Article
Food Science & Technology
Gabor Endre, Babett Edit Nagy, Daniel Hercegfalvi, Csenge Kasuba, Csaba Vagvolgyi, Andras Szekeres
Summary: Aflatoxins (AFs) are secondary metabolites that cause diseases in animals and humans. Regulations set concentration limits for AFs in EU food and feed products, requiring pure forms for reference standards. Improvements were made to a liquid-liquid chromatographic method to enhance purification and increase the yield of pure AFs. Through a scale-up process, approximately 2.2 g of AFs could be purified using 8.2 L of solvent in a 250 mL rotor, while 7.8 g of AFs could be prepared using 31 L of solvent in a 1000 mL rotor.
Article
Food Science & Technology
Vishal Kumar, Ashutosh Bahuguna, Jong Suk Lee, Ankur Sood, Sung Soo Han, Hyang Sook Chun, Myunghee Kim
Summary: Bacillus albus YUN5, isolated from doenjang, showed high degradation capabilities for aflatoxin B1 (AFB1) and aflatoxin G1 (AFG1). The cell-free supernatant (CFS) of B. albus YUN5 exhibited the highest degradation efficiency, while negligible degradation was observed in other fractions. Optimal degradation conditions were determined to be 55℃, pH 7-10, and salt concentration of 0-20%. Liquid chromatography-mass spectroscopy analysis revealed the target sites of degradation. The application of B. albus YUN5 in doenjang fermentation showed better reduction of AFB1 and AFG1 compared to without B. albus YUN5.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Jie Zheng, Ge-Ge Gong, Shu-Jian Zheng, Yu Zhang, Yu-Qi Feng
Summary: Carboxylic metabolites, an important class of metabolites in mammals, were successfully labeled using N-methylphenylethylamine (MPEA) to enhance detection sensitivity and chromatographic retention. The optimized method achieved high coverage analysis of carboxylated metabolites in HepG2 cells, identifying a total of 403 potential metabolites.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jonas Carneiro Cruz, Israel Donizeti de Souza, Caroline Fernandes Grecco, Eduardo Costa Figueiredo, Maria Eugenia Costa Queiroz
Summary: Bioanalysis faces challenges in sample preparation due to the complexity of biological samples, but recent trends focus on miniaturization, automation, and high-throughput performance. Column switching liquid chromatography, as a multidimensional method, can improve sensitivity and accuracy, reduce organic solvent consumption, and enhance sample handling efficiency.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Chemistry, Analytical
Ali Mohebbi, Mahboob Nemati, Mir Ali Farajzadeh, Mohammad Reza Afshar Mogaddam, Farzaneh Lotfipour
Summary: A temperature-induced counter-current homogenous liquid-liquid extraction procedure has been proposed for the isolation of aflatoxins from fruit chip samples. The method demonstrates great repeatability, low limits of determination and quantification, and high extraction recoveries.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Energy & Fuels
Jian-Fang Xu, Fei Wang, Xing Fan, Turghun Muhammad, Peter N. Kuznetsov, Ya-Ya Ma
Summary: This study investigated the thermal dissolution process of soft coal and hard coal using different organic solvents. By employing off-line prep-HPLC, the soluble fractions were reliably separated and enriched, leading to the identification of heavy aromatic compounds not detected by GC/MS. The differences in composition between the two coals were elucidated, providing theoretical guidance for efficient coal utilization and mine safety.
Article
Chemistry, Analytical
Marta Passamonti, Chiem de Roos, Peter J. Schoenmakers, Andrea F. G. Gargano
Summary: In this study, polymerization mixture was optimized to synthesize poly(acrylamide-co-N,N'-methylenebisacrylamide) monolithic stationary phases for HILIC of intact proteins. The monolithic structure exhibited a permeable and polar stationary phase suitable for HILIC. The monolith showed superior separation performance and lower ion interaction compared to silica-based particulate amide phase, enabling successful hyphenation with high-resolution mass spectrometry in HILIC of intact proteins.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Zhixing Gu, Xi Nie, Ping Guo, Yuehua Lu, Bo Chen
Summary: An analytical method using HPLC with diode array and evaporative light scattering detectors was established for the simultaneous determination of hydrophobic and hydrophilic compounds in the dried rhizome of Atractylodes macrocephala Koidz. The method provides a new choice for the quality control of traditional Chinese medicines and health foods.
Article
Chemistry, Analytical
Xiaomei Chen, Liulin Wei, Qian Liu, Jingchan Zhao
Summary: A novel method utilizing SLSC-ME-HPLC-UV was developed for preconcentrating trace bisphenols in environmental water, achieving high extraction efficiency through optimized conditions.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Yoapoing Shi, Dipendu Mandal, Zhenbin Zhang, Yufen Zhao
Summary: We have developed a new protocol for determining D/L amino acids using a chiral phosphinate derivatization. (RP)-L-Menthyl phenylphosphinate successfully labeled eighteen pairs of amino acids except for Cys, and the chirality of amino acids was discriminated by 31P NMR. Seventeen pairs of amino acids were separated in 45 minutes using a C18 column, with resolution values ranging from 2.01 to 10.76. The lowest limit of detection was 10 pM in parallel reaction monitoring, highlighting the potential of chiral phosphine oxides in future chiral metabolomics.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Saeed Lesan, Hamid Mirzaei, Jalil Khandaghi, Mohammad Reza Afshar Mogaddam, Afshin Javadi
Summary: In this study, a sample pretreatment method based on the combination of deep eutectic solvent-based minia-turized pressurized liquid extraction and deep eutectic solvent-based dispersive liquid-liquid microextraction was introduced. This method was used for the extraction and preconcentration of aflatoxins from rice samples prior to their determination by high performance liquid chromatography-fluorescent detector. The results showed that the proposed method has the advantages of low limits of detection (0.02-0.07 ng g-1) and quantification (0.07-0.23 ng g-1), as well as high extraction recoveries (69-82%).
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Wei Li, Lian Xu, Xue Zhang, Zihan Ding, Xingxing Xu, Xiaojun Cai, Yanying Wang, Chunya Li, Dong Sun
Summary: A photoelectrochemical aptasensor was developed for detecting Aflatoxin B1 (AFB1) in food using Er-MOF nanoballs assembled with gold nano-particles. The aptasensor exhibited high selectivity, reproducibility, and accuracy, and showed good performance in the determination of AFB1 in real corn samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Danial Shamsaei, Shu-An Hsieh, Iran Ocana-Rios, Saxon J. Ryan, Jared L. Anderson
Summary: This study introduces the first smartphone-based fluorescence detector for HPLC with cost-effective and customizable flow cells, offering a potential solution for the analysis of co-eluting compounds. Smartphone detection in HPLC provides tremendous opportunities in further miniaturizing chromatographic instrumentation while offering high sensitivity and can be expanded to other mechanisms of detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Ali Mohebbi, Mahboob Nemati, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh, Farzaneh Lotfipour
Summary: In this study, a vitamin-based metal-organic framework was used for the first time as a sorbent in dispersive micro-solid-phase extraction. The synthesized framework exhibited good adsorption capacity for aflatoxins, and the proposed method showed satisfactory extraction recoveries, low limits of detection, and good repeatability. Additionally, the green synthesis and low usage of adsorbents and organic solvents contributed to the environmental friendliness of the method.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Ozgur Golge, Ahmet Koluman, Bulent Kabak
FOOD ANALYTICAL METHODS
(2018)
Article
Biotechnology & Applied Microbiology
Tugce Kanik, Bulent Kabak
JOURNAL OF FOOD SAFETY
(2019)
Article
Food Science & Technology
Ozgur Golge, Bulent Kabak
Article
Mycology
Ayca Kulahi, Bulent Kabak
MYCOTOXIN RESEARCH
(2020)
Article
Biochemical Research Methods
Emrah Gormez, Ozgur Golge, Bulent Kabak
Summary: A study quantifying six polar residues in pomegranate samples from Turkey found that phosphonic acid and fosetyl-Al were detected, with some samples exceeding the EU MRL for fosetyl-Al. Other polar residues were not detected in any samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Applied
Emrah Gormez, Ozgur Golge, Orhan Dincay, Bulent Kabak
Summary: The analysis of highly polar pesticide residues in fruits and vegetables, especially black figs intended for export from Turkey, showed that 16.8% of samples had detectable levels of ethephon but below EU MRL levels. The study demonstrated that the QuPPe method used for analysis met the EU MRL standards for ethephon, fosetyl-Al, and phosphonic acid residues.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Food Science & Technology
Ozgur Golge, Turan Liman, Bulent Kabak
Summary: This study validated a fast method for simultaneous analysis of 365 LC-amenable and 142 GC-amenable pesticides in hen eggs using LC-MS/MS and GC-MS/MS, based on QuEChERS extraction. The method showed good recoveries and RSDs for most pesticide residues, with low LOQs. Application of the validated method to 100 egg samples from caged hens did not quantify any pesticides.
FOOD SCIENCE OF ANIMAL RESOURCES
(2021)
Article
Food Science & Technology
Koray Sahin, Adnan Bozdogan, Kurban Yasar, Tulin Eker, Bulent Kabak
Summary: This study investigates the impact of different extraction processes on the transfer of aflatoxins from peanuts to oils. AFB(1) and AFB(2) were detected in all peanut samples collected, but no aflatoxins were found in the oils obtained by industrial application method. The ratios of AFB(1) and AFB(2) transitions to oils varied depending on the extraction method used.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Chemistry, Applied
Ozgur Golge, Emirhan Yenisehirlioglu, Bulent Kabak
Summary: The incidence of patulin in spirit drinks and apple juices collected in Turkey was analyzed. Patulin was found at higher levels in apple juice samples compared to spirit drink samples, and it was not detected in vodka samples. The dietary exposure of adults to patulin was found to be low.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2022)
Article
Biochemistry & Molecular Biology
Emrah Gormez, Ozgur Golge, Miguel Angel Gonzalez-Curbelo, Bulent Kabak
Summary: This study aimed to analyze twelve highly polar pesticide residues in cherry samples intended for export from Turkey. A total of 16,022 cherry samples from 2018-2020 harvests in four production areas of Turkey were analyzed using a modified method and liquid chromatography-tandem mass spectrometry. Results showed that 28.4% of the cherry samples contained phosphonic acid, with amounts up to 77.7 mg kg(-1). Additionally, 14.4% of the samples had residues that exceeded the European Union maximum residue level. However, long-term exposure to these pesticides through the consumption of sweet cherries is not a cause for concern.
Article
Food Science & Technology
Bilge Deveci, Ozgur Golge, Bulent Kabak
Summary: This study monitored pesticide residues in 200 samples of green leafy vegetables purchased from shops, markets, and bazaars in Corum Province, Turkey. It was found that 35% of the samples had no quantifiable residues, while 43 residues belonging to 24 different chemical classes were detected in 130 green leafy vegetables. The residue levels exceeded European Union Maximum Residue Levels (EU MRLs) in 46% of the samples, with dill, rocket, and parsley being the most frequently affected.
Article
Food Science & Technology
Sule Aslantas, Ozgur Golge, Miguel Angel Gonzalez-Curbelo, Bulent Kabak
Summary: This study aimed to monitor multi-class pesticide residues in lemon fruits commercialized in Turkey and investigate the transmission of residues from the outer surface of the fruit into its juice. The results showed the presence of various pesticide residues in lemon fruits, but no residues were detected in the juice.
Review
Food Science & Technology
Miguel Angel Gonzalez-Curbelo, Bulent Kabak
Summary: Dried fruits are popular and nutritious snacks, but they can be contaminated with mycotoxins, mainly aflatoxins and ochratoxin A. The production of mycotoxins is influenced by various factors, and the geographic origin of dried fruits can result in different contamination levels. By consolidating research from different regions and stages of the supply chain, a global understanding can be gained and regions with higher contamination risks can be identified.
Article
Biochemistry & Molecular Biology
Emrah Gormez, Ozgur Golge, Miguel Angel Gonzalez-Curbelo, Bulent Kabak
Summary: The increased demand for plant production products is a significant concern due to the current focus on high agricultural yields in citrus production. This study aimed to analyze pesticide residues in satsuma mandarins, including highly polar compounds. Samples were collected over three consecutive years in Turkey, and the residues were analyzed using QuEChERS extraction and LC-MS/MS and GC-MS/MS determination methods. The results showed the presence of various residues, including two highly polar substances, with some samples exceeding the maximum residue levels (MRL). Phosphonic acid and spirotetramat were the most frequently found pesticides, and their concentrations varied in the mandarin samples. These findings are important for assessing pesticide presence, identifying potential risks, and ensuring consumer safety.
Article
Environmental Sciences
Celile Ozcicek-Dolekoglu, Isil Var
FRESENIUS ENVIRONMENTAL BULLETIN
(2019)