Article
Plant Sciences
Hong Zhao, Yanzhai Song, Junhui Li, Yue Zhang, Huaqiu Huang, Qun Li, Yu'e Zhang, Yongbiao Xue
Summary: In this study, the researchers investigated the ubiquitination patterns of Petunia hybrida S-3-RNase and its effects. They found that R II is the major ubiquitination region, removal of which significantly reduces seed sets from cross-pollination. This research reveals the stepwise UPS mechanism that restricts the cytotoxicity of S-RNase in P. hybrida for successful cross-pollination.
Article
Forestry
Daouda Coulibaly, Guofeng Hu, Zhaojun Ni, Kenneth Omondi Ouma, Xiao Huang, Shahid Iqbal, Chengdong Ma, Ting Shi, Faisal Hayat, Benjamin Karikari, Zhihong Gao
Summary: This study identified SFB genotypes of Japanese apricot using AS-PCR amplification method and sequencing, and isolated 11 novel SFB alleles that are uniquely expressed in pollen. These results provide a valuable resource for studying pollen self-incompatibility mechanisms.
Article
Plant Sciences
Koji Kawamura, Yoshihiro Ueda, Shogo Matsumoto, Takanori Horibe, Shungo Otagaki, Li Wang, Guoliang Wang, Laurence Hibrand-Saint Oyant, Fabrice Foucher, Marcus Linde, Thomas Debener
Summary: This study identified the S-locus controlling self-incompatibility in Rosa, and confirmed the genetic linkage between the newly-identified S-RNase S-C2 and the previously-identified S-C1 in chromosome 3. Through breeding history and wild species analysis, it was found that different S-alleles are present in continuous-flowering cultivars, and S-genotyping is helpful for effective breeding.
HORTICULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Muhammad Husnain Ahmad, Xin Zheng, Yu Hu, Huimin Liu, Yi Sun, Hao Wen, Lijun Chai
Summary: In this study, a rapid method using agarose gel electrophoresis and aniline blue staining was developed to determine the pollen compatibility of citrus cultivars and observe the growth status of pollen tubes. This method is simple and cost-effective, and it helps in selecting suitable pollinizer trees and parents for breeding programs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Plant Sciences
Chitose Honsho, Koichiro Ushijima, Misa Anraku, Shuji Ishimura, Qibin Yu, Frederick G. Gmitter, Takuya Tetsumura
Summary: This study identified genes regulating self-incompatibility in Japanese citrus cultivars through RNA-seq, revealing that six T2 RNase genes were S-RNases. The findings support the notion that citrus self-incompatibility is based on S-RNase. Further homology comparisons with S-RNases from Chinese citrus led to the conclusion that three of the T2 RNases were novel citrus S-RNases involved in self-incompatibility.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Agnes Kivistik, Liina Jakobson, Kersti Kahu, Kristiina Laanemets
Summary: In this study, it was found that a significant proportion of sweet cherry cultivars in Estonia, including Estonian varieties, carry the rare S-allele S-17, with some exhibiting self-compatibility. This indicates potential heritable reduced effectiveness of self-infertility among these cultivars. The newly genotyped sweet cherry cultivars were classified into existing and proposed incompatibility groups, with those carrying S-17 suggested as excellent pollinators across Europe.
PLANT MOLECULAR BIOLOGY REPORTER
(2022)
Article
Genetics & Heredity
Amanda K. Broz, Christopher M. Miller, You Soon Baek, Alejandro Tovar-Mendez, Pablo Geovanny Acosta-Quezada, Tanya Elizabet Riofrio-Cuenca, Douglas B. Rusch, Patricia A. Bedinger
Summary: This study investigated the mechanisms leading to loss of S-RNase function in the Solanaceae family, identifying mutations and transcriptionally silenced genes. The research enriches our understanding of the self-incompatibility system in plants.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Sarah Lee, Felix E. Enciso-Rodriguez, William Behling, Thilani Jayakody, Kaela Panicucci, Daniel Zarka, Satya Swathi Nadakuduti, C. Robin Buell, Norma C. Manrique-Carpintero, David S. Douches
Summary: Using CRISPR-Cas9 gene editing, the S-RNase and HT-B genes were knocked out in diploid self-incompatible potato plants, resulting in self-compatible lines. The study also revealed a synergistic effect between HT-B and S-RNase in self-compatibility. The germplasm generated from this study will be valuable for potato breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Dipnarayan Saha, Subhojit Datta, Arun Kumar Shaw, Maruthi Rangappa Thimmaiah, Pratik Satya, Jiban Mitra, Gouranga Kar
Summary: This study generated a high-quality de novo transcriptome to investigate the LSI in C. juncea and identified a candidate gene, Cjun_RNS3.1, that may be involved in preventing self-fertilization in the male gametophyte.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Jianbing Hu, Chenchen Liu, Zezhen Du, Furong Guo, Dan Song, Nan Wang, Zhuangmin Wei, Jingdong Jiang, Zonghong Cao, Chunmei Shi, Siqi Zhang, Chenqiao Zhu, Peng Chen, Robert Larkin, Zongcheng Lin, Qiang Xu, Junli Ye, Xiuxin Deng, Maurice Bosch, Vernonica Franklin-Tong, Lijun Chai
Summary: A new mechanism for self-incompatibility (SI) to self-compatibility (SC) conversion in citrus, which involves the insertion of MITE elements, has been discovered. This study provides the first evidence of the role of MITEs in the S-locus, revealing their importance in the loss of SI phenotype.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Lei Wu, Xing Liu, Ming-Yue Zhang, Kai-Jie Qi, Xue-Ting Jiang, Jia-Long Yao, Shao-Ling Zhang, Chao Gu
Summary: Through transcriptome and real-time PCR analyses, two leucine-rich repeat extensin genes, PbLRXA2.1 and PbLRXA2.2, were identified in pear. These genes were found to be significantly upregulated in pollen grains and pollen tubes of the self-compatible cultivar, 'Jinzhui', compared to the self-incompatible cultivar, 'Yali'. PbLRXA2.1 and PbLRXA2.2 promoted pollen tube growth and attenuated the inhibitory effects of self S-RNase, indicating their involvement in the loss of self-incompatibility. The promoters of these two genes were directly bound by the ABRE-binding factor PbABF.D.2, and knockdown of PbABF.D.2 inhibited pollen tube growth. Additionally, self S-RNase repressed the expression of PbLRXA2.1, PbLRXA2.2, and PbABF.D.2, suggesting its role in arresting pollen tube growth by restricting the PbABF.D.2-PbLRXA2.1/PbLRXA2.2 signal cascade.
Article
Genetics & Heredity
Jie Yu, Baoan Wang, Wenqi Fan, Songbo Fan, Ya Xu, Chunsheng Liu, Tianxing Lv, Wanda Liu, Ling Wu, Linfeng Xian, Tianzhong Li
Summary: The study showed that pollen tube polyamines respond to self-S-RNase and help defend against it, potentially providing a way to break self-incompatibility in apple by enhancing pollen tube tolerance.
Article
Horticulture
Ayda Boubakri, Lamia Krichen, Mohamed-Amine Batnini, Neila Trifi-Farah, Guillaume Roch, Jean-Marc Audergon, Hedia Bourguiba
Summary: The study identified diverse S-alleles within the Tunisian apricot germplasm, allocating the accessions into 14 cross-incompatibility groups, with newly identified self-compatible accessions. This reflects the domestication and diffusion history of apricot species. Understanding the incompatibility relationships among apricot accessions is crucial for future breeding programs and orchard design.
SCIENTIA HORTICULTURAE
(2021)
Article
Biochemistry & Molecular Biology
Zhouwen Wang, Meng Wang, Yi Ding, Tao Li, Senrong Jiang, Shaoling Kang, Shuangshuang Wei, Jun Xie, Jiaquan Huang, Wenbin Hu, Hongli Li, Hua Tang
Summary: Through transcriptome sequencing, we identified differentially expressed genes (DEGs) in the pitaya stigmas under different pollination types, especially at the late post-pollination stage. We also tentatively identified HuS-RNase2 as a candidate gynogenetic S gene in the pitaya GSI system based on the available transcriptome data and bioinformatics analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yu-Ze Li, Jia-Wei Zhu, Wei Lin, Mo-Ying Lan, Cong Luo, Li-Ming Xia, Yi-Li Zhang, Rong-Zhen Liang, Wang-Li Hu, Gui-Xiang Huang, Xin-Hua He
Summary: S-RNase plays a vital role in self-incompatibility in Rutaceae plants. This study identified four S-RNase gene sequences from the 'XiangShui' lemon genome and ubiquitome, and showed that S-3-RNase may play an important role in self-incompatibility in 'XiangShui' lemon through its expression pattern and interactions with other proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)