Article
Physiology
Chen-Chung Steven Liu, Pui Wen Cheung, Anupama Dinesh, Noah Baylor, Theodor C. Paunescu, Anil Nair, Richard Bouley, Dennis Brown
Summary: The Arp2/3 complex plays a crucial role in the trafficking of AQP2, specifically in preventing it from entering the post-trans-Golgi network exocytotic pathway and blocking AQP2 membrane accumulation. This study provides new insights for modulating AQP2 trafficking and treating water balance disorders.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Alexandra Colin, Laurent Blanchoin
Summary: A new study reveals the importance of WASP- and actin-filament-mediated activation of the Arp2/3 complex in the assembly of functional actin networks.
Article
Cell Biology
Sang A. Mun, Jongseo Park, Kyoung Ryoung Park, Youngjin Lee, Jung Youn Kang, Taein Park, Minwoo Jin, Jihyeong Yang, Chang-Duk Jun, Soo Hyun Eom
Summary: Ca2+ regulates cellular functions and EFhd1, a mitochondrial protein, contributes to mitochondrial morphology and energy synthesis by binding β-actin. Structural comparisons show similarities between EFhd1, EFhd2, and AIF-1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Junning Huang, Haiqin Zhou, Caichun Tan, Shien Mo, Tingji Liu, Yan Kuang
Summary: This study aims to investigate the mechanism of Actin Related Protein 2/3 Complex Subunit 1B (ARPC1B) in the progression of ovarian cancer. The overexpression of ARPC1B in ovarian cancer was found to be correlated with poor prognosis. ARPC1B promoted cell proliferation, migration, and invasion through activation of Wnt/b-catenin signaling pathway in ovarian cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Neurosciences
Weimin Ni, Jie Zhang, Bing Wang, Feng Liang, Long Bao, Pengfei Li, Yan Fang
Summary: Obesity is a major health crisis in modern society, and a high-fat diet can induce hypothalamic inflammation and leptin resistance. Recent studies have shown that ARPC1B, an actin-binding protein highly expressed in immune cells, has anti-inflammatory effects. This study aimed to investigate the role of ARPC1B in HFD-induced obesity and found that up-regulation of ARPC1B in the hypothalamic arcuate nucleus can inhibit the development of obesity.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Qiang-qiang Xia, Angela K. Walker, Chenghui Song, Jing Wang, Anju Singh, James A. Mobley, Zhong X. Xuan, Jeffrey D. Singer, Craig M. Powell
Summary: Autism Spectrum Disorder (ASD) is a developmental disorder characterized by repetitive behavior, restricted range of interests, and atypical social interaction and communication. The CUL3 gene, which codes for a scaffold protein involved in assembly of ubiquitin ligase complexes, has been identified as a high-risk gene for autism. In a mouse model, heterozygous deletion of Cul3 leads to reduced CUL3 protein levels and impaired spatial object recognition memory, but does not cause major abnormalities in hippocampal neuronal morphology, function, or social interaction.
Article
Genetics & Heredity
Samar Ijaz Gul, Aqsa Ayoub, Syed Aoun Ali, Sharoon Hanook, Deeba Noreen Baig
Summary: The study revealed significant dysregulation in the transcriptional pattern of Pfn3 in brain tumors, suggesting a potential correlation between molecular variations in Pfn3 and the malignancy of brain tumors, similar to the role of tumor suppressor genes in other types of cancer.
Article
Biochemistry & Molecular Biology
Kristina Tugaeva, Dorothy E. D. P. Hawkins, Jake L. R. Smith, Oliver W. Bayfield, De-Sheng Ker, Andrey A. Sysoev, Oleg Klychnikov, Alfred A. Antson, Nikolai N. Sluchanko
Summary: Phosphorylated SARS-CoV-2 N protein binds to 14-3-3 protein, potentially playing a crucial role in nucleocytoplasmic shuttling and other functions. A critical phosphorylation site located within the SR-rich region of N, which is conserved among related coronaviruses, interacts with all seven human 14-3-3 isoforms.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Oscar M. Camacho, Andrew Hill, Stacy Fiebelkorn, Aaron Williams, James Murphy
Summary: The study suggests that more complex models may be needed to assess interactions between multiple coexisting products in the marketplace. Data availability may hinder the effectiveness of three-product models, while two-product models could miss potential synergies between products. Scenario analyses for THPs and e-cigarettes in Italy show reductions in life-years lost, with larger reductions seen in the Japanese THP scenario compared to the USA e-cigarette projections.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Biology
Pavel Pohl, Rohit Joshi, Olivia Petrvalska, Tomas Obsil, Veronika Obsilova
Summary: The study investigated the structural basis of Nedd4-2 regulation by 14-3-3 and identified phosphorylated Ser342 and Ser448 as key residues facilitating 14-3-3 binding. The Nedd4-2:14-3-3 complex induces a structural rearrangement of Nedd4-2 by inhibiting interactions between its structured domains.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Shelley R. Wang, Navneeta Rathor, Min S. Kwon, Lan Xiao, Hee Kyoung Chung, Douglas J. Turner, Jian-Ying Wang, Jaladanki N. Rao
Summary: miR-195 plays a regulatory role in early intestinal epithelial restitution by altering the expression of ARP-2. Cellular polyamines decrease miR-195 abundance and enhance ARP-2 translation. Overexpression of miR-195 disrupts F-actin cytoskeleton organization, which can be prevented by ARP2 overexpression.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Immunology
Xia Chen, Li Wang, Limin Cao, Tiantian Li, Zhihong Li, Yumeng Sun, Jinqiu Ding, Chun Zhou, Yadong Xie, Nan Yue, Jing Nan, Xin-Ming Jia, Chao Peng, Huabin Li, Jinbo Yang, Hui Xiao
Summary: LRRC8 VRACs are heteromeric hexamers that transport chloride, taurine, glutamate, cisplatin, cGAMP, and cyclic dinucleotides. The presence of LRRC8A/E-containing VRACs in serum allows for their opening under resting conditions through the involvement of a protein component, cGAMP synthase, and phospholipid PIP2. This activation of VRACs by serum and TNF enhances the uptake of cisplatin in cancer cells, suggesting a potential new approach for infection and cancer treatment.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Raquel R. Lopes, Catarina S. Tome, Roberto Russo, Roberta Paterna, Joao Leandro, Nuno R. Candeias, Lidia M. D. Goncalves, Miguel Teixeira, Pedro M. F. Sousa, Rita C. Guedes, Joao B. Vicente, Pedro M. P. Gois, Paula Leandro
Summary: Phenylketonuria (PKU) is a genetic disease caused by deficient activity of human phenylalanine hydroxylase (hPAH) and protein misfolding is recognized as the main underlying pathogenic mechanism of PKU. 3-hydroxyquinolin-2(1H)-one derivatives were found to act as protectors of hPAH enzyme activity, affecting the coordination of the non-heme ferric center at the enzyme active site. These stabilizing compounds were shown to be outcompeted by the natural substrate l-phenylalanine, but two compounds functionally stabilized hPAH by maintaining protein activity.
Review
Immunology
Anton Kamnev, Claire Lacouture, Mathieu Fusaro, Loic Dupre
Summary: This review discusses how the generation and adaptation of diversified motility patterns in immune cells is sustained by actin cytoskeleton dynamics, while also reviewing the knowledge gained through the study of inborn errors of immunity related to actin defects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Peng Gao, Li Qin, Hanh Nguyen, Huajin Sheng, Teagen D. Quilichini, Daoquan Xiang, Leon V. Kochian, Yangdou Wei, Raju Datla
Summary: The actin cytoskeleton plays a critical role in plant defense against pathogens. This study used CRISPR/Cas9 technology to generate Arabidopsis plants with ARP2 knockout, and identified the similar function of ARP2 to ARP3 in the accumulation of PEN1 against fungal invasion.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
M. Srba, A. Cernikova, Z. Opatrny, L. Fischer
BIOLOGIA PLANTARUM
(2016)
Article
Plant Sciences
Milos Duchoslav, Lukas Fischer
Article
Cell Biology
Pavel Marasek, Rastislav Dzijak, Irina Studenyak, Jindriska Fiserova, Livia Ulicna, Petr Novak, Pavel Hozak
JOURNAL OF CELL SCIENCE
(2015)
Article
Biochemistry & Molecular Biology
Lenka Havelkova, Gitanjali Nanda, Jan Martinek, Erica Bellinvia, Lenka Sikorova, Katerina Slajcherova, Daniela Seifertova, Lukas Fischer, Jindriska Fiserova, Jan Petrasek, Katerina Schwarzerova
Article
Cell Biology
Jindriska Fiserova, Michaela Efenberkova, Tomas Sieger, Miloslava Maninova, Jana Uhlirova, Pavel Hozak
JOURNAL OF CELL SCIENCE
(2017)
Article
Plant Sciences
Dimitrij Tyc, Eva Nocarova, Lenka Sikorova, Lukas Fischer
PLANT CELL REPORTS
(2017)
Article
Multidisciplinary Sciences
Dana Hola, Monika Benesova, Lukas Fischer, Daniel Haisel, Frantisek Hnilicka, Helena Hnilickova, Petr L. Jedelsky, Marie Kocova, Dagmar Prochazkova, Olga Rothova, Lenka Tumova, Nada Wilhelmova
Article
Plant Sciences
Vojtech Cermak, Lukas Fischer
Article
Multidisciplinary Sciences
K. Schwarzerova, E. Bellinvia, J. Martinek, L. Sikorova, V Dostal, L. Libusova, P. Bokvaj, L. Fischer, A. C. Schmit, P. Nick
SCIENTIFIC REPORTS
(2019)
Article
Genetics & Heredity
Adela Pribylova, Vojtech Cermak, Dimitrij Tyc, Lukas Fischer
EPIGENETICS & CHROMATIN
(2019)
Article
Multidisciplinary Sciences
Pengwei Wang, Roman Pleskot, Jingze Zang, Joanna Winkler, Jie Wang, Klaas Yperman, Tong Zhang, Kun Wang, Jinli Gong, Yajie Guan, Christine Richardson, Patrick Duckney, Michael Vandorpe, Evelien Mylle, Jindriska Fiserova, Daniel Van Damme, Patrick J. Hussey
NATURE COMMUNICATIONS
(2019)
Article
Plant Sciences
A. Pecinka, P. Schrumpfova, L. Fischer, E. Tomastikova, I Mozgova
BIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Adela Pribylova, Lukas Fischer, Douglas E. Pyott, Andrew Bassett, Attila Molnar
Summary: This study investigates the impact of epigenetic modifications, specifically cytosine methylation, on the efficacy of CRISPR/Cas9-induced mutations in plants. It is found that high levels of promoter methylation can decrease the frequency of Cas9-mediated mutations and influence the ratio of insertions and deletions. The study also uncovers a novel type of DNA break and repair mechanism in plant cells.
Article
Biochemistry & Molecular Biology
Vojtech Cermak, Dimitrij Tyc, Adela Pribylova, Lukas Fischer
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2020)
Article
Biochemistry & Molecular Biology
Jindriska Fiserova, Miloslava Maninova, Tomas Sieger, Jana Uhlirova, Lenka Sebestova, Michaela Efenberkova, Martin Capek, Karel Fiser, Pavel Hozak
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)