期刊
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
卷 32, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1756-9966-32-3
关键词
Interleukin-17; Receptor; Hepatic stellate cell; Prognosis; Hepatocellular carcinoma
类别
资金
- National Key Sci-Tech Special Project of China [2012ZX10002010-001-002]
- National Natural Science Foundation of China [81071707, 81071995, 81030038]
- Open Project of the State Key Laboratory of Oncogene and Related Gene [90-09-03]
- Doctoral Fund of the Ministry of Education of China [200802460019]
Background: Hepatocellular carcinoma (HCC) is a typical malignancy in a background of chronic inflammation. Th17 cells (a major source of IL-17) constitute crucial components of infiltrating inflammatory/immune cells in HCC and can amplify inflammatory response via binding to interleukin-17 receptor (IL-17R). Thus, we investigated the expression and clinical significance of IL-17 and IL-17 receptor family cytokines in HCC. Methods: The expression and prognostic value of IL-17 and IL-17R (A-E) were examined in 300 HCC patients after resection. Six Th17 associated cytokines in serum (n = 111) were quantified using enzyme-linked immunosorbent assays. Phenotypic features of IL-17(+) CD4(+) T cells were determined by flow cytometry analysis. Results: High expression of intratumoral IL-17 and IL1-7RE were significantly associated with poorer survival (p = 0.016 and < 0.001, respectively) and increased recurrence (both P < 0.001) of HCC patients. Moreover, intratumoral IL-17, individually or synergistically with IL-17RE, could predict HCC early recurrence and late recurrence. Also, peritumoral IL-17RE showed the prognostic ability in HCC (P < 0.001 for OS/TTR). Furthermore, expression levels of Th17 associated cytokines including IL-6, -22, -17R and TNF-alpha were increased in serum of HCC patients compared to haemangioma patients. Importantly, activated human hepatic stellate cells induced in vitro expansion of IL-17(+) CD4(+) T cells. Conclusions: High expression of IL-17 and IL-17RE were promising predictors for poor outcome of HCC patients. The protumor power of IL-17 producing CD4(+) T cells was probably involved in the crosstalk with different types of inflammatory/immune cells in HCC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据