Article
Plant Sciences
Min Jiang, Xiaoying Wang, Bin Lv, Yujie Lu, Xianghui Ma, Wenjuan Liu, Gang Bai, Xiumei Gao
Summary: The deposition of psoralen in the liver is the primary cause of liver damage, which is related to the inhibition of CYPs and GSTs activities leading to the depletion of GSH. Co-administration of GSH and psoralen is an effective approach to prevent liver injury in clinical settings.
Article
Biochemistry & Molecular Biology
Lulu Han, Lin Zhou, Hongzhu Zou, Meng Yuan, Yan Wang
Summary: Anthocyanins, the important chromogenic substances responsible for the coloration of flowers, are transported and accumulated predominantly by PsGSTF3 gene. Silencing of PsGSTF3 gene leads to decreased anthocyanin accumulation and affects structural genes in the anthocyanin biosynthesis pathway. Heterologous overexpression of PsGSTF3 enhances anthocyanin accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
John Pleinis M., Loga Norell, Radha Akella, John M. Humphreys, Haixi He, Qifei Sun, Fen Zhang, Jason Sosa Pagan, Daryl Morrison E., Jeffrey Schellinger N., Laurie Jackson K., Elizabeth Goldsmith J., Aylin Rodan R.
Summary: The study reveals that high potassium can independently inhibit the activity of fruit fly and mammalian WNK kinases by directly affecting the kinase, regardless of chloride ions.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Environmental Sciences
Derek Simonsen, Jacob Heffelfinger, David M. Cwiertny, Hans-Joachim Lehmler
Summary: The metabolism of the herbicide safener benoxacor in Rhesus monkey subcellular fractions was investigated in this study, with findings showing that the metabolism was primarily mediated by carboxylesterase and cytochrome P450 enzymes. Differences were observed in the metabolism between male and female monkeys, and enantioselective metabolism by glutathione-S-transferases and CYPs may have an impact on the toxicity of benoxacor.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Li-Wei Meng, Meng-Ling Chen, Guo-Rui Yuan, Li-Sha Zheng, Wei Dou, Yu Peng, Shu-Xia Zhang, Jin-Jun Wang
Summary: The oriental fruit fly, Bactrocera dorsalis, is a damaging insect pest that has developed resistance to various chemical insecticides. This study identified several glutathione S-transferases (GSTs) in B. dorsalis and characterized their relationships with five insecticides. It was found that an antenna-abundant BdGSTd8 responded to four different classes of insecticides. Further analysis confirmed that BdGSTd8 is primarily located in the antenna and has the ability to enhance cell viability by interacting with specific insecticides. These findings enhance our understanding of GST characteristics in B. dorsalis and their role in detoxification.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Lei Xue, Xiaorong Huang, Zehuang Zhang, Qihua Lin, Qiuzhen Zhong, Yun Zhao, Zhongshan Gao, Changjie Xu
Summary: The study revealed that MrGST1 in Chinese bayberry is closely related to anthocyanin accumulation and is responsible for anthocyanin transport. Additionally, co-expression of MrGST1 with MrMYB1.1 and MrbHLH1 significantly enhances pigmentation in tobacco leaves.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Gandhali Bapat, Javed Mulla, Chaitali Labade, Onkar Ghuge, Vaijayanti Tamhane, Smita Zinjarde
Summary: In this study, HaGST-8 was successfully immobilized on glutaraldehyde-activated APTES functionalized silica nanoparticles to form SiAPT-HaGST-8 nano-conjugates, which showed higher specific activity compared to free HaGST-8. The pH range for activity of the nano-conjugates was extended from 8.0 to 9.0, while the free enzyme had a pH optimum of 9.0. Both forms of the enzyme had an optimum temperature of 30 degrees C, with improved stability for the nano-conjugates lasting 10 days longer than the free enzyme. These findings suggest the potential use of silica nanoparticle-based systems for stable enzyme conjugation and effective removal of pesticides from aqueous media.
ENVIRONMENTAL RESEARCH
(2022)
Article
Plant Sciences
Seth A. Strom, Aaron G. Hager, Jeanaflor Crystal T. Concepcion, Nicholas J. Seiter, Adam S. Davis, James A. Morris, Shiv S. Kaundun, Dean E. Riechers
Summary: The resistance of waterhemp to the herbicide S-metolachlor involves a combination of Phase I and Phase II metabolic activities, specifically the O-demethylation reaction conferring resistance. Greater activities of GSTs and P450s were observed in resistant populations compared to sensitive populations, leading to increased metabolism of S-metolachlor and the formation of resistant metabolites.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xingang Li, Yuanting Pang, Yiwang Zhong, Zhandong Cai, Qibin Ma, Ke Wen, Hai Nian
Summary: In this study, a tau-like glutathione transferase family gene named GmGSTU23 was identified in soybean, which showed a concentration-time-specific expression pattern in response to salt stress. Transgenic lines with GmGSTU23 displayed enhanced salt tolerance, root length, and fresh weight compared to the wild type. Changes in glutathione pools and associated enzyme activity were observed, indicating that GmGSTU23 enhances the activity of glutathione transferase to scavenge reactive oxygen species and glutathione, thereby conferring enhanced tolerance to salt stress in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Elsa M. Materon, Flavio M. Shimizu, Kevin Figueiredo dos Santos, Gustavo F. Nascimento, Vananelia P. N. Geraldo, Osvaldo N. Oliveira Jr, Ronaldo C. Faria
Summary: This study investigated the interaction between anticancer drugs and glutathione-s-transferase using Langmuir monolayers as models for tumor cell membranes. The results suggest that glutathione-s-transferase and its cofactor hinder the effects of doxorubicin and cisplatin on the mechanical properties of the monolayers, supporting the hypothesis of drug inactivation by GST.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Tomaz Trobec, Kristina Sepcic, Monika Cecilija Zuzek, Jerneja Kladnik, Nina Podjed, Catarina Cardoso Pascoa, Iztok Turel, Robert Frangez
Summary: The study demonstrates the significance of different structural elements of organoruthenium complexes for their inhibitory activities against ChEs and GSTs. Some compounds show potential as drugs for further testing.
Article
Biochemistry & Molecular Biology
Jianqin Zhang, Wen Ma, Fei Yin, Yoonseong Park, Kun Yan Zhu, Xueyao Zhang, Xuemei Qin, Daqi Li
Summary: This study investigated the ability of two GSTEs to metabolize insecticides in the locust. One of the GSTEs was found to be able to metabolize malathion and DDT, and participate in the detoxification of the acetylcholinesterase inhibitor malathion. These findings are important for understanding insecticide resistance mechanisms and managing insect populations in the field.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Chemistry, Physical
Onur Akyildirim, Hilal Medetalibeyoglu, Ercan Oguz, Abdulmelik Aras, Abdurrahman Atalay, Adem Korkmaz, Murat Beytur, Fikret Turkan, Haydar Yuksek
Summary: The reaction of 1,2,4-triazole derivatives with morpholine and formaldehyde leads to the synthesis of heterocyclic N-Mannich bases. The structure of the compounds was determined using spectroscopic techniques. The synthesized compounds showed different enzyme inhibition activities against Glutathione S-transferase.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biochemistry & Molecular Biology
Mohammed Hamed Alqarni, Ahmed Ibrahim Foudah, Magdy Mohamed Muharram, Nikolaos E. Labrou
Summary: This study investigated the interaction between dyes and hGSTA1-1, finding that PBMX-R displayed higher affinity and specifically inactivated the enzyme with negative cooperativity. It was also found to be a competitive inhibitor of glutathione and a mixed-type inhibitor of CDNB, suggesting it could be a useful probe for assessing the drugability of the enzyme in future drug-design efforts.