Article
Agriculture, Dairy & Animal Science
Lingwei Sun, Mengqian He, Caifeng Wu, Shushan Zhang, Jianjun Dai, Defu Zhang
Summary: Soy lecithin (SL) and its nanoparticles (nano-SL) can effectively prevent spermatozoa cryodamage during cryopreservation, with a concentration of 1.0% nano-SL showing the most positive impact on post-thawing semen quality and fertility in roosters. The addition of nano-SL reduced oxidative stress levels, improved sperm motility and mitochondrial activity, and enhanced fertility and hatchability in rooster semen after artificial insemination.
Review
Veterinary Sciences
Ana Patricia Alves Leao, Alexandre Vinhas de Souza, Nelson Fijamo Mesquita, Luciano Jose Pereira, Marcio Gilberto Zangeronimo
Summary: This systematic review evaluated the potential benefits of antioxidant enrichment of rooster semen extenders. The findings suggest that antioxidants can reduce oxidative stress and improve fertilizing capacity for roosters.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Reproductive Biology
R. Masoudi, S. Esmaeilkhanian, M. Sharafi, Z. Abdollahi, V Jafari, A. Hatefi, F. Zarei, N. Asadzadeh, A. Sadeghipanah, H. J. Barfourooshi, M. H. Banabazi
Summary: The study demonstrated that adding 1 and 2mM of CYS to the extender improved rooster semen quality during cold storage by enhancing motility, membrane functionality, viability, and reducing lipid peroxidation. Semen samples supplemented with 1 and 2mM CYS showed higher fertility rates after 23 hours of cold storage compared to the control group.
Article
Agriculture, Dairy & Animal Science
Shaimaa K. Hamad, Ahmed M. Elomda, Yanyan Sun, Yunlei Li, Yunhe Zong, Jilan Chen, Ahmed O. Abbas, Farid K. R. Stino, Ali Nazmi, Gamal M. K. Mehaisen
Summary: This study aims to evaluate the impact of different concentrations (3%, 6%, or 9%) of dimethylacetamide (DMA) as a cryoprotectant on post-thawed sperm quality, motility, antioxidant-biomarkers, and the expression of anti-freeze related genes. The results showed that 3% DMA group maintained higher post-thawed sperm quality compared to the other tested groups, with improvements in motility, viability, and antioxidant enzyme activity. Additionally, certain anti-freeze related genes were upregulated in the 3% DMA group.
Article
Agriculture, Dairy & Animal Science
Theerapat Kheawkanha, Vibuntita Chankitisakul, Pachara Thananurak, Maruay Pimprasert, Wuttigrai Boonkum, Thevin Vongpralub
Summary: This study aimed to investigate the effect of adding different concentrations of serine to the Thai native rooster semen extender on semen quality and fertility potential during solid storage at 5 degrees C for up to 120 h. The results showed that the extender supplemented with gelatin and 4 mM serine successfully preserved the rooster semen for up to 72 h with good semen quality. This study provides valuable information for the prolonged storage of cold semen in roosters.
Article
Veterinary Sciences
Ngassa Julius Mussa, Wuttigrai Boonkum, Vibuntita Chankitisakul
Summary: Smallholder farmers often face the problem of low fertility and hatchability after artificial insemination. This study found that the amount of semen production in Thai native roosters is correlated with sperm concentration and quality. Chickens producing higher semen volumes exhibit superior sperm quality compared to those producing lower volumes. These findings suggest that semen production could be used as a tool for selecting Thai native roosters for breeding, particularly for smallholder farmers who lack access to specialized semen evaluation tools.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Lei Shi, Yunlei Li, Jingwei Yuan, Hui Ma, Panlin Wang, Aixin Ni, Pingzhuang Ge, Chao Chen, Dongli Li, Yanyan Sun, Jilan Chen
Summary: The study found that early photostimulation can advance sexual maturation in rooster breeders, but it does not affect semen quality or hatching performance in adulthood.
Article
Agriculture, Dairy & Animal Science
Zabihollah Nemati, Parviz Dehgani, Amir Karimi, Saeid Amirdahri, Davoud Kianifard
Summary: The study evaluated the effects of dietary supplementation of ginger root on semen quality, blood chemistry, immune response, testicular histology, and reproductive performance in Ross-308 breeder roosters. The results showed that ginger root supplementation improved sperm motility and seminal antioxidant status, as well as increased the spermiation index and seminal tube spermatozoids number. However, there was no significant improvement in fertility and hatchability rate.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Article
Biology
Napapach Chauychu-noo, Pachara Thananurak, Wuttigrai Boonkum, Thevin Vongpralub, Vibuntita Chankitisakul
Summary: Supplementation of diets with 0.3, 0.6, and 0.9 ppm selenium was found to improve the quality of fresh and frozen-thawed semen in roosters, increasing sperm viability and normal morphology while decreasing lipid peroxidation and enhancing fertility. Different selenium levels also impacted sperm concentration and quality parameters, with 0.6 ppm selenium recommended for optimal improvements in both fresh and frozen semen.
Article
Agriculture, Dairy & Animal Science
Fereshteh Sadat Rezaie, Maryam Hezavehei, Mohsen Sharafi, Abdolhosein Shahverdi
Summary: The study investigated the antioxidant effects of resveratrol during rooster semen cryopreservation and evaluated the changes in AMP-activated protein kinase expression. Results showed that RSV-0.1 improved total motility, membrane integrity, and sperm mitochondria activity, while reducing apoptotic changes. Different concentrations of RSV did not affect malondialdehyde concentration, velocity, or linearity, but a dose-dependent increase in AMP-activated protein kinase expression was observed.
Article
Agriculture, Dairy & Animal Science
Tingqi Zhu, Wenjie Liang, Yuehua He, Binbin Zhang, Cong Liu, Dongxue Wang, Lekun Deng, Donghua Li, Wenting Li, Fengbin Yan, Yadong Tian, Ruili Han, Xiangtao Kang, Zhuanjian Li, Ruirui Jiang, Guirong Sun
Summary: The reproductive performance of breeder roosters is economically important, but their semen quality declines with age. Induced molting has been used to extend their use, showing positive effects on body weight, testicular weight, semen quality, and reproductive performance. Transcriptome sequencing analysis revealed genes involved in the regulation of actin cytoskeleton, including SRC and ROCK2, which play roles in spermatid connection disruption and senescence atrophy in the testes of aged roosters.
Article
Reproductive Biology
Nanwei Ye, Zengpeng Lv, Zhenwu Huang, Ying Cheng, Quanwei Wei, Fangxiong Shi
Summary: This study investigated the effects of dietary folic acid supplementation on the reproductive performance of aged broiler breeder roosters. The results showed that dietary supplementation with folic acid significantly improved semen quality and spermatogenesis in aged roosters. Furthermore, folic acid supplementation improved testicular autophagy through the mTOR signaling pathway and altered histone methylation in the testis.
Article
Infectious Diseases
Eva Tvrda, Michaela Petrovicova, Filip Benko, Michal Duracka, Jan Kovac, Tomas Slanina, Lucia Galovicova, Jana Ziarovska, Miroslava Kacaniova
Summary: This study aimed to characterize the bacterial profiles and their association with selected semen quality traits among two chicken breeds. The bacterial load and antimicrobial resistance were evaluated, and several pro-inflammatory cytokines and antibacterial proteins were measured. The results showed differences in bacterial composition and antimicrobial resistance between the two chicken breeds, and the presence of certain bacteria was associated with increased inflammation and oxidative stress in the semen samples. These findings emphasize the importance of bacteriospermia in the poultry industry and the need for more comprehensive microbiological screening of semen samples for artificial insemination.
Article
Agriculture, Dairy & Animal Science
Supakorn Authaida, Ruthaiporn Ratchamak, Wuttigrai Boonkum, Vibuntita Chankitisakul
Summary: Selenium supplementation in the diet can decrease lipid peroxidation and improve sperm quality in aged roosters. Fresh semen quality is not affected by rooster age, but cryopreserved semen quality is influenced by both age and selenium supplementation.
Article
Agriculture, Dairy & Animal Science
Ewa Lukaszewicz, Anna Jerysz, Artur Kowalczyk
Summary: The effect of adding freeze-dried quail egg white and yolk to basic EK extender on the morphology and motility of chicken broiler breeder semen was investigated.Study found that the addition of 200 mg/ml of egg white and 100 mg/ml of egg yolk to the EK extender resulted in the highest number of live normal sperm, while the neat semen had the highest sperm motility.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Biochemistry & Molecular Biology
Stefan Banas, Filip Benko, Michal Duracka, Norbert Lukac, Eva Tvrda
Summary: EPC has been shown to improve the quality parameters of cryopreserved bovine sperm, reduce the proportion of capacitated sperm, and decrease the production of reactive oxygen species. Additionally, EPC protects the integrity of the sperm membrane and membrane channels crucial for the sperm capacitation process. These findings suggest that EPC can enhance the structural integrity and functional activity of spermatozoa after cryopreservation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Marko Halo Jr, Filip Tirpak, Tomas Slanina, Katarina Tokarova, Martin Massanyi, Lucia Dianova, Eva Mlynekova, Agnieszka Gren, Marko Halo, Peter Massanyi
Summary: This study aimed to determine the impact of different taurine and caffeine combinations on the motility, viability, and oxidative markers of chilled stallion spermatozoa. The results showed that samples treated with the combination of taurine and caffeine significantly improved the motility parameters, without compromising viability or altering redox status. These findings suggest that the combination of taurine and caffeine can be an optimal supplement for improving the quality of stallion semen during chilled storage.
Article
Food Science & Technology
Lucia Zuscikova, Hana Greifova, Nikola Knizatova, Eva Tvrda, Anton Kovacik, Norbert Lukac, Tomas Jambor
Summary: This study investigated the potential effects of Tribulus terrestris L. extract on mouse Leydig cell parameters, showing significant impact on cell viability, membrane integrity, lysosomal activity, and hormone secretion at certain concentrations.
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2022)
Article
Food Science & Technology
Alzbeta Demianova, Alica Bobkova, Katarina Polakova, Lukas Jurcaga, Marek Bobko, Judita Lidikova, Andrea Mesarosova, Lubomir Belej, Ondrej Bucko
Summary: This research analyzed 21 samples of green Coffea arabica from different locations in Central America, focusing on parameters such as moisture content, water activity, textural features, and color. Results showed that moisture content did not exceed 14%, water activity ranged from 0.391 to 0.582, and there was a significant positive correlation between moisture content and water activity. Additionally, a negative correlation between water activity and fracturability was observed.
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2023)
Article
Physiology
Tomas Jambor, Nikola Knizatova, Hana Greifova, Anton Kovacik, Norbert Lukac
Summary: The aim of this study was to investigate the potential impacts of bisphenol A (BPA) and its analogues BPB, BPF, and BPS on mice TM3 Leydig cells, focusing on basal cell viability parameters such as metabolic activity, cell membrane integrity, and lysosomal activity after 48-h exposure. The study also evaluated the effects of bisphenols on ROS production, gap junctional intercellular communication (GJIC), and testosterone secretion. The results showed that activity started at 10 pg/ml of bisphenols after 48-h exposure, and significant ROS production was observed at 5 and 10 pg/ml. Moreover, a significant inhibition of GJIC was confirmed at 10, 25, and 50 pg/ml.
PHYSIOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Ruchi Vyas, Kavindra Kumar Kesari, Norbert Lukac, Petr Slama, Shubhadeep Roychoudhury, Rashmi Sisodia
Summary: This study evaluated the effect of ethanolic extract of Chlorophytum borivilianum L. (CRE) on sperm maturation against adversities posed by ionizing gamma irradiation. The results showed that CRE exhibited remarkable antioxidant potential and could protect sperm from oxidative stress.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Food Science & Technology
Natalia Ceryova, Judita Lidikova, Eduard Pinter, Marek Snirc, Hana Frankova, Monika Norbova, Silvia Fedorkova
Summary: Garlic, used as both food and traditional medicine, was analyzed for its nutritional properties and chemical composition in this study. Research on traditional crops like garlic is important as modern consumers seek foods with positive effects on human health and knowledge of these properties can support cultivation of cultivars with the highest content of bioactive compounds.
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2023)
Article
Cell Biology
Hana Duranova, Veronika Fialkova, Veronika Simora, Jana Bilcikova, Peter Massanyi, Norbert Lukac, Zuzana Knazicka
Summary: The impacts of iron enrichment and overload on the ultrastructural characteristics of human adrenocarcinoma NCIH295R cell line were evaluated. It was found that both untreated and iron-exposed cell populations exhibited similar ultrastructural features related to the steroidogenic process. However, low concentration of iron showed beneficial effects on the ultrastructural organization of the cells, while high concentration did not induce obvious modifications.
Article
Plant Sciences
Marek Snirc, Judita Lidikova, Natalia Ceryova, Eduard Pinter, Eva Ivanisova, Janette Musilova, Alena Vollmannova, Samuel Rybnikar
Summary: This study determined the content of macroelements, microelements, and phenolic acids in different cultivars of garlic. The results showed that Bjetin had a high content of minerals, while Garpel and Mojmir had higher levels of phenolic acids. Novozamocky was found to be rich in chlorogenic acid.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Food Science & Technology
Katarina Polakova, Alica Bobkova, Alzbeta Demianova, Marek Bobko, Judita Lidikova, Lukas Jurcaga, Lubomir Belej, Andrea Mesarosova, Melina Korcok, Tomas Toth
Summary: Coffee processing contributes significantly to food and product waste. Utilizing coffee co-products can address environmental issues, nutritionally unbalanced foods, population growth, and food-related diseases. This research examined the quality and sensory parameters of cascara (coffee husks) and silverskin from different botanical origins, revealing their potential as valuable sources of TAC, fiber, and other beneficial compounds. The sensory evaluation, however, indicated that none of the samples met the acceptable index value of 70%.
Review
Environmental Sciences
Lucia Zuscikova, Denis Bazany, Hana Greifova, Nikola Knizatova, Anton Kovacik, Norbert Lukac, Tomas Jambor
Summary: Neonicotinoids are widely used insecticides in crop protection due to their high toxicity to pests and ease of application, but their persistent nature and systemic spread raise concerns about environmental contamination and non-target organism toxicity. Acetamiprid, a new generation insecticide with low toxicity to honeybees, is considered a safer alternative. However, extensive usage of acetamiprid has also negatively impacted non-target animals. This review summarizes animal studies that investigated the toxicity of specific neonicotinoids, highlighting reproductive system disruptions and metabolic changes in both male and female organisms.
Article
Biology
Hana Greifova, Katarina Tokarova, Tomas Jambor, Nikola Stefunkova, Ivana Spevakova, Rudolf Dupak, Olha Balytska, Denis Bazany, Marcela Capcarova, Norbert Lukac
Summary: Isorhamnetin has anti-inflammatory, anti-proliferative, and chemoprotective properties. It suppresses the proliferation of human colon adenocarcinoma cells by increasing the generation of superoxide radicals and decreasing the production of interleukin 8 (IL-8) to exert an anti-inflammatory effect.
Article
Food Science & Technology
Erika Zetochova, Ivana Tirdiova, Alena Vollmannova, Janette Musilova, Judita Lidikova, Tatiana Bojnanska
Summary: The influence of biologically effective preparations on the content of phenolic compounds and the total polyphenolics content in legumes was evaluated. The results showed that the combination treatment of Rizobin LF and Lexin had the highest content of phenolic compounds in white lupine. The lowest content of caffeic acid, trans-ferulic acid, and myricetin in white lupine was found in the variant B with Rizobin LF. The highest total polyphenolics content in grass pea was observed in the control variant A.
JOURNAL OF FOOD AND NUTRITION RESEARCH
(2023)
Article
Food Science & Technology
Janette Musilova, Silvia Fedorkova, Klara Podhorecka, Lubos Harangozo, Andrea Mesarosova, Alena Vollmannova, Judita Lidikova, Natalia Ceryova, Monika Norbova, Matyas Orsak
Summary: The cultivation of sweet chestnuts in Slovakia has a long tradition, but there is limited research on the chemical composition of different varieties in individual growing areas. The content of carbohydrates and mineral substances in sweet chestnuts is mainly influenced by variety and age.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Meeting Abstract
Agriculture, Dairy & Animal Science
M. Duracka, L. Galovicova, S. Kunova, M. Kacaniova, N. Lukac, E. Tvrda
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
