期刊
JOURNAL OF ENVIRONMENTAL SCIENCES
卷 24, 期 5, 页码 963-968出版社
SCIENCE PRESS
DOI: 10.1016/S1001-0742(11)60820-6
关键词
attP/attB integration; E. coli biosensor; GFP; mercury
资金
- Central Institute of Fisheries Education, Mumbai
A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of X phage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P <= 0.05) for each concentration of inducing Hg(H) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples.
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