4.4 Article

Evaluation of DNA damage in the root cells of Allium cepa seeds growing in soil of high background radiation areas of Ramsar -: Iran

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JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
卷 99, 期 10, 页码 1698-1702

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ELSEVIER SCI LTD
DOI: 10.1016/j.jenvrad.2008.03.013

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comet assay; DNA damage; Allium cepa; high background radiation areas (HBRAs); Ramsar

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Plants are unique in their ability to serve as in situ monitors for environmental genotoxins. We have used the alkaline comet assay for detecting induced DNA damage in Allium cepa to estimate the impact of high levels of natural radiation in the soils of inhabited zones of Ramsar. The average specific activity of natural radionuclides measured in the soil samples for Ra-226 was 12,766 Bq kg(-1) whereas in the control soils was in the range of 34-60 Bq kg(-1). A positive strong significant correlation of the DNA damage in nuclei of the root cells of A. cepa seeds germinated in the soil of high background radiation areas with Ra-226 specific activity of the soil samples was observed. The results showed high genotoxicity of radioactively contaminated soils. Also the linear increase in the DNA damage indicates that activation of repair enzymes is not triggered by exposure to radiation in HBRA. (c) 2008 Elsevier Ltd. All rights reserved.

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