期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 213, 期 19, 页码 3305-3310出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.041954
关键词
Belostoma; extra-oral digestion; lysophosphatidylcholine; paralysis; phospholipase A2; saliva
类别
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Financiadora de Estudos e Projetos (FINEP)
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)
- International Foundation for Science (IFS)
- Fondazione CARIPARO
- Telethon [GGP06133]
- Regione Veneto Programma Biotech III
Lysophosphatidylcholine (LPC) is a major bioactive lipid that is enzymatically generated by phospholipase A(2) (PLA(2)). Previously, we showed that LPC is present in the saliva of the blood-sucking hemipteran Rhodnius prolixus and modulates cell-signaling pathways involved in vascular biology, which aids blood feeding. Here, we show that the saliva of the predator insect Belostoma anurum contains a large number of lipids with LPC accounting for 25% of the total phospholipids. A PLA(2) enzyme likely to be involved in LPC generation was characterized. The activity of this enzyme is 5-fold higher in Belostoma saliva than in other studied hemipterans, suggesting a close association with the predator feeding habits of this insect. Belostoma employs extra-oral digestion, which allows for ingestion of larger prey than itself, including small vertebrates such as amphibians and fish. Therefore, prey immobilization during digestion is essential, and we show here that Belostoma saliva and B. anurum saliva purified LPC have paralytic activity in zebrafish. This is the first evidence that lysophospholipids might play an important role in prey immobilization, in addition to contributing to blood feeding, and might have been an evolutionary acquisition that occurred long before the appearance of hematophagy in this animal group.
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