Article
Biology
Greco Hernandez, Paula Vazquez-Pianzola
Summary: The evolutionary origin of eukaryotes drove the transition from prokaryotic-like translation to a more sophisticated eukaryotic translation through successive gene duplication of a single eIF4E gene. This resulted in the diversification of eIF4E functions in RNA metabolism across eukaryotes. In metazoans, eIF4E paralogs have different roles in various processes, and their expression and biochemical properties vary. Recent advances in understanding the functional diversification of eIF4E in metazoans, particularly in humans, fruit flies, and roundworms, are discussed in this review.
BIOLOGICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Ravi, Ajay Kumar, Shalmoli Bhattacharyya, Jogender Singh
Summary: Thiol antioxidants can modulate cellular physiology and activate the hypoxia response pathway, possibly through the production of hydrogen sulfide.
Article
Multidisciplinary Sciences
Ha Suk-Gyu, Boram Kang, Jong Suk Song
Summary: In this study, disinsertion of four extraocular rectus muscles in rabbits induced anterior segment ischemia, leading to increased concentrations of prostaglandin E2, hypoxia-inducible factor-1, and vascular endothelial growth factor. Subconjunctival injection of triamcinolone acetonide seemed to be effective in inhibiting the elevation of VEGF in anterior segment ischemia.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Christine E. Robbins, Bhumil Patel, Danielle L. Sawyer, Barrie Wilkinson, Brian K. Kennedy, Mark A. McCormick
Summary: Deletion of genes encoding ribosomal proteins extends lifespan in yeast by increasing translation of the transcription factor Gcn4. Inhibitors of cytosolic tRNA synthetase and mitochondrial tRNA synthetase also extend the lifespan of C. elegans, possibly acting through the same downstream pathway.
Article
Developmental Biology
Neda Masoudi, Eviatar Yemini, Ralf Schnabel, Oliver Hobert
Summary: Cells of the same type can be generated by distinct cellular lineages that originate in different parts of the developing embryo. The genes lin-32 and hlh-14 play key roles in the differential expression of neuronal lineages in Caenorhabditis elegans. Misregulation of terminal selector gene expression may result in neuron-to-neuron identity transformations in lin-32 mutants.
Article
Biology
Kelsie R. S. Doering, Xuanjin Cheng, Luke Milburn, Ramesh Ratnappan, Arjumand Ghazi, Dana L. Miller, Stefan Taubert
Summary: NHR-49, a nuclear hormone receptor in Caenorhabditis elegans, regulates a hypoxia response pathway that acts in parallel with HIF-1. In hypoxia, NHR-49 regulates a set of genes independent of HIF-1, including autophagy genes that promote hypoxia survival. NHR-67 acts as a negative regulator and HPK-1 acts as a positive regulator of the NHR-49 pathway.
Article
Biology
Florian Geisler, Sanne Remmelzwaal, Vera Jankowski, Ruben Schmidt, Mike Boxem, Rudolf E. Leube
Summary: Using Caenorhabditis elegans, researchers have identified the IF polypeptide IFB-2 as an efficient suppressor of structural and functional deficiencies in mutants that disrupt the organization of the intestinal IF cytoskeleton. This rescue capability is specific to IF isotypes and extends to mutants that disrupt the function of other cytoskeletal proteins. The findings provide strong evidence for the adverse consequences of deranged IF networks, with implications for diseases characterized by altered IF network organization.
Article
Biology
Thanh Thi Vuong-Brender, Sean Flynn, Yvonne Vallis, Mario de Bono
Summary: CAMTAs are ancient proteins expressed broadly in nervous systems, controlling neuronal CaM levels. Loss of CAMT-1, the sole C. elegans CAMTA, results in behavioral and neuronal Ca2+ signaling defects.
Article
Biology
Samuel J. P. Taylor, Laura Bel Borja, Flavie Soubigou, Jack Houston, Dhanya K. Cheerambathur, Federico Pelisch
Summary: Phosphorylation is an important post-translational modification used for regulating protein localization and activity. Polo-like kinase 1 (PLK-1) is crucial for mitotic and meiotic cell divisions, but its specific roles and regulation in meiosis have been unclear. In this study, using C. elegans oocytes, we demonstrate that PLK-1 is involved in spindle assembly, chromosome alignment and segregation, and polar body extrusion during meiosis I. We also uncover the mechanisms of PLK-1 recruitment to meiotic chromosomes. Our findings reveal the essential functions of PLK-1 in oocyte meiosis and provide insights into its targeting mechanisms.
Article
Biology
Omar Pena-Ramos, Lucia Chiao, Xianghua Liu, Xiaomeng Yu, Tianyou Yao, Henry He, Zheng Zhou
Summary: Autophagosomes play a crucial role in facilitating the degradation of apoptotic cells. They are recruited to the surfaces of phagosomes containing apoptotic cells and subsequently fuse with them, allowing the inner vesicle to enter the phagosomal lumen. Defects in autophagosome production result in significant defects in the degradation of apoptotic cells.
Article
Veterinary Sciences
Nicole Verhaar, Nicole de Buhr, Maren von Koeckritz-Blickwede, Katrin Duemmer, Marion Hewicker-Trautwein, Christiane Pfarrer, Franziska Dengler, Sabine Kaestner
Summary: This study investigated the expression and distribution of HIF in horse small intestinal ischemia. The results showed that HIF1 alpha plays a role in the intestinal response to ischemia in horses. However, the study did not identify an effect of postconditioning on HIF distribution or expression.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Cell Biology
Yunki Lim, Brandon Berry, Stephanie Viteri, Matthew McCall, Eun Chan Park, Christopher Rongo, Paul S. Brookes, Keith Nehrke
Summary: This study demonstrates that FNDC-1 plays a role in non-hypoxic mitochondrial quality control, with its loss leading to stress responses and metabolic remodeling dependent on atfs-1. These changes are prophylactic against subsequent HR injury.
Article
Biochemistry & Molecular Biology
Zohar Abergel, Maayan Shaked, Virendra Shukla, Zheng-Xing Wu, Einav Gross
Summary: The protein PITP-1 is found to be crucial for the rapid recovery of eating behavior in nematodes after hypoxia, whereas the neuroglobin GLB-5 plays a role in promoting the recovery of eating behavior. PITP-1 limits the availability of diacylglycerol (DAG), thereby controlling the activity of protein kinase C 1 (PKC-1) in neurons expressing mod-1. This mechanism enables worms to recover their eating behavior quickly after exposure to low oxygen levels.
Article
Cell Biology
Vanessa Brinkmann, Alfonso Schiavi, Anjumara Shaik, Daniel Ruediger Puchta, Natascia Ventura
Summary: The study reveals that modulators of AhR activity may impact aging and age-associated pathologies, with Caenorhabditis elegans proving to be a valuable model organism for investigating evolutionarily conserved functions and regulated processes of AhR.
Article
Immunology
Kerstin Muelling, Alexa Josephine Fischer, Elena Siakaeva, Mathis Richter, Sharareh Bordbari, Ilona Spyra, Christian Koester, Dirk M. Hermann, Matthias Gunzer, Ursula Felderhoff-Mueser, Ivo Bendix, Jadwiga Jablonska, Josephine Herz
Summary: This study found that neutrophils in the neonatal brain are rapidly activated after experimental hypoxia-ischemia, producing reactive oxygen species and showing an increased proportion of hyperactivated/aged cells compared to peripheral neutrophils. Delayed neutrophil depletion led to reduced cellular neurodegeneration and decreased microglial and astroglial activation, offering new possibilities for therapeutic approaches in post-hypoxic brain injury.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Review
Engineering, Biomedical
I. Lodoso-Torrecilla, J. J. J. P. van den Beucken, J. A. Jansen
Summary: Synthetic calcium phosphate ceramics are widely used for bone regenerative treatments due to their bioactivity and osteoconductive properties. Injectable CPCs are appealing for minimally invasive surgery and filling irregular bone defects, but their poor degradability can be addressed by introducing macroporosity to accelerate new bone formation.
ACTA BIOMATERIALIA
(2021)
Article
Dentistry, Oral Surgery & Medicine
Jinmeng Li, Abeer Ahmed, Tanika Degrande, Jeremie De Baerdemaeker, Abdulaziz Al-Rasheed, Jeroen Jjp van den Beucken, John A. Jansen, Hamdan S. Alghamdi, X. Frank Walboomers
Summary: The study showed that the application of a flexible titanium fiber mesh can promote new bone formation within the pores of the implant, increasing bone-to-implant contact and bone area percentage.
Article
Materials Science, Biomaterials
Jairo M. Cordeiro, Bruna E. Nagay, Caroline Dini, Joao G. S. Souza, Elidiane C. Rangel, Nilson C. da Cruz, Fang Yang, Jeroen J. J. P. van den Beucken, Valentim A. R. Barao
Summary: In this study, antimicrobial coatings were produced on commercially pure titanium using plasma electrolytic oxidation (PEO) with different copper sources. The CuAc coating exhibited optimal properties and antibacterial activity, as well as appropriate responses from human cells.
BIOMATERIALS ADVANCES
(2022)
Article
Engineering, Biomedical
Zhule Wang, Liebert Parreiras Nogueira, Havard Jostein Haugen, Ingrid C. M. Van der Geest, Patricia Caetano de Almeida Rodrigues, Dennis Janssen, Thom Bitter, Jeroen J. J. P. van den Beucken, Sander C. G. Leeuwenburgh
Summary: This study introduces a method to introduce porosity into polymethylmethacrylate (PMMA) cement for local delivery of chemotherapeutic drugs. The results showed that the cement released enough cisplatin within seven days to effectively kill osteosarcoma cells, while retaining comparable mechanical strength to intact tibias.
BIOACTIVE MATERIALS
(2022)
Review
Engineering, Biomedical
Marc Bohner, Yassine Maazouz, Maria-Pau Ginebra, Pamela Habibovic, Jonathan G. Schoenecker, Howard Seeherman, Jeroen J. J. P. van den Beucken, Frank Witte
Summary: This study aims to evaluate the hypothesis that sustained local ionic homeostatic imbalance (SLIHI) created by mineral formation during tissue calcification modulates inflammation to trigger heterotopic ossification (HO), and to provide insights for the design of cell-free, drug-free osteoinductive bone graft substitutes.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Yang Zhang, Marjolein Polman, Abdullah Faqeer Mohammad, Inge Hermens, Zhumei Zhuang, Huanan Wang, Jeroen J. J. P. van den Beucken
Summary: The coupling of bone resorption and bone formation is essential in the bone remodeling process. This study compared mouse and human osteoclasts and found that despite morphological and functional differences, their secretory factors have similar anabolic effects on the osteogenic differentiation of mesenchymal stem cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Cell & Tissue Engineering
Bart A. J. A. van Oirschot, Yang Zhang, Hamdan S. Alghamdi, Jairo M. Cordeiro, Bruna E. Nagay, Valentim A. R. Barao, Erica Dorigatti de Avila, Jeroen J. J. P. van den Beucken
Summary: This review highlights the significance of surface engineering in optimizing dental implants for bone integration and antibacterial efficacy, ensuring their success in challenging applications in the future.
TISSUE ENGINEERING PART A
(2022)
Article
Cell & Tissue Engineering
Bart A. J. A. Van Oirschot, Edwin J. W. Geven, Antonios G. Mikos, Jeroen J. J. P. Van den Beucken, John A. Jansen
Summary: Craniomaxillofacial bone defects present challenges in maxillofacial surgery and dentistry, and require effective bone graft materials. Synthetic bone substitutes have been developed, and efforts to optimize their properties are ongoing. This study describes a mini-pig mandibular bone defect model for evaluating bone regeneration using various bone substitute materials. The model allows for the creation of multiple critical-size bone defects in a single animal, making it suitable for preclinical evaluation.
TISSUE ENGINEERING PART C-METHODS
(2022)
Article
Materials Science, Biomaterials
Erica D. de Avila, Bruna E. Nagay, Marta M. A. . Pereira, Valentim A. . R. . Barao, Ana Claudia Pavarina, Jeroen J. J. P. van den Beucken
Summary: This study investigated the challenges involved in developing antimicrobial dental implant surfaces to fight peri-implantitis. The findings revealed that most of the research is still in the in vitro stage, with only a few articles testing clinical performance. This indicates that preclinical investigations still face many challenges before moving forward to a clinical setting.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Bart van Oirschot, Antonios G. Mikos, Qian Liu, Jeroen J. J. P. van den Beucken, John A. Jansen
Summary: The aim of this preclinical study was to test the applicability of CPC-PLGA-CMC as a bone substitute material for GBR procedures. The study showed that CPC-PLGA-CMC can be used as a bone-grafting material and stimulates bone regeneration to a comparable extent as with BioOss particles. Importantly, CPC-PLGA-CMC degrades faster compared to BioOss, is easier to apply into a bone defect, and does not need the use of an additional GBR membrane.
TISSUE ENGINEERING PART A
(2023)
Article
Dentistry, Oral Surgery & Medicine
Winston Adam Camargo, Jan Willem Hoekstra, John A. Jansen, Jeroen J. J. P. van den Beucken
Summary: In this study, the effect of alendronate (ALN) treatment on bone defect regeneration in osteoporotic rats was evaluated. The results showed that oral administration of ALN can enhance bone formation in the defects, increasing it to 10 times compared to the non-treated group. Additionally, Bio-Oss(R) was found to be an effective bone substitute, promoting bone formation in both healthy and osteoporotic conditions. The study highlights the beneficial effects of oral ALN in mitigating the adverse effects of osteoporosis on bone defect regeneration.
CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH
(2023)
Article
Cell & Tissue Engineering
Johanna F. A. Husch, Laura Coquelin, Nathalie Chevallier, Dorien Tiemessen, Egbert Oosterwijk, Rene van Rheden, Charlotte Woud, Jurriaan Vossen, Sander C. G. Leeuwenburgh, Jeroen J. J. P. van den Beucken
Summary: This study compared the osteogenic differentiation capacity between stromal vascular fraction (SVF) and adipose tissue-derived mesenchymal stromal cells (ATMSCs). The results showed that SVF demonstrated superior osteogenic capacity compared to ATMSCs.
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Cell & Tissue Engineering
Bart van Oirschot, Jeroen J. J. P. van den Beucken, Antonios G. G. Mikos, John A. A. Jansen
Summary: This study aimed to test the effectiveness of CPC-PLGA mixed with poly(lactic-co-glycolic acid) as bone graft material for lateral bone augmentation. The results showed significant degradation of CPC-PLGA and BioOss particles after 4 and 12 weeks of implantation, accompanied by bone formation. However, statistical analysis indicated that BioOss performed better in promoting bone formation compared to CPC-PLGA. Therefore, neither treatment achieved clinically meaningful alveolar ridge augmentation.
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Materials Science, Multidisciplinary
Qian Liu, Irene Lodoso-Torrecilla, Raquel Klein Gunnewiek, Harry R. Harhangi, Antonios G. Mikos, Laura van Niftrik, John A. Jansen, Lili Chen, Jeroen J. J. P. van den Beucken
Summary: In this study, a system was established to achieve different release profiles of the antibiotic drug doxycycline from calcium phosphate cements (CPCs) by adjusting the formulation. The CPC formulations showed appropriate handling properties and released doxycycline with antibacterial activity against Staphylococcus aureus. In vivo implantation experiments demonstrated the effectiveness of the CPC system in local antibacterial therapy.
Article
Engineering, Biomedical
Johanna F. A. Husch, Laura Coquelin, Nathalie Chevallier, Natasja W. M. van Dijk, Sander C. G. Leeuwenburgh, Jeroen J. J. P. van den Beucken
Summary: Through our experiment, we found that neither human macrophages nor osteoclasts are capable of inducing ectopic bone formation in the human body.
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2023)