Article
Chemistry, Analytical
Saumya Singh, Taru Verma, Balaram Khamari, Eswarappa Pradeep Bulagonda, Dipankar Nandi, Siva Umapathy
Summary: There has been a significant increase in antibiotic-resistant bacteria recently, and conventional diagnostic techniques are time-consuming and labor-intensive. Therefore, researchers investigated the potential of Raman spectroscopy as a rapid surveillance technology for tracking antibiotic resistance. The study used Raman spectroscopy to differentiate between antibiotic-resistant and sensitive bacteria, and the changes observed in the spectra correlated with antimicrobial susceptibility test results. This study demonstrates the potential of Raman spectroscopy for detecting antibiotic resistance.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Liping Li, Francesca L. Short, Karl A. Hassan, Varsha Naidu, Alaska Pokhrel, Stephanie S. Nagy, Farzana T. Prity, Bhumika S. Shah, Nusrat Afrin, Stephen Baker, Julian Parkhill, Amy K. Cain, Ian T. Paulsen
Summary: Concerns have been raised regarding the potential contribution of antiseptic or disinfectant biocides to the emergence and spread of multidrug-resistant bacteria. This study used transposon-directed insertion-site sequencing to investigate the effects of a panel of biocides on the fitness of the multidrug-resistant pathogen Acinetobacter baumannii. The results suggest that these biocides may have intracellular targets in addition to their known effects on the cell envelope, and that residual biocides could promote antibiotic resistance in pathogenic bacteria.
NATURE MICROBIOLOGY
(2023)
Article
Medical Laboratory Technology
Rosanna Falbo, Giovanni Battista Fogazzi, Maria Roberta Sala, Giuseppe Garigali, Adela Sulejmani, Paolo Brambilla, Valerio Leoni
Summary: Spheroplasts can be easily misclassified as other components in urine sediment, but can be correctly identified and classified by well-trained professionals using automated urine sediment analyzers. This can lead to prompt targeted treatment for urinary tract infections.
CLINICA CHIMICA ACTA
(2021)
Review
Microbiology
Herbert L. DuPont
Summary: Rifaximin-alpha is a gut-targeted antibiotic with multifaceted mechanism of action that not only exhibits direct antimicrobial effects but also improves gut permeability and reduces inflammation. It has low systemic drug levels and low risk of resistance compared to other antibiotics. Its efficacy and safety have been observed in chronic gastrointestinal disorders and traveler's diarrhea.
CLINICAL MICROBIOLOGY REVIEWS
(2023)
Article
Infectious Diseases
Mahdawi Joya, Ahmad Khalid Aalemi, Abdul Tawab Baryali
Summary: The study aimed to identify common bacterial uropathogens and their susceptibility to commonly used antibiotics. The findings showed that E. coli was the most common bacterial uropathogen, followed by Enterococcus species. Antibiotic resistance poses a significant challenge in the treatment of urinary tract infections.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biochemical Research Methods
V. Lucien Barnes, Douglas M. Heithoff, Scott P. Mahan, John K. House, Michael J. Mahan
Summary: This article presents a protocol for evaluating the minimum inhibitory concentration (MIC) values of clinically relevant antibiotics against bacterial isolates cultured in standard bacteriologic medium and in mammalian cell culture medium. The protocol can potentially optimize the use of existing antibiotics while enhancing efforts to discover new ones.
Article
Infectious Diseases
Bruna Rossini Lara, Bruno Braidotti de Camargo, Claudete Rodrigues Paula, Gabrielle Pires de Morais Monari, Hans Garcia Garces, Mariana Volpe Arnoni, Monica Silveira, Viviane Mazo Favero Gimenes, Diniz Pereira Leite Junior, Lucas Xavier Bonfietti, Lidiane Oliveira, Marcia de Souza Carvalho Melhem, Marcos Auler, Regina Teixeira Barbieri Ramos, Amanda Latercia Tranches Dias, Naiara Chaves Silva, Debora Moreira, Virginia Bodelao Richini-Pereira, Lais Anversa, Luciana da Silva Ruiz
Summary: This study investigated the biofilm formation ability of 59 isolates of the medically important yeast Trichosporon and their susceptibility to antifungal agents. All isolates were able to form biofilms and showed lower susceptibility to antifungal drugs. Trichosporonosis is a systemic fungal disease with high mortality rates, commonly associated with medical devices and immunosuppressed patients. The susceptibility of biofilm cells to antifungals was higher than that of planktonic cells, and different methods for testing susceptibility showed discrepancies in minimum inhibitory concentrations, especially for fluconazole, itraconazole, and caspofungin. These findings highlight the need for further studies on Trichosporon spp. to better understand their characteristics and response to treatment.
Article
Dermatology
Marly Alejandra Gavilanes-Martinez, Alejandra Coral-Garzon, Diego H. Caceres, Ana Maria Garcia
Summary: This study found that boric acid and triclosan have antifungal activity against multiple Candida species, with some mixtures showing enhanced antifungal effects without antagonism. Further research is needed to determine if these compounds can reduce the burden of Candida on skin.
Review
Biochemistry & Molecular Biology
Surajit Bhattacharjya, Sk Abdul Mohid, Anirban Bhunia
Summary: Antimicrobial peptides (AMPs) are effective therapeutic modalities against drug-resistant Gram-negative bacteria. This review article discusses the 3-D structures and mode of actions of several potent AMPs, as well as studies on human peptide LL37 and de novo-designed peptides. The challenges in clinical trials of AMPs are also summarized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Marek Samec, Alena Liskova, Lenka Koklesova, Kevin Zhai, Elizabeth Varghese, Samson Mathews Samuel, Miroslava Sudomova, Vincent Lucansky, Monika Kassayova, Martin Pec, Kamil Biringer, Aranka Brockmueller, Karol Kajo, Sherif T. S. Hassan, Mehdi Shakibaei, Olga Golubnitschaja, Dietrich Busselberg, Peter Kubatka
Summary: Metabolic reprogramming is crucial for cancer development, and melatonin plays a significant role in inhibiting tumorigenesis and metastasis by regulating key enzymes and glucose transporters, among other pathways. Melatonin also modulates critical metabolic processes and signaling cascades, highlighting its potential as an innovative approach for cancer prevention and therapy.
Article
Microbiology
Elyse J. Roach, Tsuyoshi Uehara, Denis M. Daigle, David A. Six, Cezar M. Khursigara
Summary: Gram-negative bacteria producing carbapenemases are resistant to beta-lactam antibiotics, posing a significant health risk. Taniborbactam is an investigational broad-spectrum beta-lactamase inhibitor effective against serine- and metallo-beta-lactamases, including KPC. This study demonstrated the effectiveness of taniborbactam in restoring the antibacterial activity of cefepime against KPC-3-producing Escherichia coli, highlighting its potential in combating beta-lactamase-mediated antibiotic resistance.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Michaela Tutone, Truls E. Bjerklund Johansen, Tommaso Cai, Shazad Mushtaq, David M. Livermore
Summary: Urinary tract infections (UTIs) are common worldwide, especially among women. A study investigated the susceptibility of current urinary isolates to fosfomycin and other antibiotics, and found that Escherichia coli was the most common uropathogen isolated and highly susceptible to fosfomycin, nitrofurantoin, and mecillinam.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Shu-Chen Kuo, Mei-Chen Tan, Wei-Cheng Huang, Han-Chieh Wu, Feng-Jui Chen, Yu-Chieh Liao, Hui-Ying Wang, Yih-Ru Shiau, Tsai-Ling Lauderdale
Summary: The study aimed to determine the susceptibilities of Elizabethkingia spp. to various antibiotics, revealing differences in susceptibility among different strains. Discrepancies were observed between the results obtained from traditional methods and an automated system.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Sonia Prieto Martin Gil, Ana Tajuelo, Mireia Lopez-Siles, Michael J. McConnell
Summary: This study revealed that subinhibitory concentrations of clinically-relevant antibiotics and disinfectants have variable effects on the promoter activity of RND family members in Acinetobacter baumannii, depending on the strain, compound tested, and growth phase. These findings suggest important implications for the treatment of A. baumannii infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Yingjie Qiao, Jiayi He, Ping Han, Jiangbo Qu, Xubo Wang, Jun Wang
Summary: In this study, adult zebrafish were exposed to triclosan for 150 days, and it was found that long-term exposure to triclosan caused endocrine disruption, reduced germ cell count, and decreased hatching rate and survival rate of offspring. This research provided important insights into the potential diseases caused by triclosan exposure in the aquatic environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)