期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 124, 期 1, 页码 87-97出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2009.04.007
关键词
Hematopoiesis; Thrombopoiesis; Megakaryocytopoiesis; Thrombopoietin; Apoptosis; Radix angelicae sinensis; Radix astragali; Danggui Buxue Tang (DBT)
Ethnopharmacological relevance: A decoction containing Radix angelicae sinensis and Radix astragali (Danggui Buxue Tang, DBT) has been used to raise the Qi and nourish the Blood. However, its effects on haematopoiesis and particularly thrombopoiesis have not been studied. Aims: This study aims to examine the effects of DBT on hematopoiesis and thrombopoiesis. Materials and methods: A myelosuppression mouse model was treated with DBT (10 mg/kg/day). Peripheral blood cells from DBT and thrombopoietin-treated samples were counted on days 0, 7,14 and 21. Then CFU assays were used to determine the effects of DBT on the megakaryocytic progenitor cells and other lineages. Last, analyses of annexin V, caspase-3. and mitochondrial membrane potential were conducted in megakaryocytic cell line M-07e. Results: Morphological examination showed that DBT treatment significantly increased the recovery of the megakaryocytic series. DBT significantly enhanced the platelet recovery and CFU-MK formation in vivo. DBT significantly promoted CFU-MK and CFU-F formation. Last, we observed the antiapoptotic effects of DBT on M-07e cells. Conclusion: DBT might promote haematopoiesis and thrombopoiesis in the mouse model through (i) directly promoting the growth of megakaryocytes: (ii) indirectly promoting the growth of bone marrow stromal cells; (iii) inhibiting apoptosis of megakaryocytes. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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