Karim Hoffmann-Sommergruber, Thomas Holzhauser
Summary: Physicochemical parameters play a crucial role in shaping the allergenicity of animal proteins. However, there are significant knowledge gaps in understanding protein allergenicity from both animal and plant origins, necessitating further comprehensive research and data integration in the future.
CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY
(2022)
Editorial Material
Nutrition & Dietetics
Katharina Anne Scherf, Carlo Catassi, Fernando G. Chirdo, Paul J. Ciclitira, Conleth Francis Feighery, Carmen Gianfrani, Frits Koning, Knut E. A. Lundin, Stefania Masci, Detlef Schuppan, Marinus J. M. Smulders, Olivier Tranquet, Riccardo Troncone, Peter Koehler
FRONTIERS IN NUTRITION
(2021)
Article
Chemistry, Applied
Rosa Pilolli, Christof Van Poucke, Elisabetta De Angelis, Chiara Nitride, Marc de Loose, Nathalie Gillard, Anne-Catherine Huet, Olivier Tranquet, Colette Larre, Karine Adel-Patient, Herve Bernard, E. N. Clare Mills, Linda Monaci
Summary: The study focuses on discovering the most reliable peptide markers in multiple food matrices, laying the foundation for the development of a mass spectrometry method for multiple allergen detection.
Article
Biotechnology & Applied Microbiology
Kamel-Eddine El Mecherfi, Roberta Lupi, Mehdi Cherkaoui, Marcela A. C. Albuquerque, Svetoslav Dimitrov Todorov, Olivier Tranquet, Caroline Klingebiel, Helene Rogniaux, Sandra Denery-Papini, Bernard Onno, Bernadette Dora Gombossy de Melo Franco, Colette Larre
Summary: The study found that the fermentation by Lc. lactis LLGKC18 can degrade the main gluten allergenic proteins. Proteomic analysis showed a significant decrease in the antigenicity and allergenicity of gluten after fermentation by Lc. lactis LLGKC18, indicating its potential for reducing wheat allergy.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Review
Nutrition & Dietetics
Sabrina Geisslitz, Peter Shewry, Fred Brouns, Antoine H. P. America, Giacomo Pietro Ismaele Caio, Matthew Daly, Stefano D'Amico, Roberto De Giorgio, Luud Gilissen, Heinrich Grausgruber, Xin Huang, Daisy Jonkers, Daniel Keszthelyi, Colette Larre, Stefania Masci, Clare Mills, Marie Sofie Moller, Mark E. Sorrells, Birte Svensson, Victor F. Zevallos, Peter Louis Weegels
Summary: Amylase/trypsin-inhibitors (ATIs) are proteins in wheat grains that may play a role in natural defense and adverse reactions in humans. Studies have shown that ATIs can activate the innate immune system, potentially affecting conditions like coeliac disease and non-coeliac wheat sensitivity. Further research is needed to understand the mechanisms and implications of ATIs in wheat.
FRONTIERS IN NUTRITION
(2021)
Article
Biotechnology & Applied Microbiology
Jean-Lou Reyre, Sacha Grisel, Mireille Haon, David Navarro, David Ropartz, Sophie Le Gall, Eric Record, Giuliano Sciara, Olivier Tranquet, Jean-Guy Berrin, Bastien Bissaro
Summary: Filamentous fungi, such as Ustilago maydis, play a key role in regulating various processes on Earth. They secrete carbohydrate-active enzymes (CAZymes) to modify and degrade carbohydrates. In this study, researchers analyzed the secretome of U. maydis and identified several CAZymes involved in the modification of the fungal cell wall components. They characterized two new CAZymes and found a potential biocatalytic cascade. These findings provide insights into the role of CAZymes in fungi and highlight the need for further research in this area.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Nutrition & Dietetics
Katharina A. Scherf, Carlo Catassi, Fernando Chirdo, Paul J. Ciclitira, Conleth Feighery, Carmen Gianfrani, Frits Koning, Knut E. A. Lundin, Detlef Schuppan, Marinus J. M. Smulders, Olivier Tranquet, Riccardo Troncone, Peter Koehler
FRONTIERS IN NUTRITION
(2020)