Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Tong Luo, Jiacheng Li, Yao He, Hui Liu, Zhiwei Deng, Xi Long, Qingqing Wan, Jiacheng Ding, Zan Gong, Yanjing Yang, Shian Zhong
Summary: In this study, we developed a novel CRISPR-based biosensor by combining CRISPR/Cas12a with Au-nanobeacon, which allows for direct and rapid detection of nucleic acids in complex biological fluids with high sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Huimin Hao, Ye Li, Bin Yang, Shuyan Lou, Zihua Guo, Weiyi Lu
Summary: In this study, a unique DNA probe with Hill-type cooperativity was developed based on toehold-mediated strand displacement processes, enabling accurate discrimination of single-nucleotide variants. The probe exhibited thermodynamic advantages for specificity, forming mismatch bubbles in the presence of single-nucleotide variants. The probe's feasibility was successfully demonstrated and its design allowed accurate discrimination of single-nucleotide variants, including insertions, mutations, and deletions, with Hill-type cooperativity serving as a novel strategy for DNA probe design.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Helena de Puig, Rose A. Lee, Devora Najjar, Xiao Tan, Luis R. Soekensen, Nicolaas M. Angenent-Mari, Nina M. Donghia, Nicole E. Weckman, Audrey Ory, Carlos F. Ng, Peter Q. Nguyen, Angelo S. Mao, Thomas C. Ferrante, Geoffrey Lansberry, Hani Sallum, James Niemi, James J. Collins
Summary: The miSHERLOCK is a low-cost CRISPR-based POC diagnostic platform that can rapidly detect SARS-CoV-2 and its variants with high sensitivity in just 1 hour. This modular system can be quickly adapted to different viruses and variants, and is complemented by a smartphone application for output interpretation and result reporting.
Article
Chemistry, Analytical
Qiuyuan Lin, Yuanwei Cao, Guobin Han, Wen Sun, Wenhao Weng, Hui Chen, Haoyi Wang, Jilie Kong
Summary: We report a general and programmable diagnostic platform that combines mesophilic Clostridium perfringens Argonaute (CpAgo) with exponential isothermal amplification (EXPAR) for the rapid and sensitive detection of multiple miRNAs with single-nucleotide discrimination in one pot. The CpAgo-based One-Pot (COP) assay achieved a limit of detection of 1 zMmiRNA within 30 min and showed superior analytical performance in distinguishing colorectal cancer patients from healthy individuals. This programmable, one-pot, multiplex, rapid, and specific strategy holds great promise in scientific research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Meiting Yang, Yidan Tang, Lijuan Qi, Sicai Zhang, Yichen Liu, Baiyang Lu, Jiaxue Yu, Kun Zhu, Bingling Li, Yan Du
Summary: A portable and accurate nucleic acid detection system was developed to address the urgent need for on-site virus screening and infection control. It utilizes ultrasensitive isothermal amplification to achieve rapid and sensitive detection of SARS-CoV-2 RNA, providing a cost-effective solution for emergency detection of the virus.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Siyu Chen, Bo Gong, Cong Zhu, Chunyang Lei, Zhou Nie
Summary: Nucleic acid molecules possess attractive properties for biosensing and bioimaging due to their high designability and structural predictability. CRISPR-Cas systems, with high programmability and flexibility, recognize and cleave nucleic acid targets and have been engineered as multifunctional tools. Integration of nucleic acids with CRISPR-Cas systems has advanced biosensing and bioimaging methodologies, holding great promise in biochemical research and clinical diagnosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Robert E. Jinkerson, Joseph A. Russo, Casandra R. Newkirk, Andrea L. Kirk, Richard J. Chi, Mark Q. Martindale, Arthur R. Grossman, Masayuki Hatta, Tingting Xiang
Summary: Photosynthesis is not necessary for symbiosis establishment, but its impact depends on specific cnidarian-Symbiodiniaceae relationships. UV mutagenesis can generate photosynthetic mutants capable of infecting hosts in the absence of photosynthesis.
Article
Chemistry, Analytical
Nan Zong, Ying Gao, Yong Chen, Xueqing Luo, Xingyu Jiang
Summary: A centrifugal microfluidic platform was developed for combined pretreatment and molecular diagnosis. Multiple signal enhancement systems were used to avoid false-negative results. The platform successfully detected and genotyped hepatitis B virus from whole blood. Compared to traditional diagnostic processes, the platform significantly reduced time consumption and the need for professional labor.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Food Science & Technology
Tai-Yong Kim, Xiaoning Zhu, Se-Min Kim, Jeong-A Lim, Min-Cheol Lim, Ke Luo
Summary: Viruses are major pathogens in food poisoning, and the development of foodborne virus detection technologies for the entire food distribution chain is crucial for food safety. Polymerase chain reaction (PCR) is the gold standard method for monitoring food contamination by viruses, but it is time-consuming and vulnerable to inhibitors in food samples. Optical and electrical readout biosensors based on nucleic acid isothermal amplification technology and nanomaterials have emerged as alternatives, offering portability and on-site operation. However, the complexity of food components and lack of stability studies on residual food components limit the practical application of biosensors as a universal detection method.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Chemistry, Multidisciplinary
Yiru Wang, Yan Fei, Tao Yang, Zisheng Luo, Yanqun Xu, Bin Su, Xingyu Lin
Summary: Nucleic acid detection is a highly valued tool in various fields such as life science, agriculture, food safety, and environmental surveillance due to its sensitivity, specificity, and simplicity. After the global COVID-19 pandemic, there has been increasing importance placed on exploring ultrafast nucleic acid amplification methods to accelerate the detection process and control the spread of infectious diseases. This review focuses on the advances in nanotechnology research for ultrafast nucleic acid amplification, including nanofluidics, nanoporous materials, and nanoparticles. The recent progress in these nanotechnologies for sample pretreatment, enzymatic amplification acceleration, and rapid heating/cooling processes is summarized. The challenges and future applications of ultrafast nucleic acid amplification are also discussed.
Article
Biophysics
Yuanyuan Zhu, Jian Wu, Qingli Zhou
Summary: Functional DNA (fDNA) refers to DNA molecules that have specific binding ability or catalytic function, with advantages of simple synthesis, low cost, low toxicity, high chemical stability, recognition specificity, and biocompatibility. However, the limited sensitivity of fDNA sensors to trace targets is a major challenge, especially when there is low affinity between fDNA and the targets. Various nucleic acid signal amplification strategies (NASAS) have been explored to improve the limit of detection of fDNA. This review summarizes four NASAS (hybridization chain reaction, entropy-driven catalysis, rolling circle amplification, CRISPR/Cas system) and their corresponding design principles, as well as the principle and application of fDNA sensors integrated with signal amplification strategies for the detection of non-nucleic acid targets. The main challenges and application prospects of NASAS-integrated fDNA biosensing system are also discussed.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yan Zhao, Xiaomeng Li, Mei-Hao Xiang, Feng Gao, Fengli Qu, Mingfang Li, Limin Lu
Summary: A novel photoelectrochemical biosensor based on an enzyme-free nucleic acid dual-amplification strategy combined with a mimic enzyme has been developed for ultrasensitive detection of miRNA-21. The biosensor achieves outstanding performance with an ultralow detection limit of 33 aM and a wide quantification range from 100 aM to 1 nM. This study opens a new avenue for ultrasensitive detection of miRNAs and has potential applications in clinical and biochemical samples.
Article
Chemistry, Analytical
Tong-Lin Hou, Liang Zhu, Xiao-Long Zhang, Ya-Qin Chai, Ruo Yuan
Summary: In this study, an intelligent multiregion linear DNA walker (MLDW) was developed for ultrasensitive detection of miRNA. By using the rolling-circle amplification mediated by target miRNA, the MLDW achieved a significantly improved reaction rate. The reaction could be completed within 30 minutes, which is at least 4 times faster than traditional DNA walkers. As a proof of concept, the high-efficiency MLDW was applied to an electrochemical biosensing platform and successfully detected target miRNA-21 with a detection limit of 36 aM. This innovative design of DNA walker provides new insights for constructing functional DNA nanodevices and promoting the research of nucleic acid signal amplification.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Marco A. Villanueva, Stephanie Barnay-Verdier, Fabrice Priouzeau, Paola Furla
PHOTOSYNTHESIS RESEARCH
(2015)
Article
Marine & Freshwater Biology
Ludmila Sromek, Didier Forcioli, Rafal Lasota, Paola Furla, Katarzyna Tarnowska-Marini, Maciej Wolowicz, Anne Chenuil
JOURNAL OF MOLLUSCAN STUDIES
(2016)
Article
Ecology
Patricia Ventura, Michael D. Jarrold, Pierre-Laurent Merle, Stephanie Barnay-Verdier, Thamilla Zamoum, Riccardo Rodolfo-Metalpa, Piero Calosi, Paola Furla
MARINE ECOLOGY PROGRESS SERIES
(2016)
Article
Plant Sciences
S. Roberty, P. Furla, J. -C. Plumier
PLANT CELL AND ENVIRONMENT
(2016)
Article
Ecology
Patricia Ventura, Michael D. Jarrold, Pierre-Laurent Merle, Stephanie Barnay-Verdier, Thamilla Zamoum, Riccardo Rodolfo-Metalpa, Piero Calosi, Paola Furla
MARINE ECOLOGY PROGRESS SERIES
(2016)
Article
Biodiversity Conservation
Cedric Mallien, Barbara Porro, Thamilla Zamoum, Caroline Olivier, Jorg Wiedenmann, Paola Furla, Didier Forcioli
SYSTEMATICS AND BIODIVERSITY
(2018)
Article
Biology
Alexis Pey, Jerome Cataneo, Didier Forcioli, Pierre-Laurent Merle, Paola Furla
COMPTES RENDUS BIOLOGIES
(2013)
Article
Biotechnology & Applied Microbiology
Stephanie Barnay-Verdier, Diane Dall'Osso, Nathalie Joli, Juliette Olivre, Fabrice Priouzeau, Thamilla Zamoum, Pierre-Laurent Merle, Paola Furla
Article
Biochemistry & Molecular Biology
Vincent Dani, Philippe Ganot, Fabrice Priouzeau, Paola Furla, Cecile Sabourault
Article
Multidisciplinary Sciences
Leila Ezzat, Pierre-Laurent Merle, Paola Furla, Alexandre Buttler, Christine Ferrier-Pages
Article
Biochemistry & Molecular Biology
Barbara Porro, Thamilla Zamoum, Cedric Mallien, Benjamin C. C. Hume, Christian R. Voolstra, Eric Rottinger, Paola Furla, Didier Forcioli
Summary: This study investigated the flexibility of symbiont acquisition in the Mediterranean snakelocks sea anemone Anemonia viridis, revealing that the composition of within-host-symbiont populations is more dependent on geographical origin of the hosts rather than lineage. The similarities in host-symbiont communities were greater among different genets, suggesting a capacity for horizontal acquisition. The mixed-mode transmission strategy in A. viridis may explain the large phenotypic plasticity observed in this anemone.
Article
Cell Biology
Clara Fricano, Eric Roettinger, Paola Furla, Stephanie Barnay-Verdier
Article
Biochemistry & Molecular Biology
Alexis Pey, Thamilla Zamoum, Richard Christen, Pierre-Laurent Merle, Paola Furla