Article
Endocrinology & Metabolism
Jianshu Yang, Fang Wang, Desong Zhao, Shoutang Lu
Summary: This study found that PAK6 plays a promoting role in gastric cancer and may serve as an indicator for gastric cancer, guiding clinical practice.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Oncology
Xinchun Xu, Xiaofeng Xuan, Jieru Zhang, Hui Xu, Xiaomei Yang, Ling Zhang, Yuanjie Zhao, Hong Xu, Dawei Li
Summary: PSMD7 is highly expressed in NSCLC tissues and is associated with tumor progression, lymph node invasion, and survival outcomes in LUAD patients. Knockdown of PSMD7 inhibits cell proliferation and induces cell cycle arrest, senescence, and apoptosis, suggesting its potential as a therapeutic target in LUAD.
Article
Plant Sciences
Xingyu Lv, Yuqi Lin, Xi Zhu, Xiujun Cai
Summary: In this study, it was found that Isoalantolactone (IAL), a compound derived from Inula helenium L., has inhibitory effects on gallbladder cancer (GBC). IAL can inhibit the proliferation, migration, and invasion of GBC cells, promote cell apoptosis, and exert its effects by inhibiting the ERK signaling pathway. Animal experiments also confirmed that IAL can suppress the growth of gallbladder tumors.
PHARMACEUTICAL BIOLOGY
(2023)
Article
Oncology
Takeharu Nakamura, Yoshihiro Nishikawa, Masahiro Shiokawa, Haruhiko Takeda, Masataka Yokode, Shimpei Matsumoto, Yuya Muramoto, Sakiko Ota, Hiroyuki Yoshida, Hirokazu Okada, Takeshi Kuwada, Saiko Marui, Tomoaki Matsumori, Takahisa Maruno, Norimitsu Uza, Yuzo Kodama, Etsuro Hatano, Hiroshi Seno
Summary: The loss of function mutations of ELF3 are frequently observed in gallbladder cancer (GBC), and ELF3 suppresses GBC development through downregulation of EREG/EGFR/mTORC1 signaling pathway. Inhibition of EGFR/mTORC1 could be a potential therapeutic option for GBC with ELF3 mutation.
JOURNAL OF PATHOLOGY
(2023)
Article
Oncology
Thanigaivelan Kanagasabai, Guoliang Li, Tian Huai Shen, Nataliya Gladoun, Mireia Castillo-Martin, Sherly I. Celada, Yingqiu Xie, Lakendria K. Brown, Zaniya A. Mark, Josiah Ochieng, Billy R. Ballard, Carlos Cordon-Cardo, Samuel E. Adunyah, Renjie Jin, Robert J. Matusik, Zhenbang Chen
Summary: The study found that miR-21 promotes the progression of prostate cancer by activating the IRS1/SREBP-1 axis, and targeting the miR-21/SREBP-1 signaling pathway may be a novel strategy for controlling the malignancy of prostate cancer.
Article
Biochemistry & Molecular Biology
Silvia Yumnam, Min Cheol Kang, Seung Hyun Oh, Hak Cheol Kwon, Jin Chul Kim, Eun Sung Jung, Choong Hwan Lee, Ai-Young Lee, Jong-Ik Hwang, Sun Yeou Kim
Summary: Suppressing dihydrolipoyl dehydrogenase (DLD) increases intracellular ROS production, decreases mitochondrial membrane potential, induces autophagic cell death, and significantly inhibits melanoma growth and proliferation. Furthermore, DLD suppression alters energy metabolism in melanoma cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Ganghua Yang, Qinhong Xu, Yong Wan, Lei Zhang, Lin Wang, Fandi Meng
Summary: This study demonstrated the crucial role of circ-CSPP1 in hepatocellular carcinoma (HCC) by regulating the expression of miR-493-5p and HMGB1, thereby suppressing HCC development both in vitro and in vivo.
CELLULAR SIGNALLING
(2021)
Article
Chemistry, Medicinal
Lishan Ouyang, Jiaqi Li, Xiaonan Chen, Huiming Huang, Yingying Tian, Xingxing Li, Daoran Pang, Xuejiao Wei, Jinxin Xie, Longyan Wang, Dongxiao Liu, Pengfei Tu, Jun Li, Zhongdong Hu
Summary: Chinese dragon's blood ethyl acetate extract (CDBEE) has demonstrated anti-gastric cancer effects by suppressing proliferation and metastasis, inhibiting epithelial-mesenchymal transition, inducing apoptotic and autophagic cell death, and showing no obvious toxicity or side effects. Therefore, CDBEE may be a promising candidate drug for the treatment of gastric cancer.
PHYTOTHERAPY RESEARCH
(2023)
Article
Cell Biology
Zhitao Chen, Yanhui Song, Peipei Li, Wei Gao
Summary: This study found that glutamate ionotropic receptor NMDA type subunit 2D (GRIN2D) is upregulated in lung adenocarcinoma (LUAD) and inhibits cell growth, migration, glycolysis, and gluconeogenesis through inhibition of the E2F signaling pathway.
CELLULAR SIGNALLING
(2023)
Article
Cell Biology
Wei Wei, Xiaoliang Zhao, Jiang Liu, Zhenfa Zhang
Summary: This study reveals that silencing of LINC00665 suppresses LUAD progression through targeting the miR-181c-5p/ZIC2 axis. High expression levels of LINC00665, ZIC2, HOXA1, and HOXA11, along with low expression of miR-181c-5p, are closely associated with poor prognosis of LUAD patients. Knockdown of LINC00665 induced significant inhibitions in cell viability, clone formation, invasion, and tumorigenesis in LUAD cells, while downregulation of miR-181c-5p neutralized these effects.
Article
Oncology
Yuzhu Di, Yanan Jiang, Xiuyun Shen, Jing Liu, Yang Gao, Huimin Cai, Xiaoli Sun, Dandan Ning, Bing Liu, Jiaji Lei, Shizhu Jin
Summary: Esophageal cancer (EC) is a common human cancer with high morbidity. MicroRNAs (miRNAs) play a key role in various cancers, including EC. The study found that upregulation of miR-135b-5p in EC samples, and its inhibition suppresses proliferation, invasion, and migration while promoting apoptosis of cancer cells. Additionally, the miR-135b-5p/TXNIP pathway contributes to the anti-tumor effect of cisplatin (DDP) in EC.
FRONTIERS IN ONCOLOGY
(2021)
Article
Microbiology
Sung-Su Kim, Kwang-Soo Shin
Summary: The study revealed the role of the promoter region of SFTPD in fungal-infected cells, with the HSF1 transcription factor playing a crucial role in decreasing SFTPD expression. Inhibiting the binding of HSF1 to the promoter region of SFTPD is identified as an important step to prevent conidia infection.
Article
Biochemistry & Molecular Biology
Chao Li, Juanjuan Cai, Weifeng Liu, Zhenzhen Gao, Guogang Li
Summary: circ-STK39 expression is upregulated in pancreatic cancer tissues and cells, indicating its potential role in pancreatic cancer progression. Downregulation of circ-STK39 inhibits proliferation and migration of pancreatic cancer. Furthermore, TRAM2 and miR-140-3p are identified as downstream targets of circ-STK39, and TRAM2 overexpression reverses the effects of miR-140-3p on migration, proliferation, and epithelial-mesenchymal transition (EMT) in pancreatic cancer.
Article
Medicine, Research & Experimental
Mei Jin, Chunlei Yuan, Sijia Duan, Bin Zeng, Lingjuan Pan
Summary: The present study investigated the effects of ACC expression interference on the malignant progression of breast cancer cells. It was found that knockdown of ACC expression significantly decreased cell viability and migration, and increased apoptosis in MCF-7 and MDA-MB-231 breast cancer cells. In vivo experiments also showed that ACC knockdown effectively reduced tumor volume and promoted tumor cell apoptosis. These findings suggest that downregulation of ACC expression may significantly reduce the malignant progression of breast cancer and could be considered as a potential therapeutic target.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Cell Biology
Tian Hao, Yi Yang, Juan He, Jia Bai, Yongjian Zheng, Zhanpeng Luo
Summary: This study found that hsa_circ_0062270 was significantly upregulated in melanoma cells and its knockdown inhibited melanoma proliferation and invasion, while promoting apoptosis. Further research revealed that hsa_circ_0062270 regulated melanoma cell growth through CDC45. Additionally, hsa_circ_0062270 interacted with RNA-binding protein EIF4A3, suggesting its potential role in melanoma development. These findings provide biological mechanisms for the use of hsa_circ_0062270 as a biomarker for melanoma and a potential therapeutic target.
HISTOLOGY AND HISTOPATHOLOGY
(2022)
Article
Biochemical Research Methods
Daiki Asakawa
Summary: MALDI-ISD induces selective cleavage of C-alpha-C peptide bonds with an oxidizing matrix, resulting in specific peptide fragments formed through hydrogen abstraction by the matrix.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Serina Fukui, Eiji Sugiyama, Hajime Mizuno, Iwao Sakane, Daiki Asakawa, Kazumi Saikusa, Yuki Nishiya, Yuri Amano, Kentaro Takahara, Daisuke Higo, Toshimasa Toyo'oka, Kenichiro Todoroki
Summary: A rapid method for separating dl-2-hydroxyglutaric acid using a single analytical instrument was presented, demonstrating potential for clinical analysis. This method is more efficient and effective compared to traditional methods, showing promise for future applications in clinical settings.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Oncology
Atsushi Saitou, Yoshihiro Hasegawa, Naoki Fujitani, Shigeru Ariki, Yasuaki Uehara, Ukichiro Hashimoto, Atsushi Saito, Koji Kuronuma, Kunio Matsumoto, Hirofumi Chiba, Motoko Takahashi
Summary: The MET receptor is associated with resistance to tyrosine kinase inhibitors in non-small cell lung cancer. This study explores the roles of N-glycans in the proteolytic processing of MET and HGF-induced MET signaling. The findings suggest that N-glycans of MET affect the status and function of the receptor in a site-specific manner.
Review
Biochemistry & Molecular Biology
Motoko Takahashi, Yoshihiro Hasegawa, Kento Maeda, Masato Kitano, Naoyuki Taniguchi
Summary: Glycosylation of cell surface receptors plays a crucial role in regulating signal transduction. This review focuses on the role of glycosyltransferases involved in N-glycan modification, specifically the effects of branching and elongation on signaling by cell surface receptors. The review also discusses the involvement of these enzymes in EMT/MET programs related to differentiation, cancer development, progression, and therapy resistance.
GLYCOCONJUGATE JOURNAL
(2022)
Article
Biochemical Research Methods
Daiki Asakawa, Kazumi Saikusa
Summary: Small molecules with polar functional groups, like substituted phenethylamines, are commonly analyzed using liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI). However, the ESI of substituted phenethylamines often results in intense fragment ion signals, making it difficult to identify these compounds. By using computational methods and suitable functionals, the dissociation efficiency of substituted phenethylamines can be evaluated, providing reliable results.
JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Chemistry, Multidisciplinary
Daiki Asakawa, Hidenori Takahashi, Shinichi Iwamoto, Koichi Tanaka
Summary: The reaction between even-electron peptide ions and hydrogen atoms has a high transition state barrier, requiring the use of hot hydrogen atoms to initiate the reaction and increase the internal energy of the resulting product. This reaction leads to the formation of oxygen- and carbon-centered peptide radicals, which undergo radical-induced fragmentation on sub-picosecond and sub-millisecond time scales, respectively.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Michiko Tajiri, Hiroto Aoki, Atsuko Shintani, Kaori Sue, Satoko Akashi, Yoshiaki Furukawa
Summary: Cu/Zn-superoxide dismutase (SOD1) can bind a non-stoichiometric amount of copper and zinc ions, forming a homodimer with a stochastic combination of subunits. Correct metallation of SOD1 is important for amyotrophic lateral sclerosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemical Research Methods
Daiki Asakawa, Takuya Hosokai, Yasuo Nakayama
Summary: Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) with a reducing matrix is initiated by hydrogen transfer and the use of matrices containing aniline groups achieves more efficient MALDI-ISD. Conversion of phenol group to aniline group decreases the ionization energy of the matrix solids. Lower ionization energy of the matrix leads to efficient cleavage at peptide bonds by MALDI-ISD. Electron association with the peptide is proposed as the initial step of the MALDI-ISD process. Fragmentation of the peptide anion radical strongly depends on the gas-phase acidity of the matrix used. The proposed mechanism helps to better understand the MALDI-ISD process.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Kyoka Hoshi, Mayumi Kanno, Mitsunari Abe, Takenobu Murakami, Yoshikazu Ugawa, Aya Goto, Takashi Honda, Takashi Saito, Takaomi C. Saido, Yoshiki Yamaguchi, Masakazu Miyajima, Katsutoshi Furukawa, Hiroyuki Arai, Yasuhiro Hashimoto
Summary: CSF protein levels may be associated with neurodegenerative diseases, particularly Alzheimer's disease. Increased CSF protein levels in CN subjects are positively correlated with AD markers, while correlations decline in MCI and AD patients with AD markers.
Article
Biochemical Research Methods
Daiki Asakawa, Kazumi Saikusa
Summary: We propose using substituted benzyl ammonium species as thermometer ions to characterize the internal energy distribution of ions produced by electrospray ionization. Experimental results show that the activation of benzyl ammonium species preferentially generates benzyl cations. Calculations reveal that the threshold energies of fragmentation for the tested model ions are significantly lower than those of conventional 4-substituted benzylpyridinium thermometer ions. Furthermore, the internal energies of the ions depend on the radiofrequency voltage of the ion funnel.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Daiki Asakawa, Kenichiro Todoroki, Hajime Mizuno
Summary: This study investigated the fragmentation patterns of histamine and histidine under electrospray ionization (ESI) using both experimental and theoretical approaches. The results showed that histamine undergoes NH3 loss, producing bicyclo and spiro compounds, while protonated histidine primarily loses H2O. The study also demonstrated that the analysis of these fragmentation ions can facilitate the identification of metabolites derived from aromatic amino acids.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Review
Pharmacology & Pharmacy
Noritaka Hashii, Michiko Tajiri, Akiko Ishii-Watabe
Summary: Translation: In the development of therapeutic monoclonal antibodies (mAbs), it is important to characterize the modifications causing structural heterogeneity. The multi-attribute method (MAM), based on peptide mapping, has emerged as a new quality control method for mAbs. It allows for simultaneous monitoring of modification rates and quantitative testing.
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
(2022)
Article
Biochemical Research Methods
Noriyoshi Manabe, Shiho Ohno, Kana Matsumoto, Taiji Kawase, Kenji Hirose, Katsuyoshi Masuda, Yoshiki Yamaguchi
Summary: The determination of glycan structures is crucial for understanding the relationship between structure and function of glycans and glycoconjugates. Mass spectrometry is an effective method for analyzing glycan structures due to its high sensitivity and mass resolution. In this study, we propose a rapid and precise method for identifying N-glycan structures using ultraperformance liquid chromatography-connected ion mobility mass spectrometry (UPLC/IM-MS). We validate this methodology by examining 71 pyridylaminated (PA-) N-linked oligosaccharides, including isomeric pairs. By establishing a database of m/z, CCS index, and normalized retention time (GU), we provide a practical strategy for structural analysis of N-linked glycans.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Multidisciplinary Sciences
Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Mizuki Takemoto, Kunio Hirata, Mika Hirose, Rika Oi, Tatsuya Kobayashi, Kenichi Miyoshi, Rie Aruga, Tatsuhiko Yokoyama, Shizuka Katagiri, Hiroaki Matsuura, Kenji Iwasaki, Takayuki Kato, Mika K. Kaneko, Yukinari Kato, Michiko Tajiri, Satoko Akashi, Osamu Nureki, Yohei Hizukuri, Yoshinori Akiyama, Terukazu Nogi
Summary: Site-2 proteases are a conserved family of intramembrane proteases that regulate signal transduction and maintain proteostasis by cleaving transmembrane substrates. The crystal structures of inhibitor-bound forms of bacterial site-2 proteases were determined, revealing that conformational changes in the RseP domains expose the substrate-binding site. Mutational analysis showed that the conformational changes are mediated by a conserved electrostatic linkage between transmembrane and peripheral membrane-associated domains. In vivo cleavage assays supported the model that the substrate transmembrane helix is unwound and clamped by the active center of RseP for efficient cleavage.
Article
Chemistry, Multidisciplinary
Michiko Tajiri, Shunsuke Imai, Tsuyoshi Konuma, Keiko Shimamoto, Ichio Shimada, Satoko Akashi
Summary: We developed a platform using native mass spectrometry to investigate the responses of G-protein-coupled receptors (GPCRs) to agonists and antagonists. By utilizing mini-G(s) and nanobody80 (Nb80) that mimic the G & alpha; interface of GPCRs, we successfully observed the ligand-bound human & beta;(2) adrenergic receptor (h & beta;(2)AR). The ratio of h & beta;(2)AR-mini-G(s) or -Nb80 complexes observed in mass spectra showed a strong correlation with agonist/antagonist efficacy obtained from a cell-based assay.
