期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 133, 期 3, 页码 1008-1014出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2010.11.029
关键词
Cissus quadrangularis; Nitric oxide; Heme oxygenase-1; Inducible nitric oxide synthase; Macrophage
资金
- Faculty of Science, Burapha University
- Center for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, Thailand
Aim of the study: Cissus quadrangularis (family: Vitaceae) has been widely used in traditional herbal medicine for the treatment of hemorrhoids, gastric ulcers and bone healing. In the present study, we determined the anti-inflammatory activity and the molecular mechanism of the ethyl acetate extract of Cissus quadrangularis stem (CQE) in LPS-stimulated RAW 264.7 macrophage cells. Materials and methods: The inhibitory effect of CQE on LPS-induced nitric oxide (NO) production was evaluated in conditioned media. Cell viability was monitored by MTT assay. Protein and mRNA expressions were determined by RT-PCR and Western blotting analysis, respectively. Results: CQE potently inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophage cells in a dose-dependent manner. The mRNA and protein expressions of inducible nitric oxide synthase (iNOS) were suppressed also by CQE as was p65 NF-kappa B nuclear translocation. Further study demonstrated that CQE by itself induced heme oxygenase-1 (HO-1) gene expression at the protein and mRNA levels in dose- and time-dependent manner. In addition, the inhibitory effects,of CQE on NO production were abrogated by a HO-1 inhibitor, zinc protoporphyrin IX (ZnPP). Conclusions: Collectively, these results suggest that CQE exerts an anti-inflammatory effect in macrophages, at least in part, through the induction of HO-1 expression. These findings provide the scientific rationale for anti-inflammatory therapeutic use of Cissus quadrangularis stem. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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