Article
Cell Biology
Shiri Avivi Kela, Kriti Sethi, Pei Yi Tan, Danesha Suresh, Hui Ting Ong, Perla G. Castaneda, Mustafi R. Amin, Tal Laviv, Erin J. Cram, Jan Faix, Ronen Zaidel-Bar
Summary: This study identified a novel mechanotransduction pathway in the C. elegans spermatheca, which involves tension-dependent localization of a RhoGEF and a positive feedback loop for RHO-1-MyosinII activation. It provides insights into how epithelial tubes can sense and adjust their contractility in response to internal pressure.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Zizhen Zhou, Jia Zhao, Yanan Liu, Xiaoyu Yan, Hongyu Sun, Meihui Xia, Jing Su
Summary: The study demonstrates a close association between autophagy and invasion in malignant tumors, with autophagy playing a promoting role in the invasiveness of cancer cells.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Oncology
Ji-Won Ryu, Ha-Yeon Shin, Hyo-Sun Kim, Gwan Hee Han, Jeong Won Kim, Hae-Nam Lee, Hanbyoul Cho, Joon-Yong Chung, Jae-Hoon Kim
Summary: Hormonal factors, particularly steroid hormones, are believed to play a significant role in ovarian cancer development. The expression of glucocorticoid receptor (GR) has been associated with poor prognosis in epithelial ovarian cancer. This study investigates the clinical significance of beta-arrestin expression in ovarian cancer and the potential synergistic effect with GR as prognostic factors. The findings demonstrate a moderate correlation between beta-arrestin 1 and GR expression, suggesting a possible role in prognostication.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ling Zhu, Yan-Yu Zhou, Zhi Zhang, Su-Qing Yin, Dong-Dong Lv, Yu-Ling Wu, Bao-Shan Wang, Meng-Han Mao, Ying-Fu Jiao, Wei-Feng Yu, Po Gao, Li-Qun Yang
Summary: Remifentanil enhances liver regeneration by promoting hepatocyte proliferation rather than anti-inflammatory effects. This effect was confirmed both in vitro and in vivo, and the underlying mechanism may involve the beta-arrestin 2/ERK/cyclin D1 axis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xiaoqin Zhang, Wenqian Zhou, Yangyang Niu, Saiya Zhu, Yingying Zhang, Xiaogang Li, Chen Yu
Summary: Lysyl oxidase (LOX) plays an essential role in renal fibrosis by catalyzing collagen over cross-linking. Increased LOX expression leads to deposition and cross-linking of collagen. LOX is regulated by the beta-arrestin-ERK-STAT3 pathway. Inhibition of LOX improves renal fibrosis.
Article
Biotechnology & Applied Microbiology
Feng Cao, Cheng Huang, Jiwei Cheng, Zhaochun He
Summary: The study demonstrated that upregulation of beta Arr2 levels can improve symptoms and pathological damage in RA, attenuate inflammatory responses, and effectively alleviate ankle inflammation in CIA mice via the NF-kappa B/NLRP3 inflammasome pathway.
Article
Fisheries
ShaoQing Jian, JiangHe Leng, ZiYi Wen, HaiYang Luo, ChengXi Hu, ChunGen Wen, BaoQing Hu
Summary: In this study, a beta-arrestin homolog named Hc beta-arrestin was identified from Hyriopsis cumingii. It was found to regulate the immune response in mussels and negatively regulate the NF-kappa B pathway through interaction with HcTRAF6.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Cell Biology
Danila Del Rio, Ilenia Masi, Valentina Caprara, Francesca Spadaro, Flavia Ottavi, Raffaele Strippoli, Pilar Sandoval, Manuel Lopez-Cabrera, Ricardo Sainz de la Cuesta, Anna Bagnato, Laura Rosano
Summary: The study reveals that tumor-associated endothelin 1 (ET-1) can induce changes in mesothelial cells (MCs) by activating ETA and ETB receptors, promoting MC proliferation and genetic reprogramming towards mesenchymal transition. This enhances mesothelial cell migration, invasion, and SOC transmesothelial migration, contributing to the early progression of SOC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Mariko Takahashi, Chan-Wang J. Lio, Anaamika Campeau, Martin Steger, Ferhat Ay, Matthias Mann, David J. Gonzalez, Mohit Jain, Sonia Sharma
Summary: In this study, Sharma and colleagues identified DAPK3 as a positive regulator of the STING-interferon-beta activation pathway, essential for driving tumor-intrinsic innate immunity and tumor immune surveillance. Loss of DAPK3 expression or kinase activity impaired STING activation, reduced infiltration of immune cells, and attenuated the response to cancer chemo-immunotherapy. DAPK3 coordinates post-translational modification of STING and is critical for maintaining STING activation and immune response against tumors.
Article
Immunology
Shan-Shan Liu, Chang Liu, Xiao-Xi Lv, Bing Cui, Jun Yan, Yun-Xuan Li, Ke Li, Fang Hua, Xiao-Wei Zhang, Jiao-Jiao Yu, Jin-Mei Yu, Feng Wang, Shuang Shang, Ping-Ping Li, Zhi-Guang Zhou, Yang Xiao, Zhuo-Wei Hu
Summary: Recruitment of immune cells to the site of inflammation by CCL1 is crucial in inflammatory diseases, including pulmonary fibrosis. The study found that alveolar macrophages and CD4(+) T cells are major producers of CCL1, and targeting CCL1 expression can attenuate the pathology of PF.
Article
Cell Biology
Yanlin Cai, Yi Hu, Furong Yu, Wenjuan Tong, Shufen Wang, Shunliang Sheng, Jiayu Zhu
Summary: Globally, ovarian cancer ranks as the second most common cause of gynecologic-related cancer deaths among women, with a poor prognosis. Research has shown that downregulation of AHNAK in ovarian cancer cells can significantly inhibit cell growth and metastasis, partly by dampening the Canonical Wnt cascade. Therefore, AHNAK may serve as a biomarker and potential treatment target for ovarian cancer.
Article
Cell Biology
Ilenia Masi, Flavia Ottavi, Danila Del Rio, Valentina Caprara, Cristina Vastarelli, Sara Maria Giannitelli, Giulia Fianco, Pamela Mozetic, Marianna Buttarelli, Gabriella Ferrandina, Giovanni Scambia, Daniela Gallo, Alberto Rainer, Anna Bagnato, Francesca Spadaro, Laura Rosano
Summary: Dissemination of high-grade serous ovarian cancer (HG-SOC) in the omentum and intercalation into a mesothelial cell (MC) monolayer depends on functional alpha 5 beta 1 integrin (Int alpha 5 beta 1) activity. This study reveals a novel interactive signaling involving beta-arrestin1 (beta-arr1), the activation of the endothelin A receptor (ETAR) by endothelin-1 (ET-1), and the activation of Int alpha 5 beta 1, which promotes tumor cells towards invasive disease. The findings suggest that ETAR/beta-arr1 may regulate the functional pathway of Int alpha 5 beta 1 and could be considered in integrin-targeting programs.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Yi-Bo Wu, Shen-Yi Li, Jin-Yan Liu, Jia-Jia Xue, Jin-Fu Xu, Ting Chen, Tian-Yue Cao, Hui Zhou, Tian-Tian Wu, Chun-Lin Dong, Wei-Feng Qian, Long-Wei Qiao, Shun-Yu Hou, Ting Wang, Cong Shen
Summary: This study reveals the overexpression of long non-coding RNA NRSN2-AS1 in ovarian cancer and demonstrates its role in promoting ovarian cancer progression through stabilizing PTK2 and activating the beta-catenin pathway.
CELL DEATH & DISEASE
(2023)
Article
Plant Sciences
Ge Wang, Lu Li, Yan Li, Li-Hong Zhang
Summary: This study aimed to investigate the effects of toosendanin (TSN) on cisplatin (DDP) resistance in ovarian cancer (OC) and explore the underlying molecular mechanism. The results showed that TSN could reduce the DDP resistance in OC by modulating the miR-195/ERK/beta-catenin pathway, suggesting its potential as an effective agent to overcome clinical DDP resistance in OC.
Article
Anatomy & Morphology
Chunyi Ji, Lijian Chen, Miaoxian Yuan, Weixin Xie, Xinyi Sheng, Qiang Yin
Summary: This study investigates the mechanism of KDM1A-regulated hepatoblastoma (HB) development. The expression levels of KDM1A and DKK3 were analyzed in HB patients, and experiments were conducted on cell lines and nude mice to study the effects of knockdown of KDM1A and DKK3. The results suggest that KDM1A stimulates HB development by activating the Wnt/beta-catenin pathway through inhibition of DKK3 transcription.
Review
Oncology
Piera Tocci, Giovanni Blandino, Anna Bagnato
Summary: Recent studies have shown the interaction between ET-1 and the Hippo pathway, and the ET-1R/beta-arr1 axis triggers a transcriptional interplay involving YAP and mutant p53 proteins, which function in the oncogenic signaling network. Dual ET-1 receptor antagonists can interfere with the ET-1R/YAP/mutp53 signaling interplay, hindering metastasis and therapy resistance in tumors.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Rosanna Sestito, Piera Tocci, Celia Roman, Valeriana Di Castro, Anna Bagnato
Summary: This study reveals the role of endothelin-1/endothelin A receptor signaling in promoting metastasis in high-grade serous ovarian carcinoma cells by inducing the interaction between ZEB1 and YAP. It also demonstrates the involvement of Integrin-linked kinase signaling in activating the YAP/ZEB1 circuit, leading to cellular plasticity and invasion. Inhibition of this pathway with an ET-1R antagonist shows potential therapeutic effects in suppressing metastasis in vivo.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Rossella Loria, Valentina Laquintana, Stefano Scalera, Rocco Fraioli, Valentina Caprara, Italia Falcone, Chiara Bazzichetto, Marta Di Martile, Laura Rosano, Donatella Del Bufalo, Gianluca Bossi, Isabella Sperduti, Irene Terrenato, Paolo Visca, Silvia Soddu, Michele Milella, Gennaro Ciliberto, Rita Falcioni, Virginia Ferraresi, Giulia Bon
Summary: This study reveals that high expression of SEMA6A is associated with poor prognosis in BRAF-mutant melanoma and plays a critical role in promoting tumor aggressiveness and resistance to targeted therapies. SEMA6A may serve as a potential predictor for the efficacy of dual BRAF/MEK inhibition.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Nanoscience & Nanotechnology
Emanuele Limiti, Pamela Mozetic, Sara M. Giannitelli, Filippo Pinelli, Xiaoyu Han, Danila Del Rio, Franca Abbruzzese, Francesco Basoli, Laura Rosano, Stefano Scialla, Marcella Trombetta, Giuseppe Gigli, Zhenyu J. Zhang, Emanuele Mauri, Alberto Rainer
Summary: The emulsion/evaporation method is a pivotal approach for synthesizing nanogels for controlled drug delivery. However, it is limited in its use of polymers with phase-selective solubility in organic or aqueous solutions. This study presents an innovative emulsion-based strategy for producing nanogels from water-immiscible polymers hyaluronic acid (HA) and polyethyleneimine (PEI), which are chemically crosslinked. The nanogels obtained through this surfactant-free mixed emulsion exhibit low polydispersity, good colloidal stability, and high batch-to-batch reproducibility.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Annarita Stringaro, Stefano Serra, Alessandro Gori, Annarica Calcabrini, Marisa Colone, Maria Luisa Dupuis, Francesca Spadaro, Serena Cecchetti, Alberto Vitali
Summary: This study analyzed the cell specificity, cell viability effects, intracellular distribution, and immune cell response of a new peptide conjugate of Aloe-emodin in SKBR3 and A549 cell lines. The results showed that the conjugate was more effective at reducing cell viability than AE in both cell lines, with a nuclear localization in SKBR3 cells and cytoplasmic distribution in A459 cells.
Article
Cell Biology
Daniela Peruzzu, Zaira Boussadia, Federica Fratini, Francesca Spadaro, Lucia Bertuccini, Massimo Sanchez, Maria Carollo, Paola Matarrese, Mario Falchi, Francesca Iosi, Carla Raggi, Isabella Parolini, Alessandra Care, Massimo Sargiacomo, Maria Cristina Gagliardi, Katia Fecchi
Summary: In this study, the role of cholesterol transport in the endosomal degradative-secretory system was explored using the U18666A inhibitor in a metastatic human melanoma cell line (WM266-4). The results showed that U18666A induced a shift of Cav-1 from the plasma membrane to the endolysosomal compartment, affecting the formation and release of small extracellular vesicles (sEVs). The inhibitor also altered the protein composition of sEVs and reduced their transfer capacity on target cells.
Article
Cell Biology
Ilenia Masi, Flavia Ottavi, Danila Del Rio, Valentina Caprara, Cristina Vastarelli, Sara Maria Giannitelli, Giulia Fianco, Pamela Mozetic, Marianna Buttarelli, Gabriella Ferrandina, Giovanni Scambia, Daniela Gallo, Alberto Rainer, Anna Bagnato, Francesca Spadaro, Laura Rosano
Summary: Dissemination of high-grade serous ovarian cancer (HG-SOC) in the omentum and intercalation into a mesothelial cell (MC) monolayer depends on functional alpha 5 beta 1 integrin (Int alpha 5 beta 1) activity. This study reveals a novel interactive signaling involving beta-arrestin1 (beta-arr1), the activation of the endothelin A receptor (ETAR) by endothelin-1 (ET-1), and the activation of Int alpha 5 beta 1, which promotes tumor cells towards invasive disease. The findings suggest that ETAR/beta-arr1 may regulate the functional pathway of Int alpha 5 beta 1 and could be considered in integrin-targeting programs.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Piera Tocci, Celia Roman, Rosanna Sestito, Valeriana Di Castro, Andrea Sacconi, Ivan Molineris, Francesca Paolini, Mariantonia Carosi, Giovanni Tonon, Giovanni Blandino, Anna Bagnato
Summary: PARP inhibitors have changed the treatment paradigm of HG-SOC, but their efficacy is limited in patients with high TP53 mutation rates. The overexpression of the ET-1/ET1R axis in HG-SOC is associated with poor prognosis and inhibits the DNA damage response induced by PARP inhibitors. The ET-1R antagonist macitentan, in combination with olaparib, shows promising results in suppressing metastatic progression in HG-SOC patient-derived xenografts.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Manuela Priolo, Erika Zara, Francesca Clementina Radio, Andrea Ciolfi, Francesca Spadaro, Emanuele Bellacchio, Cecilia Mancini, Francesca Pantaleoni, Viviana Cordeddu, Luigi Chiriatti, Marcello Niceta, Emilio Africa, Corrado Mammi, Daniela Melis, Simona Coppola, Marco Tartaglia
Summary: RAC1 is a member of the RAS superfamily and plays a critical role in various cellular processes. Mutations in RAC1 are associated with a rare neurodevelopmental disorder called MRD48. Our study identified two individuals with previously unreported RAC1 mutations and demonstrated their pathogenicity, showing a gain-of-function effect. We also reviewed the clinical features of MRD48 and compared it with another neurodevelopmental disorder caused by mutations in the related gene RAC3.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Oncology
Giovanni Blandino, Roberto Dinami, Marco Marcia, Eleni Anastasiadou, Brid M. Ryan, Alina Catalina Palcau, Luigi Fattore, Giulia Regazzo, Rosanna Sestito, Rossella Loria, Ana Belen Diaz Mendez, Maria Chiara Cappelletto, Claudio Pulito, Laura Monteonofrio, George A. Calin, Gabriella Sozzi, Jit Kong Cheong, Ranit Aharonov, Gennaro Ciliberto
Summary: The 5th Workshop IRE on Translational Oncology was held in Rome (Italy) to discuss the significant progress made in the RNA field, focusing on diagnostic biomarkers and targeted therapies. Non-coding RNAs were highlighted for their potential in cancer diagnosis and therapy. The report collected presentations from scientists discussing recent oncology research and providing examples of RNA applications as biomarkers and therapeutics.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Meeting Abstract
Immunology
I. Farina, D. A. Covino, S. Sozzi, L. Catapano, F. Spadaro, S. Cecchetti, C. Purificato, M. C. Gauzzi, L. Fantuzzi
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Oncology
Danila Del Rio, Valentina Caprara, Ilenia Masi, Francesca Spadaro, Sara Giannitelli, Alberto Rainer, Anna Bagnato, Laura Rosano