期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 137, 期 1, 页码 396-402出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2011.05.036
关键词
Atractylodes macrocephala Koidzumi; Obesity; 3T3-L1 adipocyte; Oil Red O staining; Phospho-Akt; Perilipin
资金
- Kyung Hee University [20071480]
Ethnopharmacological relevance: Atractylodes macrocephala Koidzumi (AMK) is an herbal medicine traditionally used for treatment of abdominal pain, gastrointestinal disease, obesity, and related complications. Aim of the study: We investigated the effects and molecular mechanism of AMK rhizome water extract on 3T3-L1 adipogenesis and an animal model of obesity. Materials and methods: To study the effect of AMK on adipogenesis in vitro, differentiating 31341 cells were treated every two days with AMK at various concentrations (1-25 mu g/ml) for eight days. Oil Red 0 staining was performed to determine the lipid accumulation in 3T3-L1 cells. To elucidate the inhibitory mechanism of AMK on adipogenesis, phosphorylation levels of Akt and expression of perilipin, were analyzed by Western blotting. AMK was administered orally to high fat diet (HFD)-induced obese rats to confirm its effect in vivo. Results: AMK inhibited 3T3-L1 adipocyte differentiation in a dose-dependent manner without cellular toxicity. Phospho-Akt expression was highly decreased by AMK treatment, whereas there was no significant change in perilipin expression. AMK administration significantly reduced the body weight of rats fed a HFD. Plasma triglyceride levels were significantly lower in the AMK-treated HFD group than those in the HFD control group or normal diet (ND) group, although serum total, HDL- and LDL-cholesterol levels did not differ between the groups. Conclusion:These results demonstrate an inhibitory effect of AMK on adipogenesis through reduction of an adipogenic factor, phospho-Akt. AMK had a beneficial effect, reducing body weight gain in a HFD-induced animal model of obesity. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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